Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
リコンビナントRabMAb

Alexa Fluor® 647 Anti-RAB10 抗体 [MJF-R23] (ab302699)

Submit a review Submit a question
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-RAB10 antibody [MJF-R23] (AB302699)
  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-RAB10 antibody [MJF-R23] (AB302699)
  • Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-RAB10 antibody [MJF-R23] (AB302699)
  • Alexa Fluor® 647 Anti-RAB10 antibody [MJF-R23] (AB302699)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Alexa Fluor® 647 Rabbit monoclonal [MJF-R23] to RAB10
  • Suitable for: ICC/IF, Flow Cyt (Intra)
  • Reacts with: Human
  • Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 594 Carrier Free Unconjugated

製品の概要

  • 製品名

    Alexa Fluor® 647 Anti-RAB10 antibody [MJF-R23]
    RAB10 一次抗体 製品一覧

  • 製品の詳細

    Alexa Fluor® 647 Rabbit monoclonal [MJF-R23] to RAB10

  • 由来種

    Rabbit

  • 標識

    Alexa Fluor® 647. Ex: 652nm, Em: 668nm

  • アプリケーション

    適用あり: ICC/IF, Flow Cyt (Intra)more details

  • 種交差性

    交差種: Human

  • 免疫原

    Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • ICC/IF: A549 and MCF7 cells. Flow Cyt (Intra): A549 cells.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab302699の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ICC/IF
1/100.
Flow Cyt (Intra)
1/500.
特記事項
ICC/IF
1/100.
Flow Cyt (Intra)
1/500.

ターゲット情報

  • 機能

    The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion (By similarity). That Rab is mainly involved in the biosynthetic transport of proteins from the Golgi to the plasma membrane. Regulates, for instance, SLC2A4/GLUT4 glucose transporter-enriched vesicles delivery to the plasma membrane. In parallel, it regulates the transport of TLR4, a toll-like receptor to the plasma membrane and therefore may be important for innate immune response. Plays also a specific role in asymmetric protein transport to the plasma membrane within the polarized neuron and epithelial cells. In neurons, it is involved in axonogenesis through regulation of vesicular membrane trafficking toward the axonal plasma membrane while in epithelial cells, it regulates transport from the Golgi to the basolateral membrane. Moreover, may play a role in the basolateral recycling pathway and in phagosome maturation. According to PubMed:23263280, may play a role in endoplasmic reticulum dynamics and morphology controlling tubulation along microtubules and tubules fusion.

  • 配列類似性

    Belongs to the small GTPase superfamily. Rab family.

  • 細胞内局在

    Cytoplasmic vesicle membrane. Golgi apparatus membrane. Golgi apparatus, trans-Golgi network membrane. Endosome membrane. Recycling endosome membrane. Cytoplasmic vesicle, phagosome membrane. Cell projection, cilium. Endoplasmic reticulum membrane. Associates with SLC2A4/GLUT4 storage vesicles (PubMed:22908308). Localizes to the base of the cilium (PubMed:20576682). Transiently associates with phagosomes (By similarity). Localizes to the endoplasmic reticulum at domains of new tubule growth (PubMed:23263280).
  • Information by UniProt

  • 参照データベース

    • 別名

      • GTP binding protein RAB10 antibody
      • Rab10 antibody
      • RAB10 member RAS oncogene family antibody
      • RAB10_HUMAN antibody
      • Ras related GTP binding protein antibody
      • Ras related GTP binding protein RAB10 antibody
      • Ras-related protein Rab-10 antibody
      see all

    画像

    • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-RAB10 antibody [MJF-R23] (AB302699)
      Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-RAB10 antibody [MJF-R23] (AB302699)

      Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized A549 (human lung carcinoma epithelial cell) cells labeling RAB10 with ab302699 at 1/100 dilution. Confocal image showing cytoplasmic staining in A549 cells. The nuclear counter stain is DAPI (blue).

      Tubulin is detected with ab195887 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 488) at 1:200 dilution (2.5 μg/mL)(red).

    • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-RAB10 antibody [MJF-R23] (AB302699)
      Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-RAB10 antibody [MJF-R23] (AB302699)

      Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF7 (human breast adenocarcinoma epithelial cell) cells labeling RAB10 with ab302699 at 1/100 dilution. Confocal image showing cytoplasmic staining in MCF7 cells. The nuclear counter stain is DAPI (blue).

      Tubulin is detected with ab195887 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 488) at 1:200 dilution (2.5 μg/mL)(red).

    • Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-RAB10 antibody [MJF-R23] (AB302699)
      Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-RAB10 antibody [MJF-R23] (AB302699)

      Flow cytometric overlay histogram showing A549 (human lung carcinoma epithelial cell) cells labeling RAB10 with ab302699 at 1/500 dilution (red). Isotype control antibody (black) was Rabbit IgG (monoclonal) Alexa Fluor® 647 used at the same concentration and conditions as the primary antibody. Unlabeled sample (blue) was also used as a control.

    • Alexa Fluor® 647 Anti-RAB10 antibody [MJF-R23] (AB302699)
      Alexa Fluor® 647 Anti-RAB10 antibody [MJF-R23] (AB302699)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab302699 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab302699 は論文での使用が確認できていません。

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    There are currently no Customer reviews or Questions for ab302699.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com