Alexa Fluor® 647 Anti-RBBP7 抗体 [EPR23796-74] (ab286823)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-RBBP7 antibody [EPR23796-74] (ab286823)](https://www.abcam.co.jp/ps/products/286/ab286823/Images/ab286823-1-IHCAP00479841.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-RBBP7 antibody [EPR23796-74] (ab286823)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Alexa Fluor® 647 Rabbit monoclonal [EPR23796-74] to RBBP7
- Suitable for: IHC-P, ICC
- Knockout validated
- Reacts with: Human
- Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm
Related conjugates and formulations
製品の概要
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製品名
Alexa Fluor® 647 Anti-RBBP7 antibody [EPR23796-74]
RBBP7 一次抗体 製品一覧 -
製品の詳細
Alexa Fluor® 647 Rabbit monoclonal [EPR23796-74] to RBBP7 -
由来種
Rabbit -
標識
Alexa Fluor® 647. Ex: 652nm, Em: 668nm -
アプリケーション
適用あり: IHC-P, ICCmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- IHC-P: human lung cancer. ICC: Wild-type Hap1 cells
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特記事項
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark. -
バッファー
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, 68% PBS -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR23796-74 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
- Anti-RBBP7 antibody [EPR23796-74] - ChIP Grade - BSA and Azide free (ab273882)
- Alexa Fluor® 488 Anti-RBBP7 antibody [EPR23796-74] (ab290984)
- AP Anti-RBBP7 antibody [EPR23796-74] - ChIP Grade (ab308651)
- Alexa Fluor® 594 Anti-RBBP7 antibody [EPR23796-74] - ChIP Grade (ab310352)
- Alexa Fluor® 555 Anti-RBBP7 antibody [EPR23796-74] - ChIP Grade (ab311874)
- Alexa Fluor® 568 Anti-RBBP7 antibody [EPR23796-74] - ChIP Grade (ab312343)
- Alexa Fluor® 750 Anti-RBBP7 antibody [EPR23796-74] - ChIP Grade (ab321518)
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab286823の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P |
1/100.
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| ICC |
Use a concentration of 1 µg/ml.
|
| 特記事項 |
|---|
|
IHC-P
1/100. |
|
ICC
Use a concentration of 1 µg/ml. |
ターゲット情報
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機能
Core histone-binding subunit that may target chromatin remodeling factors, histone acetyltransferases and histone deacetylases to their histone substrates in a manner that is regulated by nucleosomal DNA. Component of several complexes which regulate chromatin metabolism. These include the type B histone acetyltransferase (HAT) complex, which is required for chromatin assembly following DNA replication; the core histone deacetylase (HDAC) complex, which promotes histone deacetylation and consequent transcriptional repression; the nucleosome remodeling and histone deacetylase complex (the NuRD complex), which promotes transcriptional repression by histone deacetylation and nucleosome remodeling; and the PRC2/EED-EZH2 complex, which promotes repression of homeotic genes during development; and the NURF (nucleosome remodeling factor) complex. -
配列類似性
Belongs to the WD repeat RBAP46/RBAP48/MSI1 family.
Contains 7 WD repeats. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 5931 Human
- Omim: 300825 Human
- SwissProt: Q16576 Human
- Unigene: 495755 Human
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別名
- G1/S transition control protein binding protein RbAp46 antibody
- Histone acetyltransferase type B subunit 2 antibody
- Histone binding protein RBBP7 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-RBBP7 antibody [EPR23796-74] (ab286823)
Immunofluorescent staining of RbAp46 in a section of formalin-fixed paraffin-embedded human lung cancer*.
Performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH9.0) using retrieval settings of 110°C for 40 minutes. The section was then incubated at room temperature for 1 hour with ab286823 at 1/100 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Dako Fluorescence Mounting Medium ®.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.
*Tissue obtained from Papworth Hospital Research Tissue Bank.
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![Immunocytochemistry - Alexa Fluor® 647 Anti-RBBP7 antibody [EPR23796-74] (ab286823)](https://www.abcam.co.jp/ps/products/286/ab286823/Images/ab286823-2-anti-RBBP7-A647-Human-Hap1RBBP7-ICC.jpg)
Immunocytochemistry - Alexa Fluor® 647 Anti-RBBP7 antibody [EPR23796-74] (ab286823)ab286823 staining RbAp46 in wild-type Hap1 cells, with negative expression in RBBP7 knockout Hap1 cells. The cells were fixed with 4% formaldehyde (10 min), permeabilised with 0.1% Triton x-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab286823 at 1 µg/ml (shown in Red). ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488) (shown in green) was used at 2 µg/ml for structural counterstaining.
Image was acquired with a confocal microscope (Leica-Microsystems TCS SP8) and a single confocal section is shown.
This product also work with 100% methanol (5 min) fixation under the same testing conditions.
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![Alexa Fluor® 647 Anti-RBBP7 antibody [EPR23796-74] (ab286823)](https://www.abcam.co.jp/ps/products/286/ab286823/Images/ab286823-485064-Benefits-of-Recombinant-antibodies.jpg)
Alexa Fluor® 647 Anti-RBBP7 antibody [EPR23796-74] (ab286823)
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (0)
ab286823 は論文での使用が確認できていません。