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リコンビナントRabMAb

Alexa Fluor® 647 Anti-PAI1 抗体 [EPR21850-82] (ab303581)

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-PAI1 antibody [EPR21850-82] (ab303581)
  • Alexa Fluor® 647 Anti-PAI1 antibody [EPR21850-82] (ab303581)
  • Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-PAI1 antibody [EPR21850-82] (ab303581)
  • Alexa Fluor® 647 Anti-PAI1 antibody [EPR21850-82] (ab303581)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Alexa Fluor® 647 Rabbit monoclonal [EPR21850-82] to PAI1
  • Suitable for: Flow Cyt (Intra), ICC/IF
  • Reacts with: Human
  • Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Carrier Free PE Unconjugated

製品の概要

  • 製品名

    Alexa Fluor® 647 Anti-PAI1 antibody [EPR21850-82]
    PAI1 一次抗体 製品一覧

  • 製品の詳細

    Alexa Fluor® 647 Rabbit monoclonal [EPR21850-82] to PAI1

  • 由来種

    Rabbit

  • 標識

    Alexa Fluor® 647. Ex: 652nm, Em: 668nm

  • アプリケーション

    適用あり: Flow Cyt (Intra), ICC/IFmore details

  • 種交差性

    交差種: Human

  • ポジティブ･コントロール

    • Flow Cyt (Intra): HUVEC cells. ICC/IF: HUVEC cells.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab303581の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt (Intra)
1/12500.
ICC/IF
Use a concentration of 1 µg/ml.
特記事項
Flow Cyt (Intra)
1/12500.
ICC/IF
Use a concentration of 1 µg/ml.

ターゲット情報

  • 機能

    This inhibitor acts as 'bait' for tissue plasminogen activator, urokinase, and protein C. Its rapid interaction with TPA may function as a major control point in the regulation of fibrinolysis.

  • 組織特異性

    Found in plasma and platelets and in endothelial, hepatoma and fibrosarcoma cells.

  • 関連疾患

    Defects in SERPINE1 are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1D) [MIM:613329]. It is a hematologic disorder characterized by increased bleeding after trauma, injury, or surgery. Affected females have menorrhagia. The bleeding defect is due to increased fibrinolysis of fibrin blood clots due to deficiency of plasminogen activator inhibitor-1, which inhibits tissue and urinary activators of plasminogen.
    Note=High concentrations of SERPINE1 seem to contribute to the development of venous but not arterial occlusions.

  • 配列類似性

    Belongs to the serpin family.

  • 翻訳後修飾

    Inactivated by proteolytic attack of the urokinase-type (u-PA) and the tissue-type (TPA), cleaving the 369-Arg-
    -Met-370 bond.

  • 細胞内局在

    Secreted.
  • Information by UniProt

  • 参照データベース

    • 別名

      • Clade E antibody
      • Endothelial plasminogen activator inhibitor antibody
      • Nexin antibody
      • Nexin plasminogen activator inhibitor type 1 antibody
      • PAI 1 antibody
      • PAI antibody
      • PAI-1 antibody
      • PAI1_HUMAN antibody
      • PLANH1 antibody
      • Plasminogen activator inhibitor 1 antibody
      • Plasminogen activator inhibitor type 1 antibody
      • Serine (or cysteine) proteinase inhibitor antibody
      • Serine (or cysteine) proteinase inhibitor clade E (nexin plasminogen activator inhibitor type 1) member 1 antibody
      • Serine proteinase inhibitor clade E member 1 antibody
      • serpin antibody
      • Serpin E1 antibody
      • Serpin peptidase inhibitor clade E (nexin plasminogen activator inhibitor type 1) member 1 antibody
      • Serpin peptidase inhibitor clade E antibody
      • Serpine 1 antibody
      • SERPINE1 antibody
      see all

    画像

    • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-PAI1 antibody [EPR21850-82] (ab303581)
      Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-PAI1 antibody [EPR21850-82] (ab303581)

      AB303581 staining PAI-1 in HUVEC (Human umbilical vein epithelial cell line) cells (positive control) and HEK293 cells (negative control). The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton x-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3 M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with AB303581 at 1 µg/ml (shown in red) and ab195883 (Alexa Fluor®488 Anti-Tubulin antibody [YOL1/34]) at 5 µg/ml (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). Image was acquired with a confocal microscope (Leica-Microsystems TCS SP8) and a single confocal section is shown.

    • Alexa Fluor® 647 Anti-PAI1 antibody [EPR21850-82] (ab303581)
      Alexa Fluor® 647 Anti-PAI1 antibody [EPR21850-82] (ab303581)
    • Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-PAI1 antibody [EPR21850-82] (ab303581)
      Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-PAI1 antibody [EPR21850-82] (ab303581)

      Flow cytometry overlay histogram showing left HUVEC positive cells and right negative HEK293 cells stained with AB303581 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interaction followed by the antibody (AB303581) (1x 106 in 100μl at 0.04μg/ml (1/12500)) for 30 min at 22°C.

      Isotype control antibody (black line) was used at the same concentration and conditions as the primary antibody. Unlabeled sample (blue line) was also used as a control.

      Acquisition of >5000 events were collected using a 40 mW Red laser (638 nm) and 660/10 bandpass filter.

      This antibody gave a positive signal in HUVEC cells fixed with 80 % methanol (5 min) / permeabilized with 0.1% PBS-Triton X-100 for 15 min used under the same conditions.

       

    • Alexa Fluor® 647 Anti-PAI1 antibody [EPR21850-82] (ab303581)
      Alexa Fluor® 647 Anti-PAI1 antibody [EPR21850-82] (ab303581)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab303581 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab303581 は論文での使用が確認できていません。

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