Alexa Fluor® 647 Anti-TNF-alpha 抗体 [EPR21753-109] (ab300093)
![Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-TNF-alpha antibody [EPR21753-109] (AB300093)](https://www.abcam.co.jp/ps/products/300/ab300093/Images/AB300093-486116-anti-TNFalpha-EPR21753109-flow-cytometry-RAW-2647.jpg "Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-TNF-alpha antibody [EPR21753-109] (AB300093)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Alexa Fluor® 647 Rabbit monoclonal [EPR21753-109] to TNF alpha
- Suitable for: ICC/IF, Flow Cyt (Intra)
- Reacts with: Mouse
- Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm
Related conjugates and formulations
製品の概要
-
製品名
Alexa Fluor® 647 Anti-TNF-alpha antibody [EPR21753-109]
TNF alpha 一次抗体 製品一覧 -
製品の詳細
Alexa Fluor® 647 Rabbit monoclonal [EPR21753-109] to TNF alpha -
由来種
Rabbit -
標識
Alexa Fluor® 647. Ex: 652nm, Em: 668nm -
特異性
The protein level of TNF alpha in normal samples is very weak. The TNF alpha expression must be stimulated.
-
アプリケーション
適用あり: ICC/IF, Flow Cyt (Intra)more details -
種交差性
交差種: Mouse -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
-
ポジティブ･コントロール
- ICC: RAW 264.7 (mouse abelson murine leukemia virus-induced tumor macrophages) Flow Cyt (Intra): RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophages) treated with 100ng/ml LPS
-
特記事項
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 30% Glycerol (glycerin, glycerine), 68% PBS, 1% BSA -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR21753-109 -
アイソタイプ
IgG -
研究分野
関連製品
-
Alternative Versions
-
Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab300093の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF |
1/100.
|
|
| Flow Cyt (Intra) |
1/50.
|
| 特記事項 |
|---|
|
ICC/IF
1/100. |
|
Flow Cyt (Intra)
1/50. |
ターゲット情報
-
機能
Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. -
関連疾患
Genetic variations in TNF are a cause of susceptibility psoriatic arthritis (PSORAS) [MIM:607507]. PSORAS is an inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy. Five types of psoriatic arthritis have been defined: asymmetrical oligoarthritis characterized by primary involvement of the small joints of the fingers or toes; asymmetrical arthritis which involves the joints of the extremities; symmetrical polyarthritis characterized by a rheumatoidlike pattern that can involve hands, wrists, ankles, and feet; arthritis mutilans, which is a rare but deforming and destructive condition; arthritis of the sacroiliac joints and spine (psoriatic spondylitis). -
配列類似性
Belongs to the tumor necrosis factor family. -
翻訳後修飾
The soluble form derives from the membrane form by proteolytic processing.
The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1.
O-glycosylated; glycans contain galactose, N-acetylgalactosamine and N-acetylneuraminic acid. -
細胞内局在
Secreted and Cell membrane. - Information by UniProt
-
参照データベース
- Entrez Gene: 21926 Mouse
- SwissProt: P06804 Mouse
- Unigene: 1293 Mouse
-
別名
- APC1 antibody
- APC1 protein antibody
- Cachectin antibody
see all
画像
-
Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-TNF-alpha antibody [EPR21753-109] (AB300093)
Intracellular Flow Cytometry analysis of RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophages) treated with 100ng/ml LPS for 3 hours, then together with 300ng/ml BFA for another 3 hours labelling TNF alpha (Red) with ab300093 at a 1/100 dilution. Untreated control (Green). Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. Rabbit monoclonal isotype control - Alexa Fluor® 647 (Black). Unlabelled control - Cells without incubation with primary and secondary antibodies (Blue).
-
![Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-TNF-alpha antibody [EPR21753-109] (ab300093)](https://www.abcam.co.jp/ps/products/300/ab300093/Images/AB300093-486115-anti-TNFalpha-EPR21753109-ICC-RAW-2647.jpg)
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-TNF-alpha antibody [EPR21753-109] (ab300093)Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized RAW 264.7 (mouse abelson murine leukemia virus-induced tumor macrophages) labelling TNF alpha with ab300093 at 1/100 dilution (red). Confocal image showing cytoplasmic staining in RAW 264.7 cell line treated with 100ng/ml LPS for 3h, then together with 300ng/ml BFA for another 3h. The nuclear counterstain is DAPI (blue).
Tubulin is detected with ab195887 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 488) (green) at 1/200 dilution.
-
![Alexa Fluor® 647 Anti-TNF-alpha antibody [EPR21753-109] (AB300093)](https://www.abcam.co.jp/ps/products/300/ab300093/Images/AB300093-486738-Benefits-of-Recombinant-antibodies.jpg)
Alexa Fluor® 647 Anti-TNF-alpha antibody [EPR21753-109] (AB300093)
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (1)
ab300093 は 1 報の論文で使用されています。
- Hao Y et al. Preclinical evaluation of the safety and effectiveness of a new bioartificial cornea. Bioact Mater 29:265-278 (2023). PubMed: 37600931