Anti-ALK (phospho Y1604) 抗体 [EPR24750-116] (ab290733)
![Western blot - Anti-ALK (phospho Y1604) antibody [EPR24750-116] (ab290733)](https://www.abcam.co.jp/ps/products/290/ab290733/Images/ab290733-484798-anti-ALK-phospho-Y1604-antibody-EPR24750116-western-blot-human.jpg "Western blot - Anti-ALK (phospho Y1604) antibody [EPR24750-116] (ab290733)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR24750-116] to ALK (phospho Y1604)
- Suitable for: WB, Dot blot, IP
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-ALK (phospho Y1604) antibody [EPR24750-116]
ALK 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR24750-116] to ALK (phospho Y1604) -
由来種
Rabbit -
アプリケーション
適用あり: WB, Dot blot, IPmore details
適用なし: Flow Cyt (Intra),ICC/IF or IHC-P -
種交差性
交差種: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- WB: KARPAS-299 (human anaplastic large cell lymphoma) whole cell fresh lysate (Untreated membrane), KARPAS-299 treated with 1 mM pervanadate for 30 minutes, whole cell fresh lysate (Untreated membrane). IP: KARPAS-299 (human anaplastic large cell lymphoma) treated with 50 µM pervanadate for 30 minutes, whole cell fresh lysate.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR24750-116 -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab290733の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000. Predicted molecular weight: 176 kDa.
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| Dot blot |
1/1000.
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| IP |
1/30.
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| 特記事項 |
|---|
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WB
1/1000. Predicted molecular weight: 176 kDa. |
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Dot blot
1/1000. |
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IP
1/30. |
ターゲット情報
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機能
Neuronal receptor tyrosine kinase that is essentially and transiently expressed in specific regions of the central and peripheral nervous systems and plays an important role in the genesis and differentiation of the nervous system. Transduces signals from ligands at the cell surface, through specific activation of the mitogen-activated protein kinase (MAPK) pathway. Phosphorylates almost exclusively at the first tyrosine of the Y-x-x-x-Y-Y motif. Following activation by ligand, ALK induces tyrosine phosphorylation of CBL, FRS2, IRS1 and SHC1, as well as of the MAP kinases MAPK1/ERK2 and MAPK3/ERK1. Acts as a receptor for ligands pleiotrophin (PTN), a secreted growth factor, and midkine (MDK), a PTN-related factor, thus participating in PTN and MDK signal transduction. PTN-binding induces MAPK pathway activation, which is important for the anti-apoptotic signaling of PTN and regulation of cell proliferation. MDK-binding induces phosphorylation of the ALK target insulin receptor substrate (IRS1), activates mitogen-activated protein kinases (MAPKs) and PI3-kinase, resulting also in cell proliferation induction. Drives NF-kappa-B activation, probably through IRS1 and the activation of the AKT serine/threonine kinase. Recruitment of IRS1 to activated ALK and the activation of NF-kappa-B are essential for the autocrine growth and survival signaling of MDK. -
組織特異性
Expressed in brain and CNS. Also expressed in the small intestine and testis, but not in normal lymphoid cells. -
関連疾患
A chromosomal aberration involving ALK is found in a form of non-Hodgkin lymphoma. Translocation t(2;5)(p23;q35) with NPM1. The resulting chimeric NPM1-ALK protein homodimerize and the kinase becomes constitutively activated. The constitutively active fusion proteins are responsible for 5-10% of non-Hodgkin lymphomas.
A chromosomal aberration involving ALK is associated with inflammatory myofibroblastic tumors (IMTs). Translocation t(2;11)(p23;p15) with CARS; translocation t(2;4)(p23;q21) with SEC31A.
A chromosomal aberration involving ALK is associated with anaplastic large-cell lymphoma (ALCL). Translocation t(2;17)(p23;q25) with ALO17.
Neuroblastoma 3
The ALK signaling pathway plays an important role in glioblastoma, the most common malignant brain tumor of adults and one of the most lethal cancers. It regulates both glioblastoma migration and growth.
A chromosomal aberration involving ALK is found in one subject with colorectal cancer. Translocation t(2;2)(p23.1;p23.3). A 5 million base pair tandem duplication generates an in-frame WDCP-ALK gene fusion. -
配列類似性
Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.
Contains 1 LDL-receptor class A domain.
Contains 2 MAM domains.
Contains 1 protein kinase domain. -
翻訳後修飾
Phosphorylated at tyrosine residues by autocatalysis, which activates kinase activity. In cells not stimulated by a ligand, receptor protein tyrosine phosphatase beta and zeta complex (PTPRB/PTPRZ1) dephosphorylates ALK at the sites in ALK that are undergoing autophosphorylation through autoactivation. Phosphorylation at Tyr-1507 is critical for SHC1 association.
N-glycosylated. -
細胞内局在
Cell membrane. Membrane attachment was crucial for promotion of neuron-like differentiation and cell proliferation arrest through specific activation of the MAP kinase pathway. - Information by UniProt
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参照データベース
- Entrez Gene: 238 Human
- Omim: 105590 Human
- SwissProt: Q9UM73 Human
- Unigene: 654469 Human
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別名
- Alk antibody
- ALK tyrosine kinase receptor antibody
- ALK/EML4 fusion gene, included antibody
see all
画像
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Western blot - Anti-ALK (phospho Y1604) antibody [EPR24750-116] (ab290733)All lanes : Anti-ALK (phospho Y1604) antibody [EPR24750-116] (ab290733) at 1/1000 dilution
Lane 1 : KARPAS-299 (human anaplastic large cell lymphoma) whole cell fresh lysate (Untreated membrane)
Lane 2 : KARPAS-299 treated with 1 mM pervanadate for 30 minutes, whole cell fresh lysate (Untreated membrane)
Lane 3 : KARPAS-299 whole cell fresh lysate (phosphatase treated membrane)
Lane 4 : KARPAS-299 treated with 1 mM pervanadate for 30 minutes, whole cell fresh lysate (phosphatase treated membrane)
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 176 kDa
Observed band size: 80 kDa why is the actual band size different from the predicted?
Exposure time: 26 secondsBlocking / Diluent buffer and concentration: 5% NFDM/TBST
KARPAS-299 cells carry the NPM-ALK fusion (PMID: 32193476, 9736036). Phosphorylated Tyr664 of NPM-ALK is equivalent to Tyr1604 of full length ALK.
Lysates were prepared from fresh material and used for Western blotting immediately to minimize protein degradation. -
![Dot Blot - Anti-ALK (phospho Y1604) antibody [EPR24750-116] (ab290733)](https://www.abcam.co.jp/ps/products/290/ab290733/Images/ab290733-3-anti-alk-phospho-y1604-antibody-epr24750116-dotblot-alk-control-peptide.jpg)
Dot Blot - Anti-ALK (phospho Y1604) antibody [EPR24750-116] (ab290733)Dot blot analysis of ALK (Phospho Y1604) using ab290733 at 1:1000 (0.549 μg/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1:100,000 dilution.
Exposure time: 3 minutes
Blocking and diluting buffer and concentration: 5% NFDM/TBST
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![Immunoprecipitation - Anti-ALK (phospho Y1604) antibody [EPR24750-116] (ab290733)](https://www.abcam.co.jp/ps/products/290/ab290733/Images/ab290733-2-anti-alk-phospho-y1604-antibody-epr24750116-immunoprecipitation-karpas299.jpg)
Immunoprecipitation - Anti-ALK (phospho Y1604) antibody [EPR24750-116] (ab290733)ALK (Phospho Y1604) was immunoprecipitated from 0.35 mg KARPAS-299 (human anaplastic large cell lymphoma) treated with 50 μM pervanadate for 30 minutes, whole cell fresh lysate 10 μg with ab290733 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab290733 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: KARPAS-299 (human anaplastic large cell lymphoma) treated with 50 μM pervanadate for 30 minutes, whole cell fresh lysate 10 μg
Lane 2: ab290733 IP in KARPAS-299 treated with 50 μM pervanadate for 30 minutes, whole cell fresh lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab290733 in KARPAS-299 treated with 50 μM pervanadate for 30 minutes, whole cell fresh lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3.25 seconds
The lower bands in lane 2 may be caused by degradation. Lysates were prepared from fresh material and used for IP immediately to minimize protein degradation.
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![Anti-ALK (phospho Y1604) antibody [EPR24750-116] (ab290733)](https://www.abcam.co.jp/ps/products/290/ab290733/Images/ab290733-484504-Benefits-of-Recombinant-antibodies.jpg)
Anti-ALK (phospho Y1604) antibody [EPR24750-116] (ab290733)
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (0)
ab290733 は論文での使用が確認できていません。