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Alexa Fluor® 790 Anti-alpha Tubulin 抗体 [DM1A] - Loading Control (ab184577)

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Western blot - Alexa Fluor® 790 Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab184577)

    Key features and details

    • Alexa Fluor® 790 Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control
    • Suitable for: WB
    • Reacts with: Mouse, Human
    • Conjugation: Alexa Fluor® 790. Ex: 782nm, Em: 805nm
    • Isotype: IgG1

    Conjugates logo Related conjugates and formulations

    Alexa Fluor® 488 Alexa Fluor® 594 Alexa Fluor® 680 Carrier Free FITC HRP Unconjugated

    製品の概要

    • 製品名

      Alexa Fluor® 790 Anti-alpha Tubulin antibody [DM1A] - Loading Control
      alpha Tubulin 一次抗体 製品一覧

    • 製品の詳細

      Alexa Fluor® 790 Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control

    • 由来種

      Mouse

    • 標識

      Alexa Fluor® 790. Ex: 782nm, Em: 805nm

    • アプリケーション

      適用あり: WBmore details

    • 種交差性

      交差種: Mouse, Human

    • エピトープ

      aa 426-450

    • ポジティブ･コントロール

      • This antibody gave a positive signal in the following whole cell lysates: HeLa; MCF7; U2OS; NIH3T3.

    • 特記事項

      Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    製品の特性

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab184577の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    WB (2)
    Use a concentration of 1 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).
    特記事項
    WB
    Use a concentration of 1 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).

    ターゲット情報

    • 機能

      Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.

    • 配列類似性

      Belongs to the tubulin family.

    • 翻訳後修飾

      Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
      Acetylation of alpha chains at Lys-40 stabilizes microtubules and affects affinity and processivity of microtubule motors. This modification has a role in multiple cellular functions, ranging from cell motility, cell cycle progression or cell differentiation to intracellular trafficking and signaling.

    • 細胞内局在

      Cytoplasm > cytoskeleton.
    • Information by UniProt

    • 参照データベース

      • 別名

        • Alpha-tubulin 1 antibody
        • ALS22 antibody
        • B ALPHA 1 antibody
        • bA408E5.3 antibody
        • H2 ALPHA antibody
        • Hum a tub1 antibody
        • Hum a tub2 antibody
        • LIS3 antibody
        • MGC171407 antibody
        • MGC55332 antibody
        • TBA4A_HUMAN antibody
        • Testis-specific alpha-tubulin antibody
        • TUBA1 antibody
        • TUBA1A antibody
        • tuba1l antibody
        • Tuba4a antibody
        • Tubulin alpha 1 chain antibody
        • Tubulin alpha antibody
        • Tubulin alpha-1 chain antibody
        • tubulin alpha-1B chain antibody
        • Tubulin alpha-4A chain antibody
        • Tubulin H2-alpha antibody
        • Tubulin, alpha 1 (testis specific) antibody
        • tubulin, alpha 1, like antibody
        • Tubulin, alpha 4a antibody
        • Tubulin, alpha, testis-specific antibody
        • Tubulin, alpha-1 antibody
        see all

      画像

      • Western blot - Alexa Fluor® 790 Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab184577)
        Western blot - Alexa Fluor® 790 Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab184577)
        All lanes : Alexa Fluor® 790 Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab184577) at 1 µg/ml

        Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
        Lane 2 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
        Lane 3 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate
        Lane 4 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate

        Lysates/proteins at 10 µg per lane.

        Predicted band size: 52 kDa
        Observed band size: 52 kDa



        This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Milk before being incubated with ab184577 overnight at 4°C. Antibody binding was detected after washing to remove excess antibody and imaged using the Licor Odyssey CLx.

      参考文献 (0)

      ab184577 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab184577 は論文での使用が確認できていません。

      レビューと Q&A

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      1-2 of 2 Abreviews or Q&A

      Western blot abreview for Alexa Fluor® 790 Anti-alpha Tubulin antibody [DM1A] - Loading Control

       Excellent
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Mouse Cell lysate - whole cell (mouse ES cells)
      Gel Running Conditions
      Reduced Denaturing
      Loading amount
      10 µg
      Specification
      mouse ES cells
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      投稿 Mar 18 2021

      Western blot abreview for Alexa Fluor® 790 Anti-alpha Tubulin antibody [DM1A] - Loading Control

       Excellent
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Human Cell lysate - whole cell (eHAP cells)
      Gel Running Conditions
      Reduced Denaturing (4-10% gradient gel)
      Loading amount
      5 µg
      Specification
      eHAP cells
      Blocking step
      Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      投稿 Mar 04 2021

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