Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
リコンビナントRabMAb

Alexa Fluor® 647 Anti-TMS1/ASC 抗体 [EPR23978-28] (ab300732)

Submit a review Submit a question
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-TMS1/ASC antibody [EPR23978-28] (AB300732)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-TMS1/ASC antibody [EPR23978-28] (AB300732)
  • Alexa Fluor® 647 Anti-TMS1/ASC antibody [EPR23978-28] (AB300732)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Alexa Fluor® 647 Rabbit monoclonal [EPR23978-28] to TMS1/ASC
  • Suitable for: IHC-P
  • Reacts with: Human
  • Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm

Conjugates logo Related conjugates and formulations

Carrier Free Unconjugated

製品の概要

  • 製品名

    Alexa Fluor® 647 Anti-TMS1/ASC antibody [EPR23978-28]
    TMS1/ASC 一次抗体 製品一覧

  • 製品の詳細

    Alexa Fluor® 647 Rabbit monoclonal [EPR23978-28] to TMS1/ASC

  • 由来種

    Rabbit

  • 標識

    Alexa Fluor® 647. Ex: 652nm, Em: 668nm

  • アプリケーション

    適用あり: IHC-Pmore details

  • 種交差性

    交差種: Human

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • IHC-P: Human tonsil and human colon carcinoma.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab300732の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-P
1/100.

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0)
特記事項
IHC-P
1/100.

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0)

ターゲット情報

  • 機能

    Promotes caspase-mediated apoptosis. This proapoptotic activity is mediated predominantly through the activation of caspase-9. May be a component of the inflammasome, a protein complex which also includes NALP2, CARD8 and CASP1 and whose function would be the activation of proinflammatory caspases.

  • 組織特異性

    Widely expressed at low levels. Detected in peripheral blood leukocytes, lung, small intestine, spleen, thymus, colon and at lower levels in placenta, liver and kidney. Very low expression in skeletal muscle, heart and brain. Detected in the leukemia cell lines HL-60 and U937, but not in Jurkat T-cell lymphoma and Daudi Burkitt's lymphoma. Detected in the melanoma cell line WM35, but not in WM793. Not detected in HeLa cervical carcinoma cells and Molt 4 lymphocytic leukemia cells.

  • 配列類似性

    Contains 1 CARD domain.
    Contains 1 DAPIN domain.

  • ドメイン

    Interacts with CIAS1/PYPAF1 and PYDC1 via the DAPIN domain.

  • 翻訳後修飾

    Phosphorylated.

  • 細胞内局在

    Cytoplasm. Upstream of caspase activation, a redistribution from the cytoplasm to the aggregates occurs. These appear as hollow, perinuclear spherical, ball-like structures.
  • Information by UniProt

  • 参照データベース

    • 別名

      • Apoptosis associated speck like protein containing a CARD antibody
      • Apoptosis-associated speck-like protein containing a CARD antibody
      • ASC antibody
      • ASC_HUMAN antibody
      • CARD 5 antibody
      • CARD5 antibody
      • Caspase recruitment domain containing protein 5 antibody
      • Caspase recruitment domain protein 5 antibody
      • Caspase recruitment domain-containing protein 5 antibody
      • hASC antibody
      • MGC10332 antibody
      • PYCARD antibody
      • PYD and CARD domain containing antibody
      • PYD and CARD domain containing protein antibody
      • PYD and CARD domain-containing protein antibody
      • Target of methylation induced silencing 1 antibody
      • Target of methylation-induced silencing 1 antibody
      • TMS 1 antibody
      • TMS antibody
      • TMS1 antibody
      see all

    画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-TMS1/ASC antibody [EPR23978-28] (AB300732)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-TMS1/ASC antibody [EPR23978-28] (AB300732)

      Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling TMS1/ASC with ab300732 at 1/100 (5.0 µg/ml). Positive staining on human tonsil is observed. The tissue section was first incubated with TBS containing 0.025% (v/v) Triton X-100, 0.3 M glycine and 1% (w/v) BSA for 1 h at room temperature to block non-specific protein-protein interactions, followed by overnight incubation at +4°C with ab300732 at 1/100 dilution in TBS containing 0.025% (v/v) Triton X-100 and 1% (w/v) BSA (shown in red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.  Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

      Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). 

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-TMS1/ASC antibody [EPR23978-28] (AB300732)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-TMS1/ASC antibody [EPR23978-28] (AB300732)

      Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue labeling TMS1/ASC with ab300732 at 1/100 (5.0 µg/ml). Positive staining on human colon carcinoma is observed.

      The tissue section was first incubated with TBS containing 0.025% (v/v) Triton X-100, 0.3 M glycine and 1% (w/v) BSA for 1 h at room temperature to block non-specific protein-protein interactions, followed by overnight incubation at +4°C with ab300732 at 1/100 dilution in TBS containing 0.025% (v/v) Triton X-100 and 1% (w/v) BSA (shown in red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.  Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

      Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). 

    • Alexa Fluor® 647 Anti-TMS1/ASC antibody [EPR23978-28] (AB300732)
      Alexa Fluor® 647 Anti-TMS1/ASC antibody [EPR23978-28] (AB300732)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab300732 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab300732 は論文での使用が確認できていません。

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    There are currently no Customer reviews or Questions for ab300732.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com