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AB321303

Alexa Fluor® 750 Anti-MED7 抗体 [EPR15410]

Alexa Fluor® 750 Anti-MED7 antibody [EPR15410]

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Rabbit Recombinant Monoclonal MED7 antibody - conjugated to Alexa Fluor® 750.

別名を表示する

ARC34, CRSP9, MED7, Mediator of RNA polymerase II transcription subunit 7, hMED7, Activator-recruited cofactor 34 kDa component, Cofactor required for Sp1 transcriptional activation subunit 9, Mediator complex subunit 7, RNA polymerase transcriptional regulation mediator subunit 7 homolog, Transcriptional coactivator CRSP33, CRSP complex subunit 9

関連する標識済み抗体及び組成の異なる製品 (10)

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR15410

アイソタイプ

IgG

標識

Alexa Fluor® 750

励起波長/蛍光波長

Ex: 749nm, Em: 775nm

キャリアフリー

No

アプリケーション

Target Binding Affinity, Antibody Labelling

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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製品の詳細

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

How are conjugated primary antibodies validated?
This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle|Store in the dark
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

MED7 also known as Mediator Complex Subunit 7 is a protein component of core Mediator complex essential for transcription regulation by RNA polymerase II. MED7 weighs approximately 25 kDa and exists in nucleus. It is part of a large multi-protein assembly. This subunit participates specifically by aiding in the mediator's interactions with gene-specific regulatory proteins and general transcription factors essential for transcription initiation.
Biological function summary

These components coordinate as part of Mediator complex which regulates transcription by acting as bridge between DNA-bound transcriptional regulators and RNA polymerase II. MED7 contributes to the structural integrity of Mediator complex affecting transcriptional activation processes necessary for gene expression. The protein's roles link it directly to cell growth differentiation and response to various stimuli indicating its importance in diverse cellular processes.

Pathways

MED7 integrates itself into transcription regulation pathways especially interacting with other subunits like MED1 and MED14. The Mediator complex functions in the pathway responsible for transferring information from transcription factors to RNA polymerase II. This involves interacting with multiple pathways including Notch signaling and Wnt signaling serving as coordinator between transcriptional regulators and transcription machinery.

MED7 has associations with cancer and neurodevelopmental disorders. Aberrations in Mediator complex components including MED7 can impact transcriptional dysregulation linked to oncogenesis. Mutations in this subunit along with changes in associated proteins such as MED12 are observed in various cancer types. Additionally disturbances in Mediator complex function potentially involving MED7 contribute to neurodevelopmental conditions due to misregulation of gene expression essential for neural development.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
See full target information MED7
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Abcam product promise

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