Alexa Fluor® 647 Anti-Scn1a 抗体 [EPR22326-112] (ab288363)

Immunofluorescence staining of Scn1a in a section of frozen mouse normal brain.

The section was fixed using 10% formaldehyde in PBS for 10 minutes. No antigen retrieval step was performed prior to staining. Performed on a Leica BOND^®. The section was incubated at room temperature for 1 hour with ab288363 at 1/50 dilution (shown in red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Dako Fluorescence Mounting Medium^®.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

Negative immunofluorescence staining of Scn1a in a section of frozen mouse normal heart.

The section was fixed using 10% formaldehyde in PBS for 10 minutes. No antigen retrieval step was performed prior to staining. Performed on a Leica BOND^{®<\sup>. The section was incubated at room temperature for 1 hour with ab288363 at 1/50 dilution (shown in red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Dako Fluorescence Mounting Medium ®.}

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

Immunofluorescence staining of Scn1a in a section of formalin-fixed paraffin-embedded mouse normal brain.

Performed on a Leica BOND^{®<\sup>. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH9.0) using retrieval settings of 110°C for 40 minutes. The section was then incubated at room temperature for 1 hour with ab288363 at 1/100 dilution (shown in red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Dako Fluorescence Mounting Medium ®.}

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

Negative immunofluorescence staining of Scn1a in a section of formalin-fixed paraffin-embedded mouse normal heart.

Performed on a Leica BOND^{®<\sup>. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH9.0) using retrieval settings of 110°C for 40 minutes. The section was then incubated at room temperature for 1 hour with ab288363 at 1/100 dilution (shown in red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Dako Fluorescence Mounting Medium ®.}

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

Immunofluorescent staining of Scn1a in a section of formalin-fixed paraffin-embedded mouse normal brain.

Performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH9.0) using retrieval settings of 110°C for 40 minutes. The section was then incubated at room temperature for 1 hour with ab288363 at 1/100 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Dako Fluorescence Mounting Medium ^®.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

Immunofluorescent staining of Scn1a in a section of frozen mouse normal brain.

Performed on a Leica BOND. The section was incubated at room temperature for 1 hour with ab288363 at 1/50 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Dako Fluorescence Mounting Medium ^®.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.