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リコンビナントRabMAb

Anti-ALK (phospho Y1604) 抗体 [EPR24750-116] - BSA and Azide free (ab290748)

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Western blot - Anti-ALK (phospho Y1604) antibody [EPR24750-116] - BSA and Azide free (ab290748)
  • Dot Blot - Anti-ALK (phospho Y1604) antibody [EPR24750-116] - BSA and Azide free (ab290748)
  • Immunoprecipitation - Anti-ALK (phospho Y1604) antibody [EPR24750-116] - BSA and Azide free (ab290748)
  • Anti-ALK (phospho Y1604) antibody [EPR24750-116] - BSA and Azide free (ab290748)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR24750-116] to ALK (phospho Y1604) - BSA and Azide free
  • Suitable for: WB, Dot blot, IP
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Unconjugated

製品の概要

  • 製品名

    Anti-ALK (phospho Y1604) antibody [EPR24750-116] - BSA and Azide free
    ALK 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR24750-116] to ALK (phospho Y1604) - BSA and Azide free

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, Dot blot, IPmore details
    適用なし: Flow Cyt (Intra),ICC/IF or IHC-P

  • 種交差性

    交差種: Human

  • 免疫原

    Synthetic peptide corresponding to Human ALK aa 1600-1700 (phospho Y1604).
    Database link: Q9UM73

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • ポジティブ･コントロール

    • WB: KARPAS-299 (human anaplastic large cell lymphoma) whole cell fresh lysate (Untreated membrane), KARPAS-299 treated with 1 mM pervanadate for 30 minutes, whole cell fresh lysate (Untreated membrane). IP: KARPAS-299 (human anaplastic large cell lymphoma) treated with 50 µM pervanadate for 30 minutes, whole cell fresh lysate.

  • 特記事項

    ab290748 is a carrier-free version of ab290733. 

     

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab290748の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
Use at an assay dependent concentration. Predicted molecular weight: 176 kDa.
Dot blot
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
特記事項
WB
Use at an assay dependent concentration. Predicted molecular weight: 176 kDa.
Dot blot
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
追加情報
Is unsuitable for Flow Cyt (Intra),ICC/IF or IHC-P.

ターゲット情報

  • 機能

    Neuronal receptor tyrosine kinase that is essentially and transiently expressed in specific regions of the central and peripheral nervous systems and plays an important role in the genesis and differentiation of the nervous system. Transduces signals from ligands at the cell surface, through specific activation of the mitogen-activated protein kinase (MAPK) pathway. Phosphorylates almost exclusively at the first tyrosine of the Y-x-x-x-Y-Y motif. Following activation by ligand, ALK induces tyrosine phosphorylation of CBL, FRS2, IRS1 and SHC1, as well as of the MAP kinases MAPK1/ERK2 and MAPK3/ERK1. Acts as a receptor for ligands pleiotrophin (PTN), a secreted growth factor, and midkine (MDK), a PTN-related factor, thus participating in PTN and MDK signal transduction. PTN-binding induces MAPK pathway activation, which is important for the anti-apoptotic signaling of PTN and regulation of cell proliferation. MDK-binding induces phosphorylation of the ALK target insulin receptor substrate (IRS1), activates mitogen-activated protein kinases (MAPKs) and PI3-kinase, resulting also in cell proliferation induction. Drives NF-kappa-B activation, probably through IRS1 and the activation of the AKT serine/threonine kinase. Recruitment of IRS1 to activated ALK and the activation of NF-kappa-B are essential for the autocrine growth and survival signaling of MDK.

  • 組織特異性

    Expressed in brain and CNS. Also expressed in the small intestine and testis, but not in normal lymphoid cells.

  • 関連疾患

    A chromosomal aberration involving ALK is found in a form of non-Hodgkin lymphoma. Translocation t(2;5)(p23;q35) with NPM1. The resulting chimeric NPM1-ALK protein homodimerize and the kinase becomes constitutively activated. The constitutively active fusion proteins are responsible for 5-10% of non-Hodgkin lymphomas.
    A chromosomal aberration involving ALK is associated with inflammatory myofibroblastic tumors (IMTs). Translocation t(2;11)(p23;p15) with CARS; translocation t(2;4)(p23;q21) with SEC31A.
    A chromosomal aberration involving ALK is associated with anaplastic large-cell lymphoma (ALCL). Translocation t(2;17)(p23;q25) with ALO17.
    Neuroblastoma 3
    The ALK signaling pathway plays an important role in glioblastoma, the most common malignant brain tumor of adults and one of the most lethal cancers. It regulates both glioblastoma migration and growth.
    A chromosomal aberration involving ALK is found in one subject with colorectal cancer. Translocation t(2;2)(p23.1;p23.3). A 5 million base pair tandem duplication generates an in-frame WDCP-ALK gene fusion.

  • 配列類似性

    Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.
    Contains 1 LDL-receptor class A domain.
    Contains 2 MAM domains.
    Contains 1 protein kinase domain.

  • 翻訳後修飾

    Phosphorylated at tyrosine residues by autocatalysis, which activates kinase activity. In cells not stimulated by a ligand, receptor protein tyrosine phosphatase beta and zeta complex (PTPRB/PTPRZ1) dephosphorylates ALK at the sites in ALK that are undergoing autophosphorylation through autoactivation. Phosphorylation at Tyr-1507 is critical for SHC1 association.
    N-glycosylated.

  • 細胞内局在

    Cell membrane. Membrane attachment was crucial for promotion of neuron-like differentiation and cell proliferation arrest through specific activation of the MAP kinase pathway.
  • Information by UniProt

  • 参照データベース

    • 別名

      • Alk antibody
      • ALK tyrosine kinase receptor antibody
      • ALK/EML4 fusion gene, included antibody
      • ALK/NPM1 fusion gene, included antibody
      • ALK_HUMAN antibody
      • anaplastic lymphoma kinase (Ki-1) antibody
      • Anaplastic lymphoma kinase antibody
      • Anaplastic lymphoma kinase Ki1 antibody
      • anaplastic lymphoma receptor tyrosine kinase antibody
      • CD 246 antibody
      • CD246 antibody
      • CD246 antigen antibody
      • EC 2.7.10.1 antibody
      • Ki 1 antibody
      • Ki1 antibody
      • mutant anaplastic lymphoma kinase antibody
      • NBLST 3 antibody
      • NBLST3 antibody
      • Tcrz antibody
      • TFG/ALK antibody
      see all

    画像

    • Western blot - Anti-ALK (phospho Y1604) antibody [EPR24750-116] - BSA and Azide free (ab290748)
      Western blot - Anti-ALK (phospho Y1604) antibody [EPR24750-116] - BSA and Azide free (ab290748)
      All lanes : Anti-ALK (phospho Y1604) antibody [EPR24750-116] (ab290733) at 1/1000 dilution

      Lane 1 : KARPAS-299 (human anaplastic large cell lymphoma) whole cell fresh lysate (Untreated membrane)
      Lane 2 : KARPAS-299 treated with 1 mM pervanadate for 30 minutes, whole cell fresh lysate (Untreated membrane)
      Lane 3 : KARPAS-299 whole cell fresh lysate (phosphatase treated membrane)
      Lane 4 : KARPAS-299 treated with 1 mM pervanadate for 30 minutes, whole cell fresh lysate (phosphatase treated membrane)

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 176 kDa
      Observed band size: 80 kDa why is the actual band size different from the predicted?


      Exposure time: 26 seconds


      This data was developed using ab290733, the same antibody clone in a different buffer formulation. 

      Blocking / Diluent buffer and concentration: 5% NFDM/TBST

      KARPAS-299 cells carry the NPM-ALK fusion (PMID: 32193476, 9736036).  Phosphorylated Tyr664 of NPM-ALK is equivalent to Tyr1604 of full length ALK.

      Lysates were prepared from fresh material and used for Western blotting immediately to minimize protein degradation.

    • Dot Blot - Anti-ALK (phospho Y1604) antibody [EPR24750-116] - BSA and Azide free (ab290748)
      Dot Blot - Anti-ALK (phospho Y1604) antibody [EPR24750-116] - BSA and Azide free (ab290748)

      This data was developed using ab290733, the same antibody clone in a different buffer formulation.

      Dot blot analysis of ALK (Phospho Y1604) using ab290733 at 1:1000 (0.549 μg/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1:100,000 dilution.

      Exposure time: 3 minutes

      Blocking and diluting buffer and concentration: 5% NFDM/TBST

    • Immunoprecipitation - Anti-ALK (phospho Y1604) antibody [EPR24750-116] - BSA and Azide free (ab290748)
      Immunoprecipitation - Anti-ALK (phospho Y1604) antibody [EPR24750-116] - BSA and Azide free (ab290748)

      This data was developed using ab290733, the same antibody clone in a different buffer formulation.

      ALK (Phospho Y1604) was immunoprecipitated from 0.35 mg KARPAS-299 (human anaplastic large cell lymphoma) treated with 50 μM pervanadate for 30 minutes, whole cell fresh lysate 10 μg with ab290733 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab290733 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

      Lane 1: KARPAS-299 (human anaplastic large cell lymphoma) treated with 50 μM pervanadate for 30 minutes, whole cell fresh lysate 10 μg

      Lane 2: ab290733 IP in KARPAS-299 treated with 50 μM pervanadate for 30 minutes, whole cell fresh lysate

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab290733 in KARPAS-299 treated with 50 μM pervanadate for 30 minutes, whole cell fresh lysate

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 3.25 seconds

      The lower bands in lane 2 may be caused by degradation. Lysates were prepared from fresh material and used for IP immediately to minimize protein degradation.

    • Anti-ALK (phospho Y1604) antibody [EPR24750-116] - BSA and Azide free (ab290748)
      Anti-ALK (phospho Y1604) antibody [EPR24750-116] - BSA and Azide free (ab290748)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab290748 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab290748 は論文での使用が確認できていません。

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