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AB321310

Alexa Fluor® 750 Anti-TXNIP 抗体 [EPR14774]

Alexa Fluor® 750 Anti-TXNIP antibody [EPR14774]

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Rabbit Recombinant Monoclonal TXNIP antibody - conjugated to Alexa Fluor® 750.

別名を表示する

VDUP1, TXNIP, Thioredoxin-interacting protein, Thioredoxin-binding protein 2, Vitamin D3 up-regulated protein 1

関連する標識済み抗体及び組成の異なる製品 (6)

  • 578 PE

    PE Anti-TXNIP antibody [EPR14774]

  • 660 APC

    APC Anti-TXNIP antibody [EPR14774]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-TXNIP antibody [EPR14774]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-TXNIP antibody [EPR14774]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-TXNIP antibody [EPR14774]

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-TXNIP antibody [EPR14774]

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR14774

アイソタイプ

IgG

標識

Alexa Fluor® 750

励起波長/蛍光波長

Ex: 749nm, Em: 775nm

キャリアフリー

No

アプリケーション

Antibody Labelling, Target Binding Affinity

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

特異性

Expression levels of the target protein vary with sample type and some optimisation may be required. For western blotting, more concentrated lysates may be required when using tissues samples. Stimulation may be required to allow detection of the target protein due to low levels of endogenous expression in some samples. Please see images on parent for recommended treatment conditions and positive controls.

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製品の詳細

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

How are conjugated primary antibodies validated?
This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle|Store in the dark
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

TXNIP also known as thioredoxin-interacting protein or VDUP1 is an inhibitory protein that binds to and regulates the activity of thioredoxin. It has a molecular mass of around 46 kDa. TXNIP is expressed in various tissues including heart liver and skeletal muscle. It acts to control intracellular redox states by modulating thioredoxin’s antioxidant functions. TXNIP expression can vary according to oxidative stress and cellular metabolic conditions.
Biological function summary

TXNIP regulates cellular processes such as glucose metabolism stress response and apoptosis. It does not form a complex but functions by negatively regulating the activity of thioredoxin. This protein can lead to increased reactive oxygen species (ROS) when it inhibits thioredoxin influencing cellular redox balance. TXNIP therefore plays a regulatory role in metabolic pathways and cellular stress responses by modulating thioredoxin activity and ROS levels.

Pathways

TXNIP is a significant component of the oxidative stress and glucose metabolism pathways. In the oxidative stress pathway TXNIP interacts with thioredoxin influencing ROS detoxification and redox-sensitive signaling. In glucose metabolism TXNIP can affect insulin signaling by interacting with glucose transporter-1 (GLUT1). These interactions highlight TXNIP's regulatory role in cellular metabolism and stress-related signaling making it a node of convergence with proteins like thioredoxin and GLUT1.

TXNIP levels have been implicated in conditions such as type 2 diabetes and cardiovascular diseases. Elevated TXNIP expression has been observed in insulin resistance where it affects glucose uptake by modifying GLUT1 activity and links to thioredoxin in cardiovascular tissues resulting in oxidative stress damage. These connections position TXNIP as a potential therapeutic target for managing metabolic disorders and cardiovascular complications.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

May act as an oxidative stress mediator by inhibiting thioredoxin activity or by limiting its bioavailability (PubMed : 17603038). Interacts with COPS5 and restores COPS5-induced suppression of CDKN1B stability, blocking the COPS5-mediated translocation of CDKN1B from the nucleus to the cytoplasm (By similarity). Functions as a transcriptional repressor, possibly by acting as a bridge molecule between transcription factors and corepressor complexes, and over-expression will induce G0/G1 cell cycle arrest (PubMed : 12821938). Required for the maturation of natural killer cells (By similarity). Acts as a suppressor of tumor cell growth (PubMed : 18541147). Inhibits the proteasomal degradation of DDIT4, and thereby contributes to the inhibition of the mammalian target of rapamycin complex 1 (mTORC1) (PubMed : 21460850).
See full target information TXNIP
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Complementary Products

Assay Kits

AB83463

Thioredoxin Reductase Assay Kit (Colorimetric)

Functional Studies - Thioredoxin Reductase Assay Kit (Colorimetric) (AB83463)

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Proteins & Peptides

AB289756

Recombinant Human TXNIP protein

SDS-PAGE - Recombinant Human TXNIP protein (AB289756)

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Abcam product promise

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