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Anti-ALK-1 抗体 [MM0015-8G33] (ab51870)

Reviews (1)Q&A (2)References (4)
Western blot - Anti-ALK-1 antibody [MM0015-8G33] (ab51870)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALK-1 antibody [MM0015-8G33] (ab51870)
  • Flow Cytometry - Anti-ALK-1 antibody [MM0015-8G33] (ab51870)

Key features and details

  • Mouse monoclonal [MM0015-8G33] to ALK-1
  • Suitable for: IHC-P, WB, Flow Cyt
  • Reacts with: Human
  • Isotype: IgG2a

製品の概要

  • 製品名

    Anti-ALK-1 antibody [MM0015-8G33]
    ALK-1 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [MM0015-8G33] to ALK-1

  • 由来種

    Mouse

  • アプリケーション

    適用あり: IHC-P, WB, Flow Cytmore details

  • 種交差性

    交差種: Human

  • 免疫原

    Recombinant full length protein corresponding to Human ALK-1 (extracellular).
    Database link: P37023

  • ポジティブ･コントロール

    • WB: Human placenta tissue lysate. IHC-P: Human placenta tissue. Flow Cyt: JEG3 cells.

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab51870の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-P
1/50.
WB
1/200 - 1/800. Predicted molecular weight: 56 kDa.
Flow Cyt
Use 1µg for 106 cells.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. 
特記事項
IHC-P
1/50.
WB
1/200 - 1/800. Predicted molecular weight: 56 kDa.
Flow Cyt
Use 1µg for 106 cells.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. 

ターゲット情報

  • 機能

    On ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. Receptor for TGF-beta. May bind activin as well.

  • 関連疾患

    Defects in ACVRL1 are the cause of hereditary hemorrhagic telangiectasia type 2 (HHT2) [MIM:600376]; also known as Osler-Rendu-Weber syndrome 2 (ORW2). HHT2 is an autosomal dominant multisystemic vascular dysplasia, characterized by recurrent epistaxis, muco-cutaneous telangiectases, gastro-intestinal hemorrhage, and pulmonary, cerebral and hepatic arteriovenous malformations; all secondary manifestations of the underlying vascular dysplasia.

  • 配列類似性

    Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. TGFB receptor subfamily.
    Contains 1 GS domain.
    Contains 1 protein kinase domain.

  • 細胞内局在

    Membrane.
  • Information by UniProt

  • 参照データベース

    • 別名

      • Activin A receptor antibody
      • Activin A receptor type II like 1 antibody
      • Activin A receptor, type II like kinase 1 antibody
      • Activin receptor like kinase 1 antibody
      • Activin receptor-like kinase 1 antibody
      • ACVL1_HUMAN antibody
      • ACVRL1 antibody
      • ACVRLK1 antibody
      • ALK-1 antibody
      • ALK1 antibody
      • HHT antibody
      • HHT2 antibody
      • ORW2 antibody
      • Osler Rendu Weber syndrome 2 antibody
      • Serine/threonine protein kinase receptor R3 antibody
      • Serine/threonine-protein kinase receptor R3 antibody
      • SKR3 antibody
      • TGF B superfamily receptor type I antibody
      • TGF-B superfamily receptor type I antibody
      • TSR-I antibody
      • TSR1 antibody
      see all

    画像

    • Western blot - Anti-ALK-1 antibody [MM0015-8G33] (ab51870)
      Western blot - Anti-ALK-1 antibody [MM0015-8G33] (ab51870)
      Anti-ALK-1 antibody [MM0015-8G33] (ab51870) at 1/200 dilution + Human placenta tissue lysate

      Predicted band size: 56 kDa
      Observed band size: 50 kDa why is the actual band size different from the predicted?

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALK-1 antibody [MM0015-8G33] (ab51870)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALK-1 antibody [MM0015-8G33] (ab51870)

      ab51870 at 1/50 dilution staining ALK-1 in Human placental tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections).

    • Flow Cytometry - Anti-ALK-1 antibody [MM0015-8G33] (ab51870)
      Flow Cytometry - Anti-ALK-1 antibody [MM0015-8G33] (ab51870)

      Overlay histogram showing JEG3 cells stained with ab51870 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab51870, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in JEG3 cells fixed with 4% paraformaldehyde (10 min) used under the same conditions.

      Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

    参考文献 (4)

    ab51870 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab51870 は 4 報の論文で使用されています。

    • Gupta A  et al. Cilia proteins are biomarkers of altered flow in the vasculature. JCI Insight 7:N/A (2022). PubMed: 35143420
    • Nakamura I  et al. Sulfatase-2 Regulates Liver Fibrosis through the TGF-β Signaling Pathway. Cancers (Basel) 13:N/A (2021). PubMed: 34771445
    • Tengroth L  et al. Activation of Activin receptor-like kinases curbs mucosal inflammation and proliferation in chronic rhinosinusitis with nasal polyps. Sci Rep 8:1561 (2018). PubMed: 29367682
    • Wang HX  et al. EWI-2 negatively regulates TGF-ß signaling leading to altered melanoma growth and metastasis. Cell Res 25:370-85 (2015). PubMed: 25656846

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-3 of 3 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-ALK-1 antibody [MM0015-8G33]

     Inconclusive
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Placenta)
    Antigen retrieval step
    Heat mediated
    Permeabilization
    No
    Specification
    Placenta
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    MR. Carl Hobbs

    Verified customer

    投稿 Jun 27 2013

    Question

    The TGFBRI construct I am using contains the full length protein, 503 amino acids long, with one GFP tag at the c-terminal end of the protein.

    I had my construct sequence verified and aside from one point mutation, the sequence is identical to the wild type. I checked the data sheet for the antibody and it has been engineered to recognise the amino acid sequence : VPNEEDPSLDRPFISEGTTLKD. As you can see from the sequencing data this sequence is correct in my construct.

    I have attached a document containing sequencing data from my construct. TGFBRI WT represents the cDNA of the wildtype protein, and TGFBRI_wt_R is the sequence of my construct using a reverse sequencing primer, data obtained from Macrogen.

    I was wondering if you have a list of publications where the authors have used this antibody for immunofluorescence in human cells? I was also wondering how long this antibody has been on the market for?

    Many thanks,

    Read More
    Answer

    Thank you very much for getting back to me and for confirming the construct details.

    If you have a chance, it would be still worth running a good positive control (cytospin preparation from A549 cells or tissue section from placenta) which endogenously express TGF beta Receptor I. This product was launched in March 2006 so it has been on the market for several years.

    There are 8 specific publications and the following papers have been used in immunostaining:

    Devaux Y et al. Transforming growth factor ß receptor 1 is a new candidate prognostic biomarker after acute myocardial infarction. BMC Med Genomics 4:83 (2011). IHC-Fr ; Rat .





    Sounni NE et al. Stromal regulation of vessel stability by MMP14 and TGFbeta. Dis Model Mech 3:317-32 (2010). IHC-Fr ; Mouse.





    Beckham JD et al. Reovirus activates transforming growth factor beta and bone morphogenetic protein signaling pathways in the central nervous system that contribute to neuronal survival following infection. J Virol 83:5035-45 (2009). IHC-P ; Mouse .



    I could offer you a new vial of ab31013 free of charge but we are waiting for a new stock. We have two alternative products against the same target TGF beta Receptor I. If you wish test either of them, I can send you a new vial free of charge.

    https://www.abcam.com/ab155258: Anti-TGF beta Receptor I antibody

    https://www.abcam.com/ab51870: Anti-TGF beta Receptor I antibody [MM0015-8G33]

    Let me know how you wish to proceed with your enquiry. I look forward to hearing from you soon.

    Read More

    Question

    Dear All,

    Please send me an offer for ab49575 Anti-TGF beta Receptor I antibody, Clone 8A1 - 200 ul q’ty 1 and for q’ty 10 CIP Skopje.

    Which clone of Anti-TGF beta Receptor I antibody do you recommend (Clone 8A1 or Clone MM0015-8G33) for IHC?

    Thank you in advance.

    Best regards

    Read More
    Answer

    Thank you for your enquiry which has been forwarded to our Scientific Support team.

    I would like to reassure you that our antibodies have been tested successfully and would be covered by our 6 month guarantee in the applications listed on the individual datasheets. Therefore, any TGF beta Receptor Iantibody tested in the species and application you are using should be suitable for your experiments.

    I can confirm that ab49575 clone [8A11] and ab51870 clone MM0015-8G33 have both been tested in IHC on paraffin embedded sections (IHC-P).

    Although we produce many in-house antibodies, we obtain other antibodies from a wide range of sources. Therefore, comparison experiments will not often have been done.

    If you wish to be more certain, I can suggest choosing the antibody that has been best characterized. By this I mean the antibody that has been tested in the most applications and has the most data available (on the datasheet).

    I hope this will be helpful. Should you have any further questions please do not hesitate to ask.

    Read More

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