JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB275946

Alexa Fluor® 700 Anti-CD28 抗体 [CD28.2]

Alexa Fluor® 700 Anti-CD28 antibody [CD28.2]

Be the first to review this product! Submit a review

|

(0 Publication)

Anti-CD28 antibody [CD28.2] conjugated to Alexa Fluor® 700 - suitable for flow cytometry, and reacts with human samples. Clone [CD28.2] is a mouse monoclonal antibody and one of the most commonly used clones for flow cytometry.

Design your panel with our interactive flow cytometry panel builder. Click here.

別名を表示する

CD28, T-cell-specific surface glycoprotein CD28, TP44

2 Images
Flow Cytometry - Alexa Fluor® 700 Anti-CD28 antibody [CD28.2] (AB275946)
  • Flow Cyt

Unknown

Flow Cytometry - Alexa Fluor® 700 Anti-CD28 antibody [CD28.2] (AB275946)

Flow cytometry surface staining pattern of human peripheral whole blood stained using ab275946 (4 μl reagent / 100 μl of peripheral whole blood).

Flow Cytometry - Alexa Fluor® 700 Anti-CD28 antibody [CD28.2] (AB275946)
  • Flow Cyt

Unknown

Flow Cytometry - Alexa Fluor® 700 Anti-CD28 antibody [CD28.2] (AB275946)

Separation of CD28 positive lymphocytes (red-filled) from CD28 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using ab275946 (4 μl reagent / 100 μl of peripheral whole blood).

関連する標識済み抗体及び組成の異なる製品 (8)

  • 660 APC

    APC Anti-CD28 antibody [CD28.2]

  • 785 APC/Cy7®

    APC/Cy7® Anti-CD28 antibody [CD28.2]

  • Biotin

    Biotin Anti-CD28 antibody [CD28.2]

  • 519 FITC

    FITC Anti-CD28 antibody [CD28.2]

  • 578 PE

    PE Anti-CD28 antibody [CD28.2]

  • 778 PE/Cy7®

    PE/Cy7® Anti-CD28 antibody [CD28.2]

  • 675 PerCP

    PerCP Anti-CD28 antibody [CD28.2]

  • 695 PerCP/Cy5.5®

    PerCP/Cy5.5® Anti-CD28 antibody [CD28.2]

Key facts

宿主種

Mouse

クローン性

Monoclonal

クローン番号

CD28.2

アイソタイプ

IgG1

軽鎖のタイプ

kappa

標識

Alexa Fluor® 700

励起波長/蛍光波長

Ex: 702nm, Em: 723nm

キャリアフリー

No

交差種

Human

アプリケーション

Flow Cyt

applications

免疫原

Cell preparation containing CD28 protein. The exact immunogen used to generate this antibody is proprietary information.

P10747

style
left-column

組合せ製品

Assay Kits

AB223866

Human MDC ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human MDC ELISA Kit (AB223866)

  • 0
  • 0
Primary Antibodies

AB305048

Alexa Fluor® 647 Anti-CD8 alpha antibody [CAL66]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-CD8 alpha antibody [CAL66] (AB305048)

  • 5
  • 1
Primary Antibodies

AB196147

Alexa Fluor® 647 Anti-CD4 antibody [EPR6855]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-CD4 antibody [EPR6855] (AB196147)

  • 5
  • 1
Primary Antibodies

AB200315

Alexa Fluor® 488 Anti-CD45 antibody [EP322Y]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-CD45 antibody [EP322Y] (AB200315)

  • 0
  • 0
Primary Antibodies

AB307134

Alexa Fluor® 488 Anti-CD3 antibody [SP162]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-CD3 antibody [SP162] (AB307134)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p>4 μl reagent / 100 μl of whole blood or 10<sup>6</sup> cells in a suspension</p>" } } }
style
left-column

製品の詳細

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
style
left-column

出荷温度及び保存条件

製品の状態
Liquid
精製方法
Size-exclusion chromatography
バッファー組成
pH: 7.4 Preservative: 0.1% Sodium azide Constituents: PBS
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
+4°C
保管に関する情報
Do Not Freeze|Store in the dark
style
left-column

補足情報

This supplementary information is collated from multiple sources and compiled automatically.

CD28 also known as T-cell-specific surface glycoprotein CD28 or TP44 plays an important role as a co-stimulatory receptor on T cells. This glycoprotein has a molecular mass of about 44 kDa and is located mainly on the cell surface of T cells. CD28 expression also occurs on thymocytes and some B cells. It forms part of the immunoglobulin superfamily featuring in significant antibody interactions. Researchers often use anti-CD28 antibodies for various immunological assays including CD28 ELISA to study its function.
Biological function summary

CD28 co-stimulates T cells after antigen presentation. It enhances T cell proliferation survival and cytokine production. CD28 functions alongside the T cell receptor (TCR) complex mediating signals that promote full T cell activation. This interaction amplifies the immune response forming an important axis of immune system communication. CD28 interacts with its ligands B7-1 (CD80) and B7-2 (CD86) found on antigen-presenting cells (APCs) illustrating its role in immune system dynamics.

Pathways

CD28 is integral to the PI3K/Akt and MAPK/ERK signaling pathways. These pathways conduct signals critical for cellular growth and survival linking CD28 to downstream transcription factors. The interaction with another protein CTLA-4 acts as a regulatory balance for CD28 signaling preventing over-activation of T cells. This balance influences immune homeostasis and tolerance providing a framework for understanding costimulatory and inhibitory signaling.

CD28's role becomes evident in autoimmune diseases and transplant rejection. Abnormal CD28 signaling links to rheumatoid arthritis where inappropriate T cell activation occurs. Similarly CD28 activity contributes to organ transplant rejection by stimulating T cell-mediated responses against transplanted tissues. CTLA-4 through regulatory feedback can modify these effects providing therapeutic target strategies for managing such disorders. Efforts to modulate CD28 activity involve developing therapeutic agents such as anti-CD28 monoclonal antibodies.
style
left-column
style
left-column

製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

ターゲットの情報

Receptor that plays a role in T-cell activation, proliferation, survival and the maintenance of immune homeostasis (PubMed : 1650475, PubMed : 7568038). Functions not only as an amplifier of TCR signals but delivers unique signals that control intracellular biochemical events that alter the gene expression program of T-cells (PubMed : 24665965). Stimulation upon engagement of its cognate ligands CD80 or CD86 increases proliferation and expression of various cytokines in particular IL2 production in both CD4(+) and CD8(+) T-cell subsets (PubMed : 12196291, PubMed : 1650475, PubMed : 35397202). Mechanistically, ligation induces recruitment of protein kinase C-theta/PRKCQ and GRB2 leading to NF-kappa-B activation via both PI3K/Akt-dependent and -independent pathways (PubMed : 21964608, PubMed : 24665965, PubMed : 7568038). In conjunction with TCR/CD3 ligation and CD40L costimulation, enhances the production of IL4 and IL10 in T-cells (PubMed : 8617933).. Isoform 3. Enhances CD40L-mediated activation of NF-kappa-B and kinases MAPK8 and PAK2 in T-cells (PubMed : 15067037).
See full target information CD28
style
left-column
style
left-column
style
right-column

Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。
保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com