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AB321116

Alexa Fluor® 750 Anti-PRKAR1A+PRKAR1B 抗体 [EPR8491]

Alexa Fluor® 750 Anti-PRKAR1A+PRKAR1B antibody [EPR8491]

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Rabbit Recombinant Monoclonal PRKAR1A antibody - conjugated to Alexa Fluor® 750.

別名を表示する

PKR1, PRKAR1, TSE1, PRKAR1A, cAMP-dependent protein kinase type I-alpha regulatory subunit, Tissue-specific extinguisher 1

関連する標識済み抗体及び組成の異なる製品 (6)

  • 578 PE

    PE Anti-PRKAR1A+PRKAR1B antibody [EPR8491]

  • 660 APC

    APC Anti-PRKAR1A+PRKAR1B antibody [EPR8491]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-PRKAR1A+PRKAR1B antibody [EPR8491]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-PRKAR1A+PRKAR1B antibody [EPR8491]

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-PRKAR1A+PRKAR1B antibody [EPR8491]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-PRKAR1A+PRKAR1B antibody [EPR8491]

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR8491

アイソタイプ

IgG

標識

Alexa Fluor® 750

励起波長/蛍光波長

Ex: 749nm, Em: 775nm

キャリアフリー

No

アプリケーション

Antibody Labelling, Target Binding Affinity

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

製品の詳細

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

How are conjugated primary antibodies validated?
This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle|Store in the dark

補足情報

This supplementary information is collated from multiple sources and compiled automatically.

PRKAR1A and PRKAR1B also known as protein kinase A regulatory subunit 1 alpha and beta are key components of the cAMP-dependent protein kinase (PKA) complex. PRKAR1A has a mass of approximately 43 kDa while PRKAR1B mass closely resembles it. Both PRKAR1A and PRKAR1B are expressed in various tissues with PRKAR1A having a significant presence in heart and adrenal tissues and PRKAR1B more prominent in the brain. Their role involves regulating the location and activity of the catalytic subunits of PKA by binding cAMP which causes the release and activation of catalytic subunits.
Biological function summary

PRKAR1A and PRKAR1B participate in the regulation of PKA's activity which is important for cAMP signaling pathways. These proteins are part of the PKA holoenzyme complex functioning as inhibitory subunits. Upon binding cAMP they undergo a conformational change that releases PKA catalytic subunits allowing them to phosphorylate different substrates. This process affects cellular responses such as metabolism cell cycle progression and gene expression.

Pathways

The PKA signaling pathway is among the major pathways involving PRKAR1A and PRKAR1B. The pathway is linked to G protein-coupled receptors (GPCR) and the subsequent release of cAMP. Another important pathway is the MAPK/ERK pathway where PKA modulates the activity of certain MAPK-related proteins affecting cell division and differentiation. PRKAR1A and PRKAR1B are involved with other proteins like PRKACB and PRKACA which are catalytic subunits of PKA.

PRKAR1A mutations associate with Carney complex a disorder characterized by multiple neoplasms. This connection results from PRKAR1A gene inactivation that leads to dysregulation of cAMP signaling. PRKAR1B on the other hand relates to neuropsychiatric disorders like schizophrenia where altered PKA signaling affects neurotransmitter pathways. Both PRKAR1A and PRKAR1B's involvement with regulatory proteins like PP1 which interacts with PKA highlights their significance in disease pathogenesis.

製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

ターゲットの情報

Regulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells.
See full target information PRKAR1A

追加のターゲット

cAMP-dependent protein kinase type I-beta regulatory subunit

Abcam product promise

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