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AB321547

Alexa Fluor® 750 Anti-TMEM175 抗体 [EPR24415-47]

Alexa Fluor® 750 Anti-TMEM175 antibody [EPR24415-47]

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Rabbit Recombinant Monoclonal TMEM175 antibody - conjugated to Alexa Fluor® 750.

別名を表示する

Endosomal/lysosomal proton channel TMEM175, Potassium channel TMEM175, Transmembrane protein 175, hTMEM175, TMEM175

関連する標識済み抗体及び組成の異なる製品 (6)

  • 578 PE

    PE Anti-TMEM175 antibody [EPR24415-47]

  • 660 APC

    APC Anti-TMEM175 antibody [EPR24415-47]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-TMEM175 antibody [EPR24415-47]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-TMEM175 antibody [EPR24415-47]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-TMEM175 antibody [EPR24415-47]

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-TMEM175 antibody [EPR24415-47]

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR24415-47

アイソタイプ

IgG

標識

Alexa Fluor® 750

励起波長/蛍光波長

Ex: 749nm, Em: 775nm

キャリアフリー

No

アプリケーション

Target Binding Affinity, Antibody Labelling

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

製品の詳細

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

How are conjugated primary antibodies validated?
This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle|Store in the dark

補足情報

This supplementary information is collated from multiple sources and compiled automatically.

TMEM175 also known as Transmembrane Protein 175 is a channel protein with dual function as a lysosomal potassium channel. It has an approximate mass of 66 kDa. TMEM175 plays a role in ion homeostasis and is expressed mainly in tissues with high levels of lysosomes including the brain and the immune system. This protein aids in the maintenance of the lysosomal environment important for cellular activities.
Biological function summary

This lysosomal protein contributes to the maintenance of ion stability within lysosomes an important factor in lysosomal degradation and overall cellular clearance processes. TMEM175 is an integral component of the lysosomal membrane and participates in ensuring lysosomal pH stability therefore influencing autophagic activity. TMEM175 does not participate in known protein complexes but it is pivotal for preserving lysosomal integrity.

Pathways

TMEM175 actively engages in the lysosomal degradation pathway impacting cellular waste processing. TMEM175 influences endolysosomal trafficking and autophagy which are essential for metabolite recycling. It works alongside proteins like ATPase and lysosomal enzyme hydrolases by controlling ion concentrations which can influence other lysosomal functions and pathways.

TMEM175 has a significant connection with neurodegenerative conditions such as Parkinson's disease. Mutation or dysfunction in TMEM175 impacts lysosomal function linked to protein aggregation often involving alpha-synuclein which serves as a hallmark for such neurodegenerative diseases. Additionally TMEM175 is implicated in lysosomal storage disorders where impaired lysosomal function results in cellular accumulation of substances due to ineffective degradation.

製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

ターゲットの情報

Proton-activated proton channel that catalyzes proton efflux from endosomes and lysosomes to maintain a steady-state pH (PubMed : 35333573, PubMed : 35750034, PubMed : 37390818). Activated at low pH (under pH 4.6) by luminal side protons : selectively mediates lysosomal proton release from lysosomes, eliciting a proton leak that balances V-ATPase activity to maintain pH homeostasis (PubMed : 35750034). Regulation of lumenal pH stability is required for autophagosome-lysosome fusion (PubMed : 26317472, PubMed : 32267231). Also acts as a potassium channel at higher pH, regulating potassium conductance in endosomes and lysosomes (PubMed : 26317472, PubMed : 28723891, PubMed : 32228865, PubMed : 32267231, PubMed : 33505021). Constitutes the pore-forming subunit of the lysoK(GF) complex, a complex activated by extracellular growth factors (PubMed : 33505021). The lysoK(GF) complex is composed of TMEM175 and AKT (AKT1, AKT2 or AKT3), a major target of growth factor receptors : in the complex, TMEM175 channel is opened by conformational changes by AKT, leading to its activation (PubMed : 33505021). The lysoK(GF) complex is required to protect neurons against stress-induced damage (PubMed : 33505021).
See full target information TMEM175

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