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リコンビナントRabMAb

Alexa Fluor® 647 Anti-SARS CoV-2 ORF3a Protein 抗体 [EPR24848-9] (ab300675)

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-SARS CoV-2 ORF3a Protein antibody [EPR24848-9] (ab300675)
  • Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-SARS CoV-2 ORF3a Protein antibody [EPR24848-9] (AB300675)
  • Alexa Fluor® 647 Anti-SARS CoV-2 ORF3a Protein antibody [EPR24848-9] (AB300675)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Alexa Fluor® 647 Rabbit monoclonal [EPR24848-9] to SARS CoV-2 ORF3a Protein
  • Suitable for: Flow Cyt (Intra), ICC/IF
  • Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Carrier Free Unconjugated

製品の概要

  • 製品名

    Alexa Fluor® 647 Anti-SARS CoV-2 ORF3a Protein antibody [EPR24848-9]
    SARS CoV-2 ORF3a Protein 一次抗体 製品一覧

  • 製品の詳細

    Alexa Fluor® 647 Rabbit monoclonal [EPR24848-9] to SARS CoV-2 ORF3a Protein

  • 由来種

    Rabbit

  • 標識

    Alexa Fluor® 647. Ex: 652nm, Em: 668nm

  • アプリケーション

    適用あり: Flow Cyt (Intra), ICC/IFmore details

  • 種交差性

    交差種: SARS-CoV-2

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • ICC/IF: HEK-293T (Human embryonic kidney epithelial cell) transfected with Myc tagged SARS-CoV-2 Protein 3a construct. Flow cyt. Int.: HEK-293T (Human embryonic kidney epithelial cell) transfected with myc-tagged SARS-CoV-2 Protein 3a expression construct.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark.

  • バッファー

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPR24848-9

  • アイソタイプ

    IgG

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab300675の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt (Intra)
1/500.
ICC/IF
1/100.
特記事項
Flow Cyt (Intra)
1/500.
ICC/IF
1/100.

ターゲット情報

  • 関連性

    ORF3a is the largest accessory protein in SARS-CoV-2 virus, containing 274 amino acids. ORF3a is involved in viral entry within the host and has immunogenic properties. Moreover, it is involved in pro-inflammatory cytokine and chemokine production by activating various signal transduction pathways, leading to cytokine storm Covid-19 patients. ORF3a is also implicated in ion channel formation and modulates release of virus from the host cell.

  • 別名

    • SARS CoV-2 Protein 3a antibody

画像

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-SARS CoV-2 ORF3a Protein antibody [EPR24848-9] (ab300675)
    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-SARS CoV-2 ORF3a Protein antibody [EPR24848-9] (ab300675)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HEK-293T (Human embryonic kidney epithelial cell) transfected with Myc tagged SARS-CoV-2 Protein 3a construct cells labelling SARS CoV-2 ORF3a Protein with ab300675 at 1/100 dilution (5.0 µg/mL) (Red). Confocal image showing cytoplasmic staining in HEK-293T cells transfected with myc-tagged SARS-CoV-2 Protein 3a expression vector, and no staining in HEK-293T cells transfected with myc-tagged SARS-COV-1 Protein 3a expression vector. Myc-Tag Mouse mAb (Alexa Fluor® 488) was used to counterstain tubulin at 1/100 dilution (0.5 µg/mL) (Green). The Nuclear counterstain was DAPI (Blue).

     

  • Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-SARS CoV-2 ORF3a Protein antibody [EPR24848-9] (AB300675)
    Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-SARS CoV-2 ORF3a Protein antibody [EPR24848-9] (AB300675)

    Flow cytometric (Intracellular) analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HEK-293T (Human embryonic kidney epithelial cell) transfected with myc-tagged SARS-COV-1 Protein 3a expression construct (Left)/ HEK-293T (Human embryonic kidney epithelial cell) transfected with myc-tagged SARS-CoV-2 Protein 3a expression construct (Right) cells labeling SARS CoV-2 ORF3a Protein with ab300675 at 1/500 dilution (0.1 µg) (Red) compared with a isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue).

  • Alexa Fluor® 647 Anti-SARS CoV-2 ORF3a Protein antibody [EPR24848-9] (AB300675)
    Alexa Fluor® 647 Anti-SARS CoV-2 ORF3a Protein antibody [EPR24848-9] (AB300675)

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (0)

ab300675 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab300675 は論文での使用が確認できていません。

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