Alexa Fluor® 647 Anti-Hsp60 抗体 [EPR18245-93] (ab303590)
![Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Hsp60 antibody [EPR18245-93] (ab303590)](https://www.abcam.co.jp/ps/products/303/ab303590/Images/ab303590-493878-anti-hsp60-antibody-epr1824593-a647-immunocytochemistry-hela2.jpg "Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Hsp60 antibody [EPR18245-93] (ab303590)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Alexa Fluor® 647 Rabbit monoclonal [EPR18245-93] to Hsp60
- Suitable for: Flow Cyt (Intra), ICC/IF
- Reacts with: Human
- Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm
Related conjugates and formulations
製品の概要
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製品名
Alexa Fluor® 647 Anti-Hsp60 antibody [EPR18245-93]
Hsp60 一次抗体 製品一覧 -
製品の詳細
Alexa Fluor® 647 Rabbit monoclonal [EPR18245-93] to Hsp60 -
由来種
Rabbit -
標識
Alexa Fluor® 647. Ex: 652nm, Em: 668nm -
アプリケーション
適用あり: Flow Cyt (Intra), ICC/IFmore details -
種交差性
交差種: Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- Flow Cyt (Intra): HeLa cells. ICC/IF: HeLa cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA -
Concentration information loading... -
ポリ/モノ
モノクローナル -
クローン名
EPR18245-93 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab303590の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Flow Cyt (Intra) |
1/12500.
|
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| ICC/IF |
Use a concentration of 0.04 µg/ml.
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| 特記事項 |
|---|
|
Flow Cyt (Intra)
1/12500. |
|
ICC/IF
Use a concentration of 0.04 µg/ml. |
ターゲット情報
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機能
Implicated in mitochondrial protein import and macromolecular assembly. May facilitate the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix. -
関連疾患
Defects in HSPD1 are a cause of spastic paraplegia autosomal dominant type 13 (SPG13) [MIM:605280]. Spastic paraplegia is a degenerative spinal cord disorder characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs.
Defects in HSPD1 are the cause of leukodystrophy hypomyelinating type 4 (HLD4) [MIM:612233]; also called mitochondrial HSP60 chaperonopathy or MitCHAP-60 disease. HLD4 is a severe autosomal recessive hypomyelinating leukodystrophy. Clinically characterized by infantile-onset rotary nystagmus, progressive spastic paraplegia, neurologic regression, motor impairment, profound mental retardation. Death usually occurrs within the first two decades of life. -
配列類似性
Belongs to the chaperonin (HSP60) family. -
細胞内局在
Mitochondrion matrix. - Information by UniProt
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参照データベース
- Entrez Gene: 3329 Human
- Omim: 118190 Human
- SwissProt: P10809 Human
- Unigene: 595053 Human
- Unigene: 727543 Human
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別名
- 60 kDa chaperonin antibody
- 60 kDa heat shock protein, mitochondrial antibody
- CH60_HUMAN antibody
see all
画像
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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Hsp60 antibody [EPR18245-93] (ab303590)
AB303590 staining HSP60 in HeLa cells. The cells were fixed with 100% methanol (5 min at -20°C), permeabilized with 0.1% Triton x-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3 M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with AB303590 at 0.04 µg/ml (shown in red) and ab195883 (Alexa Fluor®488 Anti-Tubulin antibody [YOL1/34]) at 5 µg/ml (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). Image was acquired with a high-content analyzer (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
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![Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-Hsp60 antibody [EPR18245-93] (ab303590)](https://www.abcam.co.jp/ps/products/303/ab303590/Images/ab303590-2-anti-hsp60-antibody-epr18245-93-a647-hela-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-Hsp60 antibody [EPR18245-93] (ab303590)Flow cytometry overlay histogram showing HeLa cells stained with AB303590 (red line). The cells were fixed with 80 % methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were incubated in 1x PBS containing 10 % normal goat serum to block non-specific protein-protein interaction followed by the antibody (AB303590) (1x 106 in 100μl at 0.04μg/ml (1/12500)) for 30 min at 22°C.
Isotype control antibody (black line) was Rabbit IgG (monoclonal) Alexa Fluor® 647 (ab199093) used at the same concentration and conditions as the primary antibody. Unlabeled sample (blue line) was also used as a control.
Acquisition of >5000 events were collected using a 40 mW Red laser (638 nm) and 660/10 bandpass filter.
This antibody gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min) / permeabilized with 0.1% PBS-Triton X-100 for 15 min used under the same conditions.
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![Alexa Fluor® 647 Anti-Hsp60 antibody [EPR18245-93] (ab303590)](https://www.abcam.co.jp/ps/products/303/ab303590/Images/ab303590-494247-MTOConjugatedRecombinantAntibodyBenefits.jpg)
Alexa Fluor® 647 Anti-Hsp60 antibody [EPR18245-93] (ab303590) -
![Alexa Fluor® 647 Anti-Hsp60 antibody [EPR18245-93] (AB303590)](https://www.abcam.co.jp/ps/products/303/ab303590/Images/AB303590-1-Benefits-of-Recombinant-antibodies.jpg)
Alexa Fluor® 647 Anti-Hsp60 antibody [EPR18245-93] (AB303590)
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (0)
ab303590 は論文での使用が確認できていません。