Alexa Fluor® 790 Anti-beta Actin 抗体 [mAbcam 8226] - Loading Control (ab184576)
![Western blot - Alexa Fluor® 790 Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab184576)](https://www.abcam.co.jp/ps/products/184/ab184576/Images/ab184576-482445-alexa-fluor-790-anti-beta-actin-antibody-loading-control-western-blot.jpg "Western blot - Alexa Fluor® 790 Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab184576)")
Key features and details
- Alexa Fluor® 790 Mouse monoclonal [mAbcam 8226] to beta Actin - Loading Control
- Suitable for: WB
- Reacts with: Mouse, Human
- Conjugation: Alexa Fluor® 790. Ex: 782nm, Em: 805nm
- Isotype: IgG1
Related conjugates and formulations
製品の概要
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製品名
Alexa Fluor® 790 Anti-beta Actin antibody [mAbcam 8226] - Loading Control
beta Actin 一次抗体 製品一覧 -
製品の詳細
Alexa Fluor® 790 Mouse monoclonal [mAbcam 8226] to beta Actin - Loading Control -
由来種
Mouse -
標識
Alexa Fluor® 790. Ex: 782nm, Em: 805nm -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Mouse, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- WB: HeLa; HEK293; HepG2; NIH3T3 whole cell lysates
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特記事項
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. Store In the Dark. -
バッファー
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA -
Concentration information loading... -
ポリ/モノ
モノクローナル -
クローン名
mAbcam 8226 -
ミエローマ
Sp2/0-Ag14 -
アイソタイプ
IgG1 -
軽鎖の種類
kappa -
研究分野
関連製品
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Alternative Versions
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab184576の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (2) |
Use a concentration of 1 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).
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| 特記事項 |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa). |
ターゲット情報
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機能
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. -
関連疾患
Defects in ACTB are a cause of dystonia juvenile-onset (DYTJ) [MIM:607371]. DYTJ is a form of dystonia with juvenile onset. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYTJ patients manifest progressive, generalized, dopa-unresponsive dystonia, developmental malformations and sensory hearing loss. -
配列類似性
Belongs to the actin family. -
翻訳後修飾
ISGylated. -
細胞内局在
Cytoplasm > cytoskeleton. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. - Information by UniProt
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参照データベース
- Entrez Gene: 60 Human
- Entrez Gene: 11461 Mouse
- Omim: 102630 Human
- SwissProt: P60709 Human
- SwissProt: P60710 Mouse
- Unigene: 520640 Human
- Unigene: 708120 Human
- Unigene: 727576 Human
see all -
別名
- A26C1A antibody
- A26C1B antibody
- ACTB antibody
see all
画像
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Western blot - Alexa Fluor® 790 Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab184576)All lanes : Alexa Fluor® 790 Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab184576) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 4 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 42 kDa
Observed band size: 42 kDaThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Milk before being incubated with ab184576 overnight at 4°C. Antibody binding was detected after washing to remove excess antibody and imaged using the Licor Odyssey CLx.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab184576 は 2 報の論文で使用されています。
- Yan G et al. Decreased miR-124-3p promoted breast cancer proliferation and metastasis by targeting MGAT5. Am J Cancer Res 9:585-596 (2019). PubMed: 30949412
- Kang S et al. Abietic acid attenuates IL-1ß-induced inflammation in human osteoarthritis chondrocytes. Int Immunopharmacol 64:110-115 (2018). PubMed: 30172103