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AB321312

Alexa Fluor® 750 Anti-Spa-1 抗体 [EPR14134]

Alexa Fluor® 750 Anti-Spa-1 antibody [EPR14134]

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Rabbit Recombinant Monoclonal Spa-1 antibody - conjugated to Alexa Fluor® 750.

別名を表示する

SPA1, SIPA1, Signal-induced proliferation-associated protein 1, Sipa-1, GTPase-activating protein Spa-1, p130 SPA-1

関連する標識済み抗体及び組成の異なる製品 (6)

  • 578 PE

    PE Anti-Spa-1 antibody [EPR14134]

  • 660 APC

    APC Anti-Spa-1 antibody [EPR14134]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-Spa-1 antibody [EPR14134]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-Spa-1 antibody [EPR14134]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-Spa-1 antibody [EPR14134]

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-Spa-1 antibody [EPR14134]

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR14134

アイソタイプ

IgG

標識

Alexa Fluor® 750

励起波長/蛍光波長

Ex: 749nm, Em: 775nm

キャリアフリー

No

アプリケーション

Target Binding Affinity, Antibody Labelling

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

製品の詳細

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

How are conjugated primary antibodies validated?
This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle|Store in the dark

補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Spa-1 also known as human SPA plays a role in the regulation of small GTPase activity specifically targeting Rap1. This protein weighing approximately 120 kDa resides within various tissues with notable expression in the hematopoietic system and neuronal tissues. Researchers use names like Anti-SPA and Anti-Spa when referring to assays designed to recognize this protein. SPA-1 functions as a Rap1 GTPase-activating protein (GAP) facilitating the conversion of active GTP-bound Rap1 to its inactive GDP-bound form.
Biological function summary

Spa-1 engages in the control of cell adhesion and migration through its interaction with the Ras-related protein Rap1. As part of larger signal transduction complexes Spa-1 influences the organization of actin cytoskeleton and impacts integrin-mediated cell adhesion. Through its GAP activity it maintains cellular responsiveness to external stimuli impacting processes like immune cell trafficking and neuronal growth. Studies have noted its significance in maintaining homeostasis in tissues where it is expressed.

Pathways

Spa-1 integrates into key signaling cascades impacting the Ras superfamily of GTPases. Within the Rap1 signaling pathway Spa-1 works alongside proteins like RapGEF to finely regulate signal transduction processes affecting cell adhesion and cytoskeletal dynamics. In the context of the integrin signaling pathway Spa-1 modulates the actions of B-Raf in connection with Rap1 dictating responses necessary for cellular motility and positioning.

Spa-1 displays relevance to leukemia and neurological conditions. Its role in negative regulation of Rap1 activity links it to the aberrant proliferation seen in certain leukemia types where altered Rap1 signaling contributes to uncontrolled cell growth. Furthermore disruptions in Spa-1's modulation of neuronal Rap1 activity have associations with neurodegenerative diseases where proper cell migration and adhesion are impaired. Research explores its connections to proteins like BCR-ABL in the context of leukemia highlighting Spa-1 as a therapeutic target in disease management.

製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

ターゲットの情報

GTPase activator for the nuclear Ras-related regulatory proteins Rap1 and Rap2 in vitro, converting them to the putatively inactive GDP-bound state (PubMed : 9346962). Affects cell cycle progression (By similarity).
See full target information SIPA1

Abcam product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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