JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB206887

Alexa Fluor® 647 Anti-VEGFA 抗体 [EP1176Y]

Alexa Fluor® 647 Anti-VEGFA antibody [EP1176Y]

Be the first to review this product! Submit a review

|

(4 Publications)

Rabbit Recombinant Monoclonal VEGFA antibody - conjugated to Alexa Fluor® 647. Suitable for ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse samples. Cited in 4 publications.

別名を表示する

VEGF, VEGFA, L-VEGF, Vascular permeability factor, VPF

1 Images
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-VEGFA antibody [EP1176Y] (AB206887)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-VEGFA antibody [EP1176Y] (AB206887)

ab206887 staining VEGF in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab206887 at 1/100 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

関連する標識済み抗体及び組成の異なる製品 (6)

  • 578 PE

    PE Anti-VEGFA antibody [EP1176Y]

  • Biotin

    Biotin Anti-VEGFA antibody [EP1176Y]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-VEGFA antibody [EP1176Y]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-VEGFA antibody [EP1176Y]

  • Unconjugated

    Anti-VEGFA antibody [EP1176Y] - C-terminal

  • Carrier free

    Anti-VEGFA antibody [EP1176Y] - BSA and Azide free

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EP1176Y

アイソタイプ

IgG

標識

Alexa Fluor® 647

励起波長/蛍光波長

Ex: 650nm, Em: 665nm

キャリアフリー

No

交差種

Human, Human, Mouse

アプリケーション

IHC-P, Flow Cyt (Intra), ICC/IF

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p>This product gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min).</p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
style
left-column

製品の詳細

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

style
left-column

出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle|Store in the dark
style
left-column

補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Vascular endothelial growth factor A (VEGFA) also known as VEGF or vascular permeability factor (VPF) is a protein that plays a central role in angiogenesis as well as in the growth of blood vessels. The molecular weight of VEGFA varies depending on the isoform typically ranging from 34 to 42 kDa. VEGFA is expressed in many tissues including fibroblasts macrophages endothelial cells and platelets. Its expression levels can increase in response to stimuli like hypoxia or inflammatory cytokines. VEGFA is an important target in research for therapies related to vascular diseases and cancer.
Biological function summary

VEGFA functions by promoting the proliferation and migration of vascular endothelial cells. It operates both as a homodimer and as part of complex signaling cascades interacting with VEGF receptors on cell surfaces to trigger downstream signals for angiogenesis. This includes endothelial cell growth migration and the new blood vessel formation process. VEGFA's activity is important for physiological processes such as wound healing and embryonic development and it also contributes to pathological conditions through its unregulated expression in diseases.

Pathways

Many processes involve VEGFA including the PI3K/AKT pathway and the MAPK/ERK pathway. These pathways regulate cell survival growth and differentiation. Other proteins like PLGF (placenta growth factor) and VEGFB often accompany VEGFA in these pathways aiding in distinct but overlapping roles. These interactions are transmitted through binding to VEGF receptors such as VEGFR-1 and VEGFR-2 subsequently activating various signaling cascades necessary for cellular and tissue homeostasis.

VEGFA relates to cancer and age-related macular degeneration among others. In cancer overexpression of VEGFA can lead to excessive angiogenesis supplying blood to tumors and facilitating tumor growth. In age-related macular degeneration upregulation of VEGFA contributes to the progression of the disease by promoting the formation of abnormal blood vessels under the retina. Anti-VEGFA therapies such as anti-VEGF antibodies (e.g. bevacizumab) can target these overexpressed pathways. VEGFA interacts with other proteins like HIF-1α which regulates its expression under hypoxia a common condition in tumors.
style
left-column
style
left-column

製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

ターゲットの情報

N-VEGF. Participates in the induction of key genes involved in the response to hypoxia and in the induction of angiogenesis such as HIF1A (PubMed : 35455969). Involved in protecting cells from hypoxia-mediated cell death (By similarity).. VEGFA. Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth (PubMed : 34530889). Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. Binds to the NRP1/neuropilin-1 receptor. Binding to NRP1 initiates a signaling pathway needed for motor neuron axon guidance and cell body migration, including for the caudal migration of facial motor neurons from rhombomere 4 to rhombomere 6 during embryonic development (By similarity). Also binds the DEAR/FBXW7-AS1 receptor (PubMed : 17446437).. Isoform VEGF165B. Binds to the KDR receptor but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth.
See full target information VEGFA
style
left-column

文献 (4)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 24: PubMed37958946

2023

Melatonin Exerts Prominent, Differential Epidermal and Dermal Anti-Aging Properties in Aged Human Eyelid Skin Ex Vivo.

Applications

Unspecified application

Species

Unspecified reactive species

Tara Samra,Tatiana Gomez-Gomez,Kinga Linowiecka,Aysun Akhundlu,Gabriella Lopez de Mendoza,Matthew Gompels,Wendy W Lee,Jennifer Gherardini,Jérémy Chéret,Ralf Paus

Bioactive materials 12:71-84 PubMed35087964

2022

Spatiotemporally controlled, aptamers-mediated growth factor release locally manipulates microvasculature formation within engineered tissues.

Applications

Unspecified application

Species

Unspecified reactive species

Deepti Rana,Ajoy Kandar,Nasim Salehi-Nik,Ilyas Inci,Bart Koopman,Jeroen Rouwkema

Journal of materials science. Materials in medicine 33:4 PubMed34940936

2021

Hydrogel contained valproic acid accelerates bone-defect repair via activating Notch signaling pathway in ovariectomized rats.

Applications

Unspecified application

Species

Unspecified reactive species

Zhou-Shan Tao,Tian-Lin Li,Hong-Guang Xu,Min Yang

PloS one 16:e0247492 PubMed33690618

2021

Anti-cancer effects of polyphenol-rich sugarcane extract.

Applications

Unspecified application

Species

Unspecified reactive species

Monica D Prakash,Lily Stojanovska,Jack Feehan,Kulmira Nurgali,Elizabeth L Donald,Magdalena Plebanski,Matthew Flavel,Barry Kitchen,Vasso Apostolopoulos
View all publications
style
left-column
style
left-column
style
right-column

代替製品

Primary Antibodies

AB83132

Biotin Anti-VEGFA antibody

Sandwich ELISA - Biotin Anti-VEGFA antibody (AB83132)

  • 0
  • 0
Primary Antibodies

AB209835

Anti-VEGFA antibody

Lab Essentials

IHC - Wholemount - Anti-VEGFA antibody (AB209835)

  • 0
  • 0
Primary Antibodies

AB183100

Anti-VEGFA antibody

Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VEGFA antibody (AB183100)

  • 1
  • 1
Primary Antibodies

AB155944

Anti-VEGFA antibody [16F1]

Lab Essentials

Immunocytochemistry - Anti-VEGFA antibody [16F1] (AB155944)

  • 0
  • 0
Primary Antibodies

AB1316

Anti-VEGFA antibody [VG-1]

BOND RX™ Validated|Lab Essentials|Trial Size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VEGFA antibody [VG-1] (AB1316)

  • 4
  • 45
Primary Antibodies

AB46154

Anti-VEGFA antibody

Lab Essentials

Western blot - Anti-VEGFA antibody (AB46154)

  • 5
  • 29
Primary Antibodies

AB27620

Anti-VEGFA antibody [SP28], prediluted

RabMAb|Recombinant|Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VEGFA antibody [SP28], prediluted (AB27620)

  • 4
  • 2
Primary Antibodies

AB213244

Anti-VEGFA antibody [EPR21219]

BOND RX™ Validated|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VEGFA antibody [EPR21219] (AB213244)

  • 0
  • 2
Primary Antibodies

AB39250

Anti-VEGFA antibody

Immunocytochemistry/ Immunofluorescence - Anti-VEGFA antibody (AB39250)

  • 4
  • 1
Primary Antibodies

AB229377

Anti-VEGFA antibody [EPR20705] - BSA and Azide free

RabMAb|Recombinant

Western blot - Anti-VEGFA antibody [EPR20705] - BSA and Azide free (AB229377)

  • 0
  • 0
Primary Antibodies

AB214424

Anti-VEGFA antibody [EPR20705]

RabMAb|Recombinant|Lab Essentials|20ul selling size

Western blot - Anti-VEGFA antibody [EPR20705] (AB214424)

  • 0
  • 0

組合せ製品

Assay Kits

AB222510

Human VEGF ELISA Kit

SimpleStep

ELISA - Human VEGF ELISA Kit (AB222510)

  • 5
  • 1
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB195028

Alexa Fluor® 488 Anti-Von Willebrand Factor antibody [EPSISR15]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Von Willebrand Factor antibody [EPSISR15] (AB195028)

  • 5
  • 1
Primary Antibodies

AB9485

Anti-GAPDH antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (AB9485)

  • 5
  • 88
Primary Antibodies

AB15580

Anti-Ki67 antibody

BOND RX™ Validated|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody (AB15580)

  • 5
  • 230

Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。
保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com