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AB214944

Alexa Fluor® 647 Anti-ICAM1 抗体 [EPR4776]

Alexa Fluor® 647 Anti-ICAM1 antibody [EPR4776]

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(3 Publications)

Rabbit Recombinant Monoclonal ICAM1 antibody - conjugated to Alexa Fluor® 647. Suitable for ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human samples. Cited in 3 publications.

別名を表示する

CD54, Intercellular adhesion molecule 1, ICAM-1, Major group rhinovirus receptor, ICAM1

1 Images
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-ICAM1 antibody [EPR4776] (AB214944)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-ICAM1 antibody [EPR4776] (AB214944)

ab214944 staining ICAM1 in Raji cells. The cells were fixed with 4% formaldehyde (10 min) and then incubated in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h to permeabilize the cells and block non-specific protein-protein interactions. The cells were then incubated overnight at +4°C with ab214944 at 1/100 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

This product also gave a positive signal under the same testing conditions in Raji cells fixed with 80% methanol (5 min).

関連する標識済み抗体及び組成の異なる製品 (3)

  • Biotin

    Biotin Anti-ICAM1 antibody [EPR4776]

  • Unconjugated

    Anti-ICAM1 antibody [EPR4776]

  • Carrier free

    Anti-ICAM1 antibody [EPR4776] - BSA and Azide free

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR4776

アイソタイプ

IgG

標識

Alexa Fluor® 647

励起波長/蛍光波長

Ex: 650nm, Em: 665nm

キャリアフリー

No

交差種

Human, Human

アプリケーション

ICC/IF, IHC-P, Flow Cyt (Intra)

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p>This product gave a positive signal in Raji cells fixed with 4% formaldehyde (10 min) and 80% methanol (5 min)</p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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製品の詳細

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle|Store in the dark
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Cell adhesion molecule that functions as a receptor ligand of the signaling receptor ITGAL : ITGB2/LFA-1 (lymphocyte-function associated (LFA) molecule 1) ensuring leukocyte cell-cell adhesion, by providing a calibrated system to namely adjust T-cell killing to the antigen stimulation strength (PubMed : 3086451, PubMed : 3340213, PubMed : 38195629). Also functions as a ligand receptor of the signaling receptor ITGAM : ITGB2/MAC-1 ensuring adhesion between stimulated neutrophils and stimulated endothelial cells (PubMed : 1980124). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation (PubMed : 17875742). Promotes cell aggregation in epithelial cells through interaction with MUC1 (PubMed : 11173916).. (Microbial infection) Acts as a receptor for major receptor group rhinovirus A-B capsid proteins.. (Microbial infection) Acts as a receptor for Coxsackievirus A21 capsid proteins.. (Microbial infection) Upon Kaposi's sarcoma-associated herpesvirus/HHV-8 infection, is degraded by viral E3 ubiquitin ligase MIR2, presumably to prevent lysis of infected cells by cytotoxic T-lymphocytes and NK cell.
See full target information ICAM1
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文献 (3)

Recent publications for all applications. Explore the full list and refine your search

Molecular metabolism 99:102211 PubMed40681103

2025

Mitochondrial-targeted plastoquinone therapy prevents early onset muscle weakness that occurs before atrophy during ovarian cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Luca J Delfinis,Shahrzad Khajehzadehshoushtar,Luke D Flewwelling,Nathaniel J Andrews,Madison C Garibotti,Shivam Gandhi,Aditya N Brahmbhatt,Brooke A Morris,Bianca Garlisi,Sylvia Lauks,Caroline Aitken,Leslie Ogilvie,Stavroula Tsitkanou,Jeremy A Simpson,Nicholas P Greene,Arthur J Cheng,Jim Petrik,Christopher G R Perry

Journal of pharmaceutical analysis 15:101251 PubMed40521370

2025

Impact of FASN-enriched EVs on endothelial cell function in obstructive sleep apnea hypopnea syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Yuan Tian,Dan Zhang,Huaian Yang,Xiaoli Zhang,Shengqun Xu

Biomedical engineering online 20:65 PubMed34193168

2021

Celastrol inhibits the proliferation and angiogenesis of high glucose-induced human retinal endothelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jian Fang,Xiaoke Chang
View all publications
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代替製品

Primary Antibodies

AB179707

Anti-ICAM1 antibody [EPR16608]

RabMAb|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAM1 antibody [EPR16608] (AB179707)

  • 5
  • 5
Primary Antibodies

AB53013

Anti-ICAM1 antibody [EP1442Y]

BOND RX™ Validated|RabMAb|Recombinant|Trial Size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAM1 antibody [EP1442Y] (AB53013)

  • 0
  • 0
Primary Antibodies

AB271933

Anti-ICAM1 antibody [EPR16608] - BSA and Azide free

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAM1 antibody [EPR16608] - BSA and Azide free (AB271933)

  • 0
  • 0
Primary Antibodies

AB271852

Anti-ICAM1 antibody [EP1442Y] - BSA and Azide free

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAM1 antibody [EP1442Y] - BSA and Azide free (AB271852)

  • 0
  • 0
Primary Antibodies

AB221777

Anti-ICAM1 antibody [EP1442Y] - Low endotoxin, Azide free

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAM1 antibody [EP1442Y] - Low endotoxin, Azide free (AB221777)

  • 0
  • 0
Primary Antibodies

AB222736

Anti-ICAM1 antibody [EPR22161-284]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAM1 antibody [EPR22161-284] (AB222736)

  • 0
  • 0
Primary Antibodies

AB206398

Anti-ICAM1 antibody [EPR19938]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAM1 antibody [EPR19938] (AB206398)

  • 4
  • 1

組合せ製品

Assay Kits

AB174445

Human ICAM1 ELISA Kit (CD54)

SimpleStep

ELISA - Human ICAM1 ELISA Kit (CD54) (AB174445)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB195540

HRP Anti-VCAM1 antibody [EPR5047]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-VCAM1 antibody [EPR5047] (AB195540)

  • 0
  • 0
Primary Antibodies

AB15580

Anti-Ki67 antibody

BOND RX™ Validated|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody (AB15580)

  • 5
  • 230
Primary Antibodies

AB200317

Alexa Fluor® 647 Anti-CD45 antibody [EP322Y]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-CD45 antibody [EP322Y] (AB200317)

  • 0
  • 1

Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。
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