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AB310129

Alexa Fluor® 647 Anti-Sonic Hedgehog 抗体 [EP1190Y]

Alexa Fluor® 647 Anti-Sonic Hedgehog antibody [EP1190Y]

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Rabbit Recombinant Monoclonal Sonic Hedgehog antibody - conjugated to Alexa Fluor® 647. Suitable for Target Binding Affinity, Antibody Labelling, Flow Cyt (Intra), ICC/IF, IHC-P and reacts with Human samples.

別名を表示する

Sonic hedgehog protein, SHH, HHG-1, Shh unprocessed N-terminal signaling and C-terminal autoprocessing domains, ShhNC

関連する標識済み抗体及び組成の異なる製品 (8)

  • Unconjugated

    Anti-Sonic Hedgehog antibody [EP1190Y]

  • Carrier free

    Anti-Sonic Hedgehog antibody [EP1190Y] - BSA and Azide free

  • 660 APC

    APC Anti-Sonic Hedgehog antibody [EP1190Y]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-Sonic Hedgehog antibody [EP1190Y]

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-Sonic Hedgehog antibody [EP1190Y]

  • 603 Alexa Fluor® 568

    Alexa Fluor® 568 Anti-Sonic Hedgehog antibody [EP1190Y]

  • HRP

    HRP Anti-Sonic Hedgehog antibody [EP1190Y]

  • 578 PE

    PE Anti-Sonic Hedgehog antibody [EP1190Y]

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EP1190Y

アイソタイプ

IgG

標識

Alexa Fluor® 647

励起波長/蛍光波長

Ex: 650nm, Em: 665nm

キャリアフリー

No

交差種

Human

アプリケーション

Antibody Labelling, ICC/IF, Flow Cyt (Intra), Target Binding Affinity, IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

特異性

<p>Specific for both full length (51kDa) and c-product subunit (27kDa) of human Sonic Hedgehog protein</p>

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組合せ製品

Assay Kits

AB100639

Human Sonic Hedgehog ELISA Kit

Sandwich ELISA - Human Sonic Hedgehog ELISA Kit (AB100639)

  • 4
  • 1
Primary Antibodies

AB199471

Alexa Fluor® 647 Anti-NDRG1 antibody [EPR5593]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-NDRG1 antibody [EPR5593] (AB199471)

  • 0
  • 0
Primary Antibodies

AB190569

Alexa Fluor® 647 Anti-HIF-1 alpha antibody [EP1215Y]

RabMAb|Recombinant|Trial Size

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-HIF-1 alpha antibody [EP1215Y] (AB190569)

  • 0
  • 0
Primary Antibodies

AB281580

Alexa Fluor® 647 Anti-Indoleamine 2, 3-dioxygenase antibody [EPR20374]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Indoleamine 2, 3-dioxygenase antibody [EPR20374] (AB281580)

  • 0
  • 0
Primary Antibodies

AB307855

Alexa Fluor® 488 Anti-PDGFR alpha antibody [EPR22059-270]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-PDGFR alpha antibody [EPR22059-270] (AB307855)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "TargetBindingAffinity" : {"fullname" : "Target Binding Affinity", "shortname":"Target Binding Affinity"}, "AntibodyLabelling" : {"fullname" : "Antibody Labelling", "shortname":"Antibody Labelling"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "TargetBindingAffinity-species-checked": "guaranteed", "TargetBindingAffinity-species-dilution-info": "", "TargetBindingAffinity-species-notes": "", "AntibodyLabelling-species-checked": "guaranteed", "AntibodyLabelling-species-dilution-info": "", "AntibodyLabelling-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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製品の詳細

How are conjugated primary antibodies validated?
This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle|Store in the dark
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Sonic Hedgehog also known as SHH is a protein that plays an important role in cell signaling during embryonic development. It has alternative names like 'HHG-1' and '5h4'. The Sonic Hedgehog protein is about 45 kDa in mass. Scientists find SHH expression in various tissues including the brain lungs and limb buds. Its activity plays a role in the organization of several tissue structures during development helping to regulate cellular growth and differentiation processes.
Biological function summary

Sonic Hedgehog is critical for the vertebrate embryonic patterning and organogenesis. It functions as a signaling molecule and is part of the Hedgehog signaling complex. The protein acts by binding to the Patched receptor further influencing the Smoothened transducer in cellular signaling cascades. This signaling contributes to the proper formation of limbs central nervous system structures and other vital organs in early development stages.

Pathways

Sonic Hedgehog participates in essential signaling pathways such as the Hedgehog pathway and the Wnt signaling pathway. It interacts with proteins including the Patched and Smoothened to facilitate downstream genetic transcription regulation. Through these pathways Sonic Hedgehog influences the transcription of specific genes that control cell growth. These interactions contribute to the regulation of different cell types and the organization of distinct tissue areas.

Sonic Hedgehog connects to issues like cancer and congenital malformations. Aberrations in its signaling can lead to conditions such as basal cell carcinoma and holoprosencephaly. Sonic Hedgehog through its influence on the Hedgehog signaling pathway implicates interactions with other proteins like GLI transcription factors and Patched. These interactions can dysregulate cellular processes and result in disease states when not functioning correctly.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Sonic hedgehog protein. The C-terminal part of the sonic hedgehog protein precursor displays an autoproteolysis and a cholesterol transferase activity (By similarity). Both activities result in the cleavage of the full-length protein into two parts (ShhN and ShhC) followed by the covalent attachment of a cholesterol moiety to the C-terminal of the newly generated ShhN (By similarity). Both activities occur in the endoplasmic reticulum (By similarity). Once cleaved, ShhC is degraded in the endoplasmic reticulum (By similarity).. Sonic hedgehog protein N-product. The dually lipidated sonic hedgehog protein N-product (ShhNp) is a morphogen which is essential for a variety of patterning events during development. Induces ventral cell fate in the neural tube and somites (PubMed : 24863049). Involved in the patterning of the anterior-posterior axis of the developing limb bud (By similarity). Essential for axon guidance (By similarity). Binds to the patched (PTCH1) receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes (PubMed : 10753901). In the absence of SHH, PTCH1 represses the constitutive signaling activity of SMO (PubMed : 10753901).
See full target information SHH
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Abcam product promise

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