AB225414

Alexa Fluor® 647 Anti-PROX1 抗体 [EPR19273]

Name: Alexa Fluor® 647 Anti-PROX1 抗体 [EPR19273] (ab225414) | Abcam
Brand: Abcam Limited
SKU: AB225414
Price: 88000 JPY
Availability: InStock
Rating: 3 (4 reviews)

Alexa Fluor® 647 Anti-PROX1 antibody [EPR19273]

RabMAb
Recombinant
詳細を見る

(4 Reviews)

(2 Publications)

Rabbit Recombinant Monoclonal Prox1 antibody - conjugated to Alexa Fluor® 647. Suitable for IHC-Fr, ICC/IF, IHC-P and reacts with Rat, Human, Mouse samples. Cited in 2 publications.

別名を表示する

Homeobox prospero like protein, Homeobox prospero-like protein PROX1, PROX1_HUMAN, Prospero homeobox 1, Prospero homeobox protein 1, Prospero related homeobox 1, prospero-related homeobox gene 1, zgc:111888

1 Images

Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-PROX1 antibody [EPR19273] (AB225414)

IHC-Fr

Lab

Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-PROX1 antibody [EPR19273] (AB225414)

IHC image of PROX1 staining in a section of frozen normal rat brain.

The section was fixed using 10% formaldehyde in 1XPBS for 10 minutes. No antigen retrieval step was performed prior to staining. Non-specific protein-protein interactions were then blocked in TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 1% (w/v) BSA for 1h at room temperature. The section was then incubated overnight at +4°C in TBS containing 0.025% (v/v) Triton X-100 and 1% (w/v) BSA with ab225414 at 1/100 dilution (shown in red) and counterstained using ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor^® 488), at 1/250 dilution (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount^®.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR19273

アイソタイプ

IgG

標識

Alexa Fluor® 647

励起波長/蛍光波長

Ex: 650nm, Em: 665nm

キャリアフリー

交差種

Rat, Human, Mouse

アプリケーション

IHC-P, IHC-Fr, ICC/IF

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/100", "IHCFr-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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製品の詳細

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle|Store in the dark

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

The PROX1 protein also known as Prospero homeobox 1 acts mechanically as a transcription factor and plays an important role in cell differentiation. Its molecular mass is about 83 kilodaltons. This protein is expressed in a range of tissues including the liver heart and lymphatic system. During embryonic development PROX1 is notably active where it participates in organogenesis and differentiation into specific cell types.

Biological function summary

PROX1 influences numerous processes by regulating gene expression. It forms part of several protein complexes that determine the fate of lymphatic endothelial cells balancing between lymphatic and blood vascular destinies. PROX1 directly affects the promotion and maintenance of the lymphatic endothelial cell phenotype. Its activity is fundamental to the development and maintenance of several organs and tissues impacting overall physiological function.

Pathways

The transcription factor PROX1 integrates into the VEGF signaling pathway and the Wnt signaling pathway. Within these pathways it interacts closely with other proteins such as VEGF-C and β-catenin. PROX1 modulates these pathways to orchestrate cell proliferation migration and vessel formation. Through its interactions PROX1 significantly contributes to the intricate communication between cells in developing tissues.

Research indicates that dysregulation of PROX1 is linked to cancer and lymphatic diseases. In cancer particularly colorectal and breast cancers aberrant PROX1 expression can promote tumor progression. It interacts with proteins like NF-κB which influence inflammatory responses and cell survival. Similarly in lymphedema altered PROX1 can disrupt normal lymphatic development affecting fluid balance and immune function in the body.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

See full target information Prox1

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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Science advances 11:eadv8640 PubMed40512859

2025

Interaction between NF-κB and PLAC8 impairs autophagy providing a survival advantage to prostate cells transformed by cadmium.

Applications

Unspecified application

Species

Unspecified reactive species

Vaibhav Shukla,Ashish Tyagi,Balaji Chandrasekaran,Bhawna Tyagi,Balpreet Singh,Thulasidharan Nair Devanarayanan,Venkatesh Kolluru,Murali K Ankem,Chendil Damodaran

Nature cardiovascular research 4:45-63 PubMed39747454

2025

Direct specification of lymphatic endothelium from mesenchymal progenitors.

Applications

Unspecified application

Species

Unspecified reactive species

Irina-Elena Lupu,David E Grainger,Nils Kirschnick,Sarah Weischer,Erica Zhao,Ines Martinez-Corral,Hans Schoofs,Marie Vanhollebeke,Grace Jones,Jonathan Godwin,Aden Forrow,Ines Lahmann,Paul R Riley,Thomas Zobel,Kari Alitalo,Taija Mäkinen,Friedemann Kiefer,Oliver A Stone

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Alexa Fluor® 647 Anti-PROX1 抗体 [EPR19273]

Alexa Fluor® 647 Anti-PROX1 antibody [EPR19273]

Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-PROX1 antibody [EPR19273] (AB225414)

関連する標識済み抗体及び組成の異なる製品 (2)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

標識

励起波長/蛍光波長

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (2)

代替製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

組合せ製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Abcam product promise