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AB311130

Alexa Fluor® 647 Anti-PHOX2B 抗体 [EPR14423] - C-terminal

Alexa Fluor® 647 Anti-PHOX2B antibody [EPR14423] - C-terminal

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(2 Publications)

Rabbit Recombinant Monoclonal PHOX2B antibody - conjugated to Alexa Fluor® 647. Suitable for Target Binding Affinity, Antibody Labelling, ICC/IF, IHC-P and reacts with Human, Mouse samples. Cited in 2 publications.

別名を表示する

PMX2B, PHOX2B, Paired mesoderm homeobox protein 2B, Neuroblastoma Phox, PHOX2B homeodomain protein, Paired-like homeobox 2B, NBPhox

関連する標識済み抗体及び組成の異なる製品 (7)

  • Carrier free

    Anti-PHOX2B antibody [EPR14423] - BSA and Azide free

  • Unconjugated

    Anti-PHOX2B antibody [EPR14423] - C-terminal

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-PHOX2B antibody [EPR14423] - C-terminal

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-PHOX2B antibody [EPR14423] - C-terminal

  • 603 Alexa Fluor® 568

    Alexa Fluor® 568 Anti-PHOX2B antibody [EPR14423] - C-terminal

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-PHOX2B antibody [EPR14423] - C-terminal

  • 775 Alexa Fluor® 750

    Alexa Fluor® 750 Anti-PHOX2B antibody [EPR14423] - C-terminal

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR14423

アイソタイプ

IgG

標識

Alexa Fluor® 647

励起波長/蛍光波長

Ex: 650nm, Em: 665nm

キャリアフリー

No

交差種

Human, Mouse

アプリケーション

IHC-P, ICC/IF, Antibody Labelling, Target Binding Affinity

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

特異性

The mouse recommendation is based on the WB results. We do not guarantee IHC-P for mouse.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "TargetBindingAffinity" : {"fullname" : "Target Binding Affinity", "shortname":"Target Binding Affinity"}, "AntibodyLabelling" : {"fullname" : "Antibody Labelling", "shortname":"Antibody Labelling"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "TargetBindingAffinity-species-checked": "guaranteed", "TargetBindingAffinity-species-dilution-info": "", "TargetBindingAffinity-species-notes": "", "AntibodyLabelling-species-checked": "guaranteed", "AntibodyLabelling-species-dilution-info": "", "AntibodyLabelling-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "TargetBindingAffinity-species-checked": "guaranteed", "TargetBindingAffinity-species-dilution-info": "", "TargetBindingAffinity-species-notes": "", "AntibodyLabelling-species-checked": "guaranteed", "AntibodyLabelling-species-dilution-info": "", "AntibodyLabelling-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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製品の詳細

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

How are conjugated primary antibodies validated?
This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone.

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle|Store in the dark
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

PHOX2B also known as Paired-like Homeobox 2B is a transcription factor that plays a significant role in neural development. It is encoded by the PHOX2B gene and has a molecular weight of approximately 31 kDa. This protein is highly expressed in the central and peripheral nervous systems where it influences the differentiation and function of neural crest-derived cells. PHOX2B is particularly active in the brainstem regions that control vital autonomic functions.
Biological function summary

PHOX2B influences the development of noradrenergic neurons and parasympathetic ganglia. It often functions as part of a transcription factor complex interacting with other proteins to drive the expression of genes required for the autonomic nervous system. The control PHOX2B exerts over neuronal differentiation makes it essential for maintaining the proper functioning of neurons responsible for involuntary bodily processes.

Pathways

PHOX2B is integral to the development and maintenance of the autonomic nervous and respiratory systems. It interacts closely with RET a receptor tyrosine kinase within the neurotrophic signaling pathways that regulate neuronal growth and survival. Additionally the protein plays a role in the endothelin signaling pathway further demonstrating its importance in neurodevelopment.

Alterations in PHOX2B have been linked to disorders such as congenital central hypoventilation syndrome (CCHS) and neuroblastoma. CCHS results from mutations in the PHOX2B gene affecting respiratory control. In the context of cancer PHOX2B neuroblastoma pertains to its potential role in tumor formation alongside MYCN amplification which is a common alteration in neuroblastoma. Understanding PHOX2B’s interactions provides insights into its potential as a target for therapeutic interventions.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Involved in the development of several major noradrenergic neuron populations, including the locus coeruleus. Transcription factor which could determine a neurotransmitter phenotype in vertebrates. Enhances second-messenger-mediated activation of the dopamine beta-hydrolase and c-fos promoters, and of several enhancers including cAMP-response element and serum-response element.
See full target information PHOX2B
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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:3840 PubMed40268933

2025

Neural representation of cytokines by vagal sensory neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Tomás S Huerta,Adrian C Chen,Saher Chaudhry,Aisling Tynan,Timothy Morgan,Kicheon Park,Richard Adamovich-Zeitlin,Bilal Haider,Jian Hua Li,Mitali Nagpal,Stavros Zanos,Valentin A Pavlov,Michael Brines,Theodoros P Zanos,Sangeeta S Chavan,Kevin J Tracey,Eric H Chang

Histology and histopathology 40:711-720 PubMed39363579

2024

CRISPR-mediated WNK4 point mutation aggravates tumor progression and weakens chemotherapy sensitivity in gastric cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaojun Ying,Zhen Ying,Xiaobing Gao,Yong Wang,Xinting Lv
View all publications
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Abcam product promise

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