AB204651

Alexa Fluor® 647 Anti-RanGAP1 抗体 [EPR3295]

Alexa Fluor® 647 Anti-RanGAP1 antibody [EPR3295]

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal RanGAP1 antibody - conjugated to Alexa Fluor® 647. Suitable for ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

別名を表示する

KIAA1835, SD, RANGAP1, Ran GTPase-activating protein 1, RanGAP1

1 Images

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-RanGAP1 antibody [EPR3295] (AB204651)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-RanGAP1 antibody [EPR3295] (AB204651)

ab204651 staining RanGAP1 in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab204651 at 1/200 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor^® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

This product also gave a positive signal under the same testing conditions in HeLa cells fixed with 100% methanol (5min).

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR3295

アイソタイプ

IgG

標識

Alexa Fluor® 647

励起波長/蛍光波長

Ex: 650nm, Em: 665nm

キャリアフリー

交差種

Human, Human, Mouse, Rat

アプリケーション

Flow Cyt (Intra), ICC/IF, IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/200", "ICCIF-species-notes": "This product gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min) and 100% methanol (5 min).", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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製品の詳細

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle|Store in the dark

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

RanGAP1 also known as Ran GTPase-activating protein 1 plays an important role in regulating nucleocytoplasmic transport. Mechanically it converts RanGTP to RanGDP in the cytoplasm which is essential for the recycling of Ran and maintenance of the Ran gradient across the nuclear envelope. The molecular mass of RanGAP1 is approximately 70 kDa. RanGAP1 is expressed in various cell types with significant presence in the cytoplasm due to its function.

Biological function summary

Many cellular processes rely on the action of RanGAP1 as it forms part of the Ran cycle. This protein is an integral component of the nuclear transport complex which regulates the bidirectional movement of RNA and proteins through the nuclear pores. Through its action RanGAP1 indirectly influences nuclear import and export playing an essential part in cell cycle regulation and mitosis.

Pathways

Several key cellular pathways are dependent on RanGAP1 activity. For instance it is a component of the mitotic spindle assembly pathway and also plays a role in the RNA processing pathway. In these pathways RanGAP1 works closely with other proteins such as RanBP1 and RCC1 facilitating important cellular events that require strict regulation of nuclear-cytoplasmic transport.

Disruptions in RanGAP1 function have been associated with certain types of cancer and neurodegenerative diseases. Aberrant regulation of RanGAP1 activity can lead to improper cell cycle progression contributing to oncogenesis. Furthermore changes in the expression of RanGAP1 and its interacting proteins like RanBP2 may relate to disorders like Huntington's disease where cellular transport mechanisms are compromised.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

GTPase activator for RAN (PubMed : 16428860, PubMed : 8146159, PubMed : 8896452). Converts cytoplasmic GTP-bound RAN to GDP-bound RAN, which is essential for RAN-mediated nuclear import and export (PubMed : 27160050, PubMed : 8896452). Mediates dissociation of cargo from nuclear export complexes containing XPO1, RAN and RANBP2 after nuclear export (PubMed : 27160050).

See full target information RANGAP1

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

The EMBO journal 37:19-38 PubMed29150432

2017

The IKK-related kinase TBK1 activates mTORC1 directly in response to growth factors and innate immune agonists.

Applications

Unspecified application

Species

Unspecified reactive species

Cagri Bodur,Dubek Kazyken,Kezhen Huang,Bilgen Ekim Ustunel,Kate A Siroky,Aaron Seth Tooley,Ian E Gonzalez,Daniel H Foley,Hugo A Acosta-Jaquez,Tammy M Barnes,Gabrielle K Steinl,Kae-Won Cho,Carey N Lumeng,Steven M Riddle,Martin G Myers,Diane C Fingar

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Alexa Fluor® 647 Anti-RanGAP1 抗体 [EPR3295]

Alexa Fluor® 647 Anti-RanGAP1 antibody [EPR3295]

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-RanGAP1 antibody [EPR3295] (AB204651)

関連する標識済み抗体及び組成の異なる製品 (4)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

標識

励起波長/蛍光波長

キャリアフリー

交差種

アプリケーション

免疫原

代替製品

Primary Antibodies

組合せ製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (1)

Abcam product promise