Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
リコンビナントRabMAb

Alexa Fluor® 647 Anti-LINE-1 ORF1p 抗体 [EPR21844-108] (ab303593)

Submit a review Submit a question
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-LINE-1 ORF1p antibody [EPR21844-108] (ab303593)
  • Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-LINE-1 ORF1p antibody [EPR21844-108] (ab303593)
  • Alexa Fluor® 647 Anti-LINE-1 ORF1p antibody [EPR21844-108] (ab303593)
  • Alexa Fluor® 647 Anti-LINE-1 ORF1p antibody [EPR21844-108] (AB303593)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Alexa Fluor® 647 Rabbit monoclonal [EPR21844-108] to LINE-1 ORF1p
  • Suitable for: Flow Cyt (Intra), ICC/IF
  • Reacts with: Mouse
  • Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm

Conjugates logo Related conjugates and formulations

Carrier Free Unconjugated

製品の概要

  • 製品名

    Alexa Fluor® 647 Anti-LINE-1 ORF1p antibody [EPR21844-108]
    LINE-1 ORF1p 一次抗体 製品一覧

  • 製品の詳細

    Alexa Fluor® 647 Rabbit monoclonal [EPR21844-108] to LINE-1 ORF1p

  • 由来種

    Rabbit

  • 標識

    Alexa Fluor® 647. Ex: 652nm, Em: 668nm

  • アプリケーション

    適用あり: Flow Cyt (Intra), ICC/IFmore details

  • 種交差性

    交差種: Mouse

  • 免疫原

    Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • Flow Cyt (Intra): F9 cells. ICC/IF: F9 cells.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark.

  • バッファー

    pH: 7.4
    Preservative: 0.02% Sodium azide
    Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPR21844-108

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab303593の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt (Intra)
1/2500.
ICC/IF
Use a concentration of 0.2 µg/ml.
特記事項
Flow Cyt (Intra)
1/2500.
ICC/IF
Use a concentration of 0.2 µg/ml.

ターゲット情報

  • 関連性

    Domain: The coiled coil domain mediates homotrimerization. The RRM and the CTD domain are both required for proper RNA-binding activity. Function: Nucleic acid-binding protein which is essential for retrotransposition of LINE-1 elements in the genome. Functions as a nucleic acid chaperone binding its own transcript and therefore preferentially mobilizing the transcript from which they are encoded. Long interspersed element-1/LINE-1/L1 retrotransposons are present in more than 500'000 full (6 kb) or truncated copies in the human genome. Most of them are inactive but 80 to 100 of those elements could be transcribed, translated and active in any individual. An active LINE-1 encodes for 2 proteins translated from a single RNA containing 2 non-overlapping ORFs, ORF1 and ORF2. ORF1p is described in this entry as a representative of all ORF1p potentially expressed by active elements. ORF2p is described in the related entry AC O00370. PTM: Polyubiquitinated, probably by UBR2, which induces its degradation. Similarity: Belongs to the transposase 22 family.

  • 参照データベース

    • 別名

      • L1ORF1p antibody
      • L1RE1 antibody
      • LINE retrotransposable element 1 antibody
      • LINE-1 retrotransposable element ORF1 protein antibody
      • LINE1 retrotransposable element 1 antibody
      • LRE1 antibody
      see all

    画像

    • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-LINE-1 ORF1p antibody [EPR21844-108] (ab303593)
      Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-LINE-1 ORF1p antibody [EPR21844-108] (ab303593)

      AB303593 staining LINE-1 ORF1p in F9 (Mouse embryonic testicular cancer cell line) cells (positive control) and NIH/3T3 cells (negative control). The cells were fixed with 100% methanol (10 min), permeabilized with 0.1% Triton x-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3 M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with AB303593 at 0.2 µg/ml (shown in red) and ab195883 (Alexa Fluor®488 Anti-Tubulin antibody [YOL1/34]) at 5 µg/ml (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). Image was acquired with a confocal microscope (Leica-Microsystems TCS SP8) and a single confocal section is shown.

    • Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-LINE-1 ORF1p antibody [EPR21844-108] (ab303593)
      Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-LINE-1 ORF1p antibody [EPR21844-108] (ab303593)

      Flow cytometry overlay histogram showing left F9 positive cells and right negative NIH3T3 cells stained with AB303593 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interaction followed by the antibody (AB303593) (1x 106 in 100μl at 0.2μg/ml (1/2500)) for 30 min at 22°C.

      Isotype control antibody (black line) was used at the same concentration and conditions as the primary antibody. Unlabeled sample (blue line) was also used as a control.

      Acquisition of >5000 events were collected using a 40 mW Red laser (638 nm) and 660/10 bandpass filter.

    • Alexa Fluor® 647 Anti-LINE-1 ORF1p antibody [EPR21844-108] (ab303593)
      Alexa Fluor® 647 Anti-LINE-1 ORF1p antibody [EPR21844-108] (ab303593)
    • Alexa Fluor® 647 Anti-LINE-1 ORF1p antibody [EPR21844-108] (AB303593)
      Alexa Fluor® 647 Anti-LINE-1 ORF1p antibody [EPR21844-108] (AB303593)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab303593 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab303593 は論文での使用が確認できていません。

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    There are currently no Customer reviews or Questions for ab303593.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com