AB237307

Alexa Fluor® 647 Anti-LAMP1 抗体 [EPR21026]

Alexa Fluor® 647 Anti-LAMP1 antibody [EPR21026] - Lysosome Marker

RabMAb
Recombinant
詳細を見る

Be the first to review this product! Submit a review

(6 Publications)

Rabbit Recombinant Monoclonal LAMP1 antibody - conjugated to Alexa Fluor® 647. Suitable for ICC/IF and reacts with Mouse samples. Cited in 6 publications.

別名を表示する

CD107a, Lamp-1, Lamp1, Lysosome-associated membrane glycoprotein 1, LAMP-1, Lysosome-associated membrane protein 1, 120 kDa lysosomal membrane glycoprotein, CD107 antigen-like family member A, LGP-120, Lysosomal membrane glycoprotein A, P2B, LGP-A

1 Images

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-LAMP1 antibody [EPR21026] - Lysosome Marker (AB237307)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-LAMP1 antibody [EPR21026] - Lysosome Marker (AB237307)

ab237307 staining LAMP1 in NIH/3T3 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab237307 at 1/100 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor^® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR21026

アイソタイプ

IgG

標識

Alexa Fluor® 647

励起波長/蛍光波長

Ex: 650nm, Em: 665nm

キャリアフリー

交差種

Mouse

アプリケーション

ICC/IF

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p>This product gave a positive signal in NIH/3T3 fixed with 100% methanol (5 min).</p>" } } }

style

left-column

製品の詳細

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

style

left-column

出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle|Stable for 12 months at -20°C|Store in the dark

style

left-column

補足情報

This supplementary information is collated from multiple sources and compiled automatically.

LAMP1 also known as lysosome-associated membrane glycoprotein 1 is an important player within cellular mechanics. It exhibits a molecular weight of approximately 120 kDa. This protein exists abundantly in lysosomal membranes and sometimes in endosomes. LAMP1 operates near most cell types and has a significant presence in immune neuronal and epithelial cells. By maintaining the lysosomal membrane's integrity LAMP1 contributes to cellular homeostasis.

Biological function summary

LAMP1 functions to protect lysosomal membranes from the harsh environment inside the lysosome. It forms part of a protective glycocalyx composed of highly glycosylated proteins supporting lysosomal stability. This protein interacts closely with other lysosome markers and assists in the fusion of vesicles with lysosomes making it fundamental to lysosomal processes. As a lysosome marker LAMP1 helps identify lysosomal compartments during studies involving microscopy and LAMP1 staining.

Pathways

The protein LAMP1 participates in the autophagy and endocytic pathways. It acts collaboratively within the lysosomal degradation route involving proteins like LAMP2 which also supports lysosomal function. LAMP1 influences these pathways by mediating the fusion of autophagosomes or endosomes with lysosomes thereby ensuring efficient breakdown of cellular debris and macromolecules.

LAMP1 associates with lysosomal storage disorders and neurodegenerative diseases. The abnormal function or expression of LAMP1 has been linked to conditions such as Niemann-Pick disease and Alzheimer's disease. In these contexts its interaction with proteins like APP (amyloid precursor protein) illustrates its involvement in pathogenic pathways that lead to cellular dysfunction and disease progression. Understanding LAMP1's role may enhance diagnostic and therapeutic approaches pertaining to these disorders.

style

left-column

style

left-column

製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

ターゲットの情報

Lysosomal membrane glycoprotein which plays an important role in lysosome biogenesis, lysosomal pH regulation, autophagy and cholesterol homeostasis (PubMed : 15121881). Acts as an important regulator of lysosomal lumen pH regulation by acting as a direct inhibitor of the proton channel TMEM175, facilitating lysosomal acidification for optimal hydrolase activity (By similarity). Also plays an important role in NK-cells cytotoxicity (PubMed : 23847195). Mechanistically, participates in cytotoxic granule movement to the cell surface and perforin trafficking to the lytic granule (By similarity). In addition, protects NK-cells from degranulation-associated damage induced by their own cytotoxic granule content (PubMed : 23847195). Presents carbohydrate ligands to selectins (By similarity). Also implicated in tumor cell metastasis (PubMed : 2676155).

See full target information Lamp1

style

left-column

文献 (6)

Recent publications for all applications. Explore the full list and refine your search

The Journal of biological chemistry 301:110504 PubMed40701245

2025

PERK and IRE1α promote exosome secretion via blocking lysosomal degradation of multiple vesicular body.

Applications

Unspecified application

Species

Unspecified reactive species

Shixin Zhou,Zihan Zhou,Si Chen,Ming Wang,Likun Wang

Nature communications 16:1175 PubMed39885146

2025

Oligodendrocyte precursor cells facilitate neuronal lysosome release.

Applications

Unspecified application

Species

Unspecified reactive species

Li-Pao Fang,Ching-Hsin Lin,Yasser Medlej,Renping Zhao,Hsin-Fang Chang,Qilin Guo,Zhonghao Wu,Yixun Su,Na Zhao,Davide Gobbo,Amanda Wyatt,Vanessa Wahl,Frederic Fiore,Szu-Min Tu,Ulrich Boehm,Wenhui Huang,Shan Bian,Amit Agarwal,Marcel A Lauterbach,Chenju Yi,Jianqin Niu,Anja Scheller,Frank Kirchhoff,Xianshu Bai

Journal of leukocyte biology 116:632-643 PubMed38484156

2024

B-1a cells scavenge NETs to attenuate sepsis.

Applications

Unspecified application

Species

Unspecified reactive species

Kensuke Murata,Atsushi Murao,Chuyi Tan,Ping Wang,Monowar Aziz

Microbiology spectrum 11:e0225323 PubMed37796020

2023

Lactate promotes intracellular replication and systemic infection via driving macrophage M2 polarization.

Applications

Unspecified application

Species

Unspecified reactive species

Xinyue Wang,Bin Yang,Shuangshuang Ma,Xiaolin Yan,Shuai Ma,Hongmin Sun,Yuyang Sun,Lingyan Jiang

Nature immunology 24:991-1006 PubMed37095377

2023

Dynamic mitochondrial transcription and translation in B cells control germinal center entry and lymphomagenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Yavuz F Yazicioglu,Eros Marin,Ciaran Sandhu,Silvia Galiani,Iwan G A Raza,Mohammad Ali,Barbara Kronsteiner,Ewoud B Compeer,Moustafa Attar,Susanna J Dunachie,Michael L Dustin,Alexander J Clarke

Scientific reports 13:6393 PubMed37076504

2023

Dentin defects caused by a Dspp frameshift mutation are associated with the activation of autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Tian Liang,Charles E Smith,Yuanyuan Hu,Hong Zhang,Chuhua Zhang,Qian Xu,Yongbo Lu,Ling Qi,Jan C-C Hu,James P Simmer

View all publications

style

left-column

style

left-column

style

right-column

Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Alexa Fluor® 647 Anti-LAMP1 抗体 [EPR21026]

Alexa Fluor® 647 Anti-LAMP1 antibody [EPR21026] - Lysosome Marker

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-LAMP1 antibody [EPR21026] - Lysosome Marker (AB237307)

関連する標識済み抗体及び組成の異なる製品 (4)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

標識

励起波長/蛍光波長

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (6)

組合せ製品

Assay Kits

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Abcam product promise