AB321241

Alexa Fluor® 750 Anti-NDUFAB1 抗体 [EPR6725]

Alexa Fluor® 750 Anti-NDUFAB1 antibody [EPR6725]

Recombinant
RabMAb
詳細を見る

Be the first to review this product! Submit a review

(0 Publication)

Rabbit Recombinant Monoclonal NDUFAB1 antibody - conjugated to Alexa Fluor® 750.

別名を表示する

ACP, CI-SDAP, NADH-ubiquinone oxidoreductase 9.6 kDa subunit, NDUFAB1

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR6725

アイソタイプ

IgG

標識

Alexa Fluor® 750

励起波長/蛍光波長

Ex: 749nm, Em: 775nm

キャリアフリー

アプリケーション

Antibody Labelling, Target Binding Affinity

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

製品の詳細

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

How are conjugated primary antibodies validated?
This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

style

left-column

出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle|Store in the dark

style

left-column

補足情報

This supplementary information is collated from multiple sources and compiled automatically.

NDUFAB1 also known as mitochondrial acyl-carrier protein is a small protein with a mass of approximately 16 kDa. It plays a role in the mitochondrial fatty acid synthesis pathway and the assembly of the electron transport chain complexes. It is ubiquitously expressed in various tissues indicating a significant role in cellular metabolism. The protein anchors to the matrix side of the inner mitochondrial membrane where it performs its mechanical functions.

Biological function summary

NDUFAB1 is a component of the mitochondrial respiratory chain specifically part of complex I also known as NADH:ubiquinone oxidoreductase. The protein binds to acyl groups and facilitates their transfer within mitochondrial processes. This function is important for the maintenance and regulation of energy production in cells. Its involvement in the synthesis of lipoic acid an essential cofactor for several mitochondrial enzyme complexes further highlights its biological significance.

Pathways

The complex I of which NDUFAB1 is a part participates in the oxidative phosphorylation pathway. This pathway is major in adenosine triphosphate (ATP) production which is critical for energy metabolism. NDUFAB1 closely interacts with NADH and other subunits within the complex to relay electrons through the mitochondrial membrane. This interaction is related to other proteins such as NDUFS6 and NDUFS7 contextualizing NDUFAB1 within a network integral for cellular respiration.

Research shows that mutations or dysregulation of NDUFAB1 can be associated with mitochondrial diseases such as Leigh syndrome a severe neurological disorder. The dysfunction of complex I in these diseases affects energy production which impacts high-energy demanding tissues. NDUFS1 is another protein within complex I that when mutated can also lead to similar disorders indicating that both proteins play roles in the development of mitochondrial dysfunctions.

style

left-column

style

left-column

製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

ターゲットの情報

Carrier of the growing fatty acid chain in fatty acid biosynthesis (By similarity) (PubMed : 27626371). Accessory and non-catalytic subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), which functions in the transfer of electrons from NADH to the respiratory chain (PubMed : 27626371). Accessory protein, of the core iron-sulfur cluster (ISC) assembly complex, that regulates, in association with LYRM4, the stability and the cysteine desulfurase activity of NFS1 and participates in the [2Fe-2S] clusters assembly on the scaffolding protein ISCU (PubMed : 31664822). The core iron-sulfur cluster (ISC) assembly complex is involved in the de novo synthesis of a [2Fe-2S] cluster, the first step of the mitochondrial iron-sulfur protein biogenesis. This process is initiated by the cysteine desulfurase complex (NFS1 : LYRM4 : NDUFAB1) that produces persulfide which is delivered on the scaffold protein ISCU in a FXN-dependent manner. Then this complex is stabilized by FDX2 which provides reducing equivalents to accomplish the [2Fe-2S] cluster assembly. Finally, the [2Fe-2S] cluster is transferred from ISCU to chaperone proteins, including HSCB, HSPA9 and GLRX5 (By similarity).

See full target information NDUFAB1

style

left-column

style

left-column

style

right-column

Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Alexa Fluor® 750 Anti-NDUFAB1 抗体 [EPR6725]

Alexa Fluor® 750 Anti-NDUFAB1 antibody [EPR6725]

関連する標識済み抗体及び組成の異なる製品 (8)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

標識

励起波長/蛍光波長

キャリアフリー

アプリケーション

免疫原

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

組合せ製品

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Abcam product promise