AB237265

Alexa Fluor® 647 Anti-p21 抗体 [EPR18021]

Alexa Fluor® 647 Anti-p21 antibody [EPR18021]

RabMAb
Recombinant
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(5 Publications)

Anti-p21 antibody [EPR18021] - Alexa Fluor® 647 conjugated (ab237265) is a rabbit recombinant monoclonal antibody detecting p21 in Flow Cytometry (Intra), Flow Cytometry, ICC/IF. Suitable for Mouse.

- Biophysical QC for unrivalled batch-batch consistency

別名を表示する

Cip1, Waf1, Cdkn1a, Cyclin-dependent kinase inhibitor 1, CDK-interacting protein 1, Melanoma differentiation-associated protein, p21

2 Images

Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-p21 antibody [EPR18021] (AB237265)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-p21 antibody [EPR18021] (AB237265)

Overlay histogram showing NIH/3T3 cells stained with ab237265 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS/10% normal Goat serum to block non-specific protein-protein interactions followed by the antibody (ab237265) (1x10⁶ in 100 μl at 0.2 μg/ml (1/2500 dilution)) for 30 min at 22°C.

Isotype control antibody (black line) was Rabbit IgG (monoclonal) Alexa Fluor^® 647 (ab199093) used at the same concentration and conditions as the primary antibody. Unlabeled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 40 mW Red laser (640nm) and 670/14 bandpass filter.

This antibody gave a positive signal in NIH/3T3 cells fixed with 4% formaldehyde (10 min)/permeabilized with 0.1% PBS-Triton X-100 for 15 min used under the same conditions.

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-p21 antibody [EPR18021] (AB237265)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-p21 antibody [EPR18021] (AB237265)

ab237265 staining p21 in NIH/3T3 cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab237265 at 1/1000 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor^® 488), at 1/250 dilution (shown in green). Nuclear DNA was labeled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR18021

アイソタイプ

IgG

標識

Alexa Fluor® 647

励起波長/蛍光波長

Ex: 650nm, Em: 665nm

キャリアフリー

交差種

Mouse

アプリケーション

IHC-P, ICC/IF, Flow Cyt (Intra)

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

特異性

Expression levels of the target protein vary between different tissue/cell lines and in some cases induction may be required before a signal is observed.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/1000", "ICCIF-species-notes": "This product gave a positive signal in NIH3T3 fixed with 4% formaldehyde (10 min).", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/2500", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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製品の詳細

What is this antibody validated in?
Alexa Fluor® 647 Anti-p21 antibody [EPR18021] (ab237265) is a rabbit recombinant monoclonal antibody and is validated for use in Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunocytochemistry/immunofluorescence (ICC/IF) in Mouse samples.

Related products
Antibody clone EPR18021 is also available pre-conjugated to a variety of labels for your convenience – Anti-p21 Alexa Fluor® 647 [EPR18021] (ab237265).

Other related products
We have a range of other formats of antibody clone [EPR18021] also available for your convenience: ab188224, Carrier free - ab232512, Alexa Fluor® 488 - ab237264, Alexa Fluor® 647 - ab237265, Carrier free - ab242468, APC - ab313956, PE - ab314287

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle|Stable for 12 months at -20°C|Store in the dark

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

The p21 protein also known as CDKN1A is an important regulator of cell cycle progression. It acts as a cyclin-dependent kinase inhibitor where it binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes. The molecular weight of p21 is approximately 21 kDa. Cellular expression of p21 is diverse; it occurs in various tissues reflecting its broad role in maintaining cell cycle control. This protein's expression level is often assessed using techniques such as p21 western blotting p21 ELISA and p21 IHC staining.

Biological function summary

P21 plays a critical role in cell cycle regulation and DNA damage response. It associates with the p53 tumor suppressor protein forming an essential part of the p53 signaling pathway under stress conditions. p21 arrests cells in G1 phase allowing DNA repair or leading to cellular senescence. Through these mechanisms p21 integrates signals from stress and damage emphasizing its importance as a mediator in cellular checkpoint control.

Pathways

P21 is closely involved in the p53 and the Transforming Growth Factor-beta (TGF-Β) signaling pathways. This integration helps in transmitting growth-inhibitory signals. p21 interacts with proteins such as p53 and cyclins ensuring precise regulation of the cell cycle and growth arrest when necessary. Triggered by p53 activation p21 contributes to preventing undisturbed cell proliferation acting as a safeguard against tumor formation.

P21's deregulation associates with cancer and age-related diseases. Its impairment is a common feature in many cancers often linked with defects in the p53 pathway. Reduced p21 expression can contribute to unchecked cell division and tumor progression. Moreover in age-related diseases p21's role in promoting cellular senescence connects it with degenerative conditions. The intricate relationship between p21 and proteins such as p53 and cyclins highlights its potential as a therapeutic target for these disorders.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

May be involved in p53/TP53 mediated inhibition of cellular proliferation in response to DNA damage. Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin-dependent kinase substrates and blocking cell cycle progression. Functions in the nuclear localization and assembly of cyclin D-CDK4 complex and promotes its kinase activity towards RB1. At higher stoichiometric ratios, inhibits the kinase activity of the cyclin D-CDK4 complex (PubMed : 25329316). Inhibits DNA synthesis by DNA polymerase delta by competing with POLD3 for PCNA binding (By similarity). Plays an important role in controlling cell cycle progression and DNA damage-induced G2 arrest (By similarity).. Plays an important role in controlling cell cycle progression and DNA damage-induced G2 arrest (PubMed : 37178686). Involved in p53/TP53 mediated inhibition of cellular proliferation in response to DNA damage. Also involved in p53-independent DNA damage-induced G2 arrest mediated by CREB3L1 in astrocytes and osteoblasts (PubMed : 37178686). Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin-dependent kinase substrates and blocking cell cycle progression. Functions in the nuclear localization and assembly of cyclin D-CDK4 complex and promotes its kinase activity towards RB1. At higher stoichiometric ratios, inhibits the kinase activity of the cyclin D-CDK4 complex (PubMed : 25329316). Inhibits DNA synthesis by DNA polymerase delta by competing with POLD3 for PCNA binding (By similarity). Negatively regulates the CDK4- and CDK6-driven phosphorylation of RB1 in keratinocytes, thereby resulting in the release of E2F1 and subsequent transcription of E2F1-driven G1/S phase promoting genes (PubMed : 36689330).

See full target information Cdkn1a

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文献 (5)

Recent publications for all applications. Explore the full list and refine your search

Experimental & molecular medicine 57:1861-1877 PubMed40855115

2025

Mitochondrial-encoded peptide MOTS-c prevents pancreatic islet cell senescence to delay diabetes.

Applications

Unspecified application

Species

Unspecified reactive species

Byung Soo Kong,Hyunsuk Lee,Sehi L'Yi,Serin Hong,Young Min Cho

Science advances 11:eadv6642 PubMed40668916

2025

Heterochronic parabiosis uncovers AdipoR1 as a critical player in retinal rejuvenation.

Applications

Unspecified application

Species

Unspecified reactive species

Yidan Liu,Xiuxing Liu,Jianjie Lv,Qi Zhang,Zhenlan Yang,Xuhao Chen,Chenyang Gu,Chun Zhang,Yehong Zhuo,Wenru Su

Diabetes & metabolism journal 49:1229-1241 PubMed40403762

2025

Anti-Senescence Effect of Inhibiting Sodium-Glucose Cotransporter 2 and α-Glucosidase in a Type 2 Diabetes Mellitus Animal Model.

Applications

Unspecified application

Species

Unspecified reactive species

Serin Hong,Byung Soo Kong,Hyunsuk Lee,Young Min Cho

Nature aging 5:799-815 PubMed40307497

2025

KLRG1 identifies regulatory T cells with mitochondrial alterations that accumulate with aging.

Applications

Unspecified application

Species

Unspecified reactive species

Gonzalo Soto-Heredero,Enrique Gabandé-Rodríguez,Elisa Carrasco,José Ignacio Escrig-Larena,Manuel M Gómez de Las Heras,Sandra Delgado-Pulido,Isaac Francos-Quijorna,Eva M Blanco,Álvaro Fernández-Almeida,David Abia,María Josefa Rodríguez,Cristina M Fernández-Díaz,María Beatriz Álvarez-Flores,Ana Ramírez de Molina,Sascha Jung,Antonio Del Sol,Virginia Zorita,Fátima Sánchez-Cabo,Carlos Torroja,María Mittelbrunn

Nature genetics 53:672-682 PubMed33833453

2021

Silencing of LINE-1 retrotransposons is a selective dependency of myeloid leukemia.

Applications

Unspecified application

Species

Unspecified reactive species

Zhimin Gu,Yuxuan Liu,Yuannyu Zhang,Hui Cao,Junhua Lyu,Xun Wang,Annika Wylie,Simon J Newkirk,Amanda E Jones,Michael Lee,Giovanni A Botten,Mi Deng,Kathryn E Dickerson,Cheng Cheng Zhang,Wenfeng An,John M Abrams,Jian Xu

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Alexa Fluor® 647 Anti-p21 抗体 [EPR18021]

Alexa Fluor® 647 Anti-p21 antibody [EPR18021]

Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-p21 antibody [EPR18021] (AB237265)

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-p21 antibody [EPR18021] (AB237265)

関連する標識済み抗体及び組成の異なる製品 (6)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

標識

励起波長/蛍光波長

キャリアフリー

交差種

アプリケーション

免疫原

特異性

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (5)

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Abcam product promise