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リコンビナントRabMAb

Alexa Fluor® 647 Anti-Pancreatic Polypeptide 抗体 [EPR23320-10] (ab300746)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Pancreatic Polypeptide antibody [EPR23320-10] (AB300746)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Pancreatic Polypeptide antibody [EPR23320-10] (AB300746)
  • Alexa Fluor® 647 Anti-Pancreatic Polypeptide antibody [EPR23320-10] (AB300746)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Alexa Fluor® 647 Rabbit monoclonal [EPR23320-10] to Pancreatic Polypeptide
  • Suitable for: IHC-P
  • Reacts with: Rat, Human
  • Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Carrier Free Unconjugated

製品の概要

  • 製品名

    Alexa Fluor® 647 Anti-Pancreatic Polypeptide antibody [EPR23320-10]
    Pancreatic Polypeptide 一次抗体 製品一覧

  • 製品の詳細

    Alexa Fluor® 647 Rabbit monoclonal [EPR23320-10] to Pancreatic Polypeptide

  • 由来種

    Rabbit

  • 標識

    Alexa Fluor® 647. Ex: 652nm, Em: 668nm

  • アプリケーション

    適用あり: IHC-Pmore details

  • 種交差性

    交差種: Rat, Human
    非交差種: Mouse

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • IHC-P: Human and rat pancreas FFPE tissue sections.

  • 特記事項

    Not suitable for mouse tissue samples

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark.

  • バッファー

    pH: 7.4
    Preservative: 0.02% Sodium azide
    Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, 68% PBS
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPR23320-10

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab300746の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-P
1/200. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
特記事項
IHC-P
1/200. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

ターゲット情報

  • 関連性

    Pancreatic hormone is synthesized in pancreatic islets of Langerhans and acts as a regulator of pancreatic and gastrointestinal functions and may be important in the regulation of food intake. Plasma levels of this hormone have been shown to be reduced in conditions associated with increased food intake and elevated in anorexia nervosa. In addition, infusion of this hormone in obese rodents has shown to decrease weight gain.

  • 細胞内局在

    Secreted

  • 参照データベース

    • 別名

      • Pancreatic polypeptide antibody
      • Pancreatic polypeptide Y antibody
      • Pancreatic prohormone antibody
      • PNP antibody
      • PP antibody
      • PPY antibody
      • prepro-PP (prepropancreatic polypeptide) antibody
      see all

    画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Pancreatic Polypeptide antibody [EPR23320-10] (AB300746)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Pancreatic Polypeptide antibody [EPR23320-10] (AB300746)

      Immunohistochemical analysis of paraffin-embedded human pancreas tissue labeling Pancreatic Polypeptide with ab300746 at 1/200 (2.5 µg/ml). The tissue section was first incubated with TBS containing 0.025% (v/v) Triton X-100, 0.3 M glycine and 1% (w/v) BSA for 1 h at room temperature to block non-specific protein-protein interactions, followed by overnight incubation at +4°C with ab300746 at 1/200 dilution (shown in red). Nuclear DNA was labeled with DAPI (shown in blue).  The section was then mounted using Fluoromount®.  Positive cytoplasmic staining was observed on human pancreatic islets.

      Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Counterstained with DAPI.

      Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0)

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Pancreatic Polypeptide antibody [EPR23320-10] (AB300746)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Pancreatic Polypeptide antibody [EPR23320-10] (AB300746)

      Immunohistochemical analysis of paraffin-embedded rat pancreas tissue labeling Pancreatic Polypeptide with ab300746 at 1/200 (2.5 µg/ml). The tissue section was first incubated with TBS containing 0.025% (v/v) Triton X-100, 0.3 M glycine and 1% (w/v) BSA for 1 h at room temperature to block non-specific protein-protein interactions, followed by overnight incubation at +4°C with ab300746 at 1/200 dilution (shown in red). Nuclear DNA was labeled with DAPI (shown in blue).  The section was then mounted using Fluoromount®.  Positive cytoplasmic staining was observed on rat pancreatic islets.

      Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

      Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0)

    • Alexa Fluor® 647 Anti-Pancreatic Polypeptide antibody [EPR23320-10] (AB300746)
      Alexa Fluor® 647 Anti-Pancreatic Polypeptide antibody [EPR23320-10] (AB300746)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab300746 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab300746 は論文での使用が確認できていません。

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