Alexa Fluor® 647 Anti-Visfatin 抗体 [EPR21980]

• Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-Visfatin antibody [EPR21980] (AB318236)

Flow cytometry scatter plots showing human peripheral mononuclear cells (PBMCs) stained with ab318236 (right) or isotype control (left). The cells were fixed and permeabilised using BD CytoFix/CytoPerm™ (20 min). The cells were incubated in 1x PBS containing 10μg/ml human IgG and 10% normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab318236) (1x 10⁶ in 100μl at 0.2 μg/ml (1/2500)) for 30min on ice. Alexa Fluor^® 647 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199093) (left). The cells were simultaneously stained with CD3.

Acquisition of >30000 events were collected using a 40 mW Red laser (638nm) and 660/10 bandpass filter.

This data was acquired using a Beckman Coulter CytoFlexLX flow cytometer.

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Visfatin antibody [EPR21980] (AB318236)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized K-562 (human chronic myelogenous leukemia lymphoblast) cells labelling Visfatin with ab318236 at 1/50 (10.0 ug/ml) dilution (Green).

Confocal image showing cytoplasmic and nuclear staining in K-562 cell line (shown in magenta). The counterstain was observed in green. Nuclear DNA was labeled with DAPI (shown in blue). Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

ab195887 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 488) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Visfatin antibody [EPR21980] (AB318236)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HCT 116 (human colorectal carcinoma epithelial cell) cells labelling Visfatin with ab318236 at 1/50 (10.0 ug/ml) dilution (Green).

Confocal image showing cytoplasmic and nuclear staining in HCT 116 cell line (shown in magenta). The counterstain was observed in green. Nuclear DNA was labeled with DAPI (shown in blue). Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

ab195887 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 488) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Visfatin antibody [EPR21980] (AB318236)

Immunohistochemical analysis of paraffin-embedded Human breast cancer tissue labeling Visfatin with ab318236 at 1/50 (10.0 ug/ml) dilution.

Positive staining on human breast cancer.
The section was incubated with ab318236 at 4°C overnight (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Counterstained with DAPI.

Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0)

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Visfatin antibody [EPR21980] (AB318236)

Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling Visfatin with ab318236 at 1/50 (10.0 ug/ml) dilution.

Positive staining on rat liver.
The section was incubated with ab318236 at 4°C overnight (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Counterstained with DAPI.

Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0)

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Visfatin antibody [EPR21980] (AB318236)

Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling Visfatin with ab318236 at 1/50 (10.0 ug/ml) dilution.

Positive staining on mouse kidney.
The section was incubated with ab318236 at 4°C overnight (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Counterstained with DAPI.

Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0)