AB252131

Alexa Fluor® 647 Anti-Myeloperoxidase 抗体 [EPR17996]

Name: Alexa Fluor® 647 Anti-Myeloperoxidase 抗体 [EPR17996] (ab252131) | Abcam
Brand: Abcam Limited
SKU: AB252131
Price: 82000 JPY
Availability: InStock
Rating: 4 (1 reviews)

Alexa Fluor® 647 Anti-Myeloperoxidase antibody [EPR17996]

RabMAb
Recombinant
詳細を見る

(1 Review)

(7 Publications)

Rabbit Recombinant Monoclonal Myeloperoxidase antibody - conjugated to Alexa Fluor® 647. Suitable for IHC-P and reacts with Mouse, Human samples. Cited in 7 publications.

別名を表示する

Myeloperoxidase, MPO

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Myeloperoxidase antibody [EPR17996] (AB252131)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Myeloperoxidase antibody [EPR17996] (AB252131)

IHC image of Myeloperoxidase staining in a section of formalin-fixed paraffin-embedded normal human spleen*.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) in a Biocare Medical NxGen pressure cooker using retrieval settings of 110°C for 20 minutes. Non-specific protein-protein interactions were then blocked in TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 1% (w/v) BSA for 1h at room temperature. The section was then incubated overnight at +4°C in TBS containing 0.025% (v/v) Triton X-100 and 1% (w/v) BSA with ab252131 at 1/100 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount^®.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Myeloperoxidase antibody [EPR17996] (AB252131)

IHC image of Myeloperoxidase staining in a section of formalin-fixed paraffin-embedded normal mouse spleen*.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

*Tissue obtained from .

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR17996

アイソタイプ

IgG

標識

Alexa Fluor® 647

励起波長/蛍光波長

Ex: 650nm, Em: 665nm

キャリアフリー

交差種

Mouse, Human

アプリケーション

IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p>" } } }

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製品の詳細

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle|Store in the dark

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Myeloperoxidase also called MPO is an enzyme that plays a critical role in the body's immune response. This protein has a mass of approximately 150 kDa and exists as a dimer composed of heavy and light polypeptide chains. Myeloperoxidase is prominently expressed in neutrophils and monocytes which are types of white blood cells important for combating infections. The enzyme catalyzes the production of hypochlorous acid and other reactive substances by utilizing hydrogen peroxide and chloride ions. These reactive substances help in neutralizing pathogens during the immune response.

Biological function summary

The generation of reactive oxygen species by myeloperoxidase is essential for microbicidal activity. Myeloperoxidase functions as part of the antimicrobial system in the phagosome which is the intracellular compartment where pathogens are degraded. This enzyme works in conjunction with other components of the immune system such as NADPH oxidase. By generating hypochlorous acid MPO contributes to the oxidative burst a rapid release of reactive oxygen species during the response to pathogens.

Pathways

Myeloperoxidase integrates into the immune defense and inflammatory pathways. In particular it is associated with the neutrophil degranulation pathway where it releases its enzymatic contents to fight off microbes. MPO also interacts with proteins involved in oxidative stress processes such as superoxide dismutase which moderates levels of reactive oxygen species in cells. These interactions ensure balance in the immune response preventing excessive tissue damage during inflammation.

Dysregulated MPO activity can contribute to the development of diseases. For instance myeloperoxidase is linked with atherosclerosis a cardiovascular condition where inflammation and oxidative stress lead to plaque formation in the arteries. It also associates with vasculitis an autoimmune disorder causing inflammation of blood vessels. Both disorders can relate to the inflammatory pathways that involve MPO and proteins like C-reactive protein which serves as a marker of inflammation. Understanding MPO's role in these conditions is important for effective therapeutic interventions.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity (PubMed : 9922160). Mediates the proteolytic cleavage of alpha-1-microglobulin to form t-alpha-1-microglobulin, which potently inhibits oxidation of low-density lipoprotein particles and limits vascular damage (PubMed : 25698971).

See full target information MPO

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文献 (7)

Recent publications for all applications. Explore the full list and refine your search

British journal of pharmacology 182:5057-5070 PubMed40258390

2025

FGF1 ameliorates DSS-induced ulcerative colitis by reducing neutrophil recruitment through the MAPK pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Shuang Feng,Yanyan Jin,Xinrui Ni,Haoxin Zheng,Linling Wu,Ying Xia,Changzhi Zhou,Tong Liang,Yunfei Zhu,Juyi Xu,Qijin Wu,Yong Yang,Longwei Zhao,Shentian Zhuang,Xianjing Li

Frontiers in oncology 15:1543657 PubMed40196735

2025

RAS70 peptide targets multiforme glioblastoma by binding to the plasma membrane heat shock protein HSP70.

Applications

Unspecified application

Species

Unspecified reactive species

Maxim Shevtsov,Natalia Yudintceva,Danila Bobkov,Ruslana Likhomanova,Anastasiya Nechaeva,Elena Mikhailova,Elena Oganesyan,Viacheslav Fedorov,Andrey Kurkin,Anastasiya Lukacheva,Georgii Fofanov,Aleksander Kim,Evegeniy Fedorov,Daria Sitovskaya,Alexey Ulitin,Natalia Mikhailova,Ilya Anufriev,Maria Istomina,Ekaterina Murashko,Elizaveta Kessenikh,Nikolay Aksenov,Yulia Vakhitova,Konstantin Samochernykh,Emil Pitkin,Evgeny Shlyakhto,Stephanie E Combs

BMC immunology 26:3 PubMed39806304

2025

IL-6 and PD-1 antibody blockade combination therapy regulate inflammation and T lymphocyte apoptosis in murine model of sepsis.

Applications

Unspecified application

Species

Unspecified reactive species

Song I Lee,Na Young Kim,Chaeuk Chung,Dongil Park,Da Hyun Kang,Duk Ki Kim,Min-Kyung Yeo,Pureum Sun,Jeong Eun Lee

Journal of immunology research 2024:5556042 PubMed39534554

2024

Manual Therapy Exerts Local Anti-Inflammatory Effects Through Neutrophil Clearance.

Applications

Unspecified application

Species

Unspecified reactive species

Hongwen Liu,Shiguo Yuan,Kai Zheng,Gaofeng Liu,Junhua Li,Baofei Ye,Yangkun Wang,Li Yin,Yikai Li

PloS one 18:e0289183 PubMed37963142

2023

An intravenous pancreatic cancer therapeutic: Characterization of CRISPR/Cas9n-modified Clostridium novyi-Non Toxic.

Applications

Unspecified application

Species

Unspecified reactive species

Kaitlin M Dailey,James M Small,Jessica E Pullan,Seth Winfree,Krysten E Vance,Megan Orr,Sanku Mallik,Kenneth W Bayles,Michael A Hollingsworth,Amanda E Brooks

Cellular and molecular gastroenterology and hepatology 14:905-924 PubMed35835392

2022

A Novel Three-Dimensional Imaging System Based on Polysaccharide Staining for Accurate Histopathological Diagnosis of Inflammatory Bowel Diseases.

Applications

Unspecified application

Species

Unspecified reactive species

Satoshi Nojima,Shoichi Ishida,Kei Terayama,Katsuhiko Matsumoto,Takahiro Matsui,Shinichiro Tahara,Kenji Ohshima,Hiroki Kiyokawa,Kansuke Kido,Koto Ukon,Shota Y Yoshida,Tomoki T Mitani,Yuichiro Doki,Tsunekazu Mizushima,Yasushi Okuno,Etsuo A Susaki,Hiroki R Ueda,Eiichi Morii

The American journal of pathology 192:112-120 PubMed34599881

2021

Coronavirus Disease 2019 (COVID-19) Coronary Vascular Thrombosis: Correlation with Neutrophil but Not Endothelial Activation.

Applications

Unspecified application

Species

Unspecified reactive species

Justin E Johnson,Declan McGuone,Mina L Xu,Dan Jane-Wit,Richard N Mitchell,Peter Libby,Jordan S Pober

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Alexa Fluor® 647 Anti-Myeloperoxidase 抗体 [EPR17996]

Alexa Fluor® 647 Anti-Myeloperoxidase antibody [EPR17996]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Myeloperoxidase antibody [EPR17996] (AB252131)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Myeloperoxidase antibody [EPR17996] (AB252131)

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Key facts

宿主種

クローン性

クローン番号

アイソタイプ

標識

励起波長/蛍光波長

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (7)

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Abcam product promise