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AB204478

Alexa Fluor® 647 Anti-MKLP1 抗体 [EPR10879]

Alexa Fluor® 647 Anti-MKLP1 antibody [EPR10879]

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(2 Publications)

Rabbit Recombinant Monoclonal MKLP1 antibody - conjugated to Alexa Fluor® 647. Suitable for ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 2 publications.

別名を表示する

KNSL5, MKLP1, KIF23, Kinesin-like protein KIF23, Kinesin-like protein 5, Mitotic kinesin-like protein 1

1 Images
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-MKLP1 antibody [EPR10879] (AB204478)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-MKLP1 antibody [EPR10879] (AB204478)

ab204478 staining MKLP1 in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab204478 at a 1/100 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at a 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

This product also gave a positive signal under the same testing conditions in HeLa cells fixed with 100% methanol (5 min).

関連する標識済み抗体及び組成の異なる製品 (2)

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR10879

アイソタイプ

IgG

標識

Alexa Fluor® 647

励起波長/蛍光波長

Ex: 650nm, Em: 665nm

キャリアフリー

No

交差種

Human, Human, Mouse, Rat

アプリケーション

ICC/IF

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p>This product gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min) and 100% methanol (5 min).</p>" }, "Mouse": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Rat": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
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製品の詳細

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Stable for 12 months at -20°C|Store in the dark
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

MKLP1 also known as mitotic kinesin-like protein 1 is a motor protein with a molecular weight of around 95 kDa. This protein plays an important role in cell division by facilitating the movement of chromosomes and the formation of the spindle apparatus during mitosis. MKLP1 is widely expressed in proliferating cells and tissues especially in those undergoing rapid cell cycles such as embryonic tissues and tumors.
Biological function summary

MKLP1 functions as a component of the centralspindlin complex where it interacts with other proteins to regulate cytoskeletal dynamics. It is critical in the formation and resolution of the central spindle during mitosis ensuring proper separation of cells. By coordinating microtubule bundling and motor activities MKLP1 assists in the maintenance of cell polarity and cell shape changes necessary for mitosis completion.

Pathways

MKLP1 is integral to the mitotic and cell cycle pathways. In particular it is important in the mechanics of the spindle assembly checkpoint which ensures chromosomes are correctly segregated before cell division proceeds. MKLP1 interacts with proteins like Aurora B kinase and ECT2 which regulate the dynamics of cytoskeletal reorganization during these critical phases.

MKLP1 has associations with cancers and neurodevelopmental disorders. In tumors overexpression or mutation of MKLP1 often leads to aneuploidy contributing to tumor progression and metastasis. In the context of neurodevelopmental disorders disruptions in MKLP1 function can relate to conditions such as microcephaly due to its role in cell proliferation. Altered interactions between MKLP1 and proteins like BRCA2 in cancer highlight its relevance in both cell division and stability of the genome.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Component of the centralspindlin complex that serves as a microtubule-dependent and Rho-mediated signaling required for the myosin contractile ring formation during the cell cycle cytokinesis. Essential for cytokinesis in Rho-mediated signaling. Required for the localization of ECT2 to the central spindle. Plus-end-directed motor enzyme that moves antiparallel microtubules in vitro.
See full target information KIF23
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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of molecular cell biology 12:654-665 PubMed31174204

2019

Mitotic motor CENP-E cooperates with PRC1 in temporal control of central spindle assembly.

Applications

Unspecified application

Species

Unspecified reactive species

Xu Liu,Leilei Xu,Junying Li,Phil Y Yao,Wanjuan Wang,Hazrat Ismail,Haowei Wang,Bryce Liao,Zhihong Yang,Tarsha Ward,Ke Ruan,Jianchun Zhang,Quan Wu,Ping He,Xia Ding,Dongmei Wang,Chuanhai Fu,Zhen Dou,Feng Yan,Wenwen Wang,Xing Liu,Xuebiao Yao

Biomedicine & pharmacotherapy = Biomedecine & phar 108:271-279 PubMed30223098

2018

microRNA-300/NAMPT regulates inflammatory responses through activation of AMPK/mTOR signaling pathway in neonatal sepsis.

Applications

Unspecified application

Species

Unspecified reactive species

Yexuzi Li,Junzhong Ke,Chen Peng,Fugen Wu,Yukang Song
View all publications
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代替製品

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AB168964

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組合せ製品

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Primary Antibodies

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Alexa Fluor® 647 Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker

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Alexa Fluor® 555 Anti-beta Tubulin antibody [EPR16774] - Microtubule Marker

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