Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
リコンビナントRabMAb

Alexa Fluor® 647 Anti-HA tag 抗体 [EPR22819-101] (ab288325)

Submit a review Submit a question
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-HA tag antibody [EPR22819-101] (ab288325)
  • Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-HA tag antibody [EPR22819-101] (ab288325)
  • Alexa Fluor® 647 Anti-HA tag antibody [EPR22819-101] (ab288325)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Alexa Fluor® 647 Rabbit monoclonal [EPR22819-101] to HA tag
  • Suitable for: ICC, Flow Cyt (Intra)
  • Reacts with: Species independent
  • Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm

Conjugates logo Related conjugates and formulations

FITC Unconjugated

製品の概要

  • 製品名

    Alexa Fluor® 647 Anti-HA tag antibody [EPR22819-101]
    HA tag 一次抗体 製品一覧

  • 製品の詳細

    Alexa Fluor® 647 Rabbit monoclonal [EPR22819-101] to HA tag

  • 由来種

    Rabbit

  • 標識

    Alexa Fluor® 647. Ex: 652nm, Em: 668nm

  • アプリケーション

    適用あり: ICC, Flow Cyt (Intra)more details

  • 種交差性

    交差種: Species independent

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • ICC: HEK293T transfected with HA tagged expression construct cells Flow Cyt (Intra): HEK-293T (human embryonic kidney) transfected with TATA-box-binding protein (WT) expression vector containing HA-tag positive cells

  • 特記事項

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.   

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark.

  • バッファー

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPR22819-101

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab288325の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ICC
Use a concentration of 2 µg/ml.
Flow Cyt (Intra)
1/5000.
特記事項
ICC
Use a concentration of 2 µg/ml.
Flow Cyt (Intra)
1/5000.

ターゲット情報

  • 関連性

    Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.

  • 別名

    • HA epitope tag antibody
    • HA1 antibody
    • HA2 antibody
    • hemagglutinin antibody
    • Hemagglutinin HA1 chain antibody
    • Hemagglutinin HA2 chain antibody
    see all

画像

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-HA tag antibody [EPR22819-101] (ab288325)
    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-HA tag antibody [EPR22819-101] (ab288325)

    ab288325 staining HA tag in HEK293T transfected with HA tagged expression contruct cells. The cells were fixed with 4% formaldehyde (10 min), permeabilised with 0.1% Triton x-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab288325 at 2 µg/ml (shown in red). ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488) (shown in green) was used at 2.5 µg/ml for structural counterstaining.

    Image was acquired with a confocal microscope (Leica-Microsystems TCS SP8) and a single confocal section is shown.

  • Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-HA tag antibody [EPR22819-101] (ab288325)
    Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-HA tag antibody [EPR22819-101] (ab288325)

    Flow cytometry overlay histogram showing right HEK-293T (human embryonic kidney) transfected with TATA-box-binding protein (WT) expression vector containing HA-tag positive cells and left negative HEK293T cells stained with ab288325 (red line).

    The cells were fixed with 4 % formaldehyde (10 min) and then permeabilized with 90% Methanol. The cells were then incubated in 1x PBS containing 10 % normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab288325) (1x 106 in 100 μl at 0.1 μg/ml (1/5000)) for 30 min at 22°C. 

  • Alexa Fluor® 647 Anti-HA tag antibody [EPR22819-101] (ab288325)
    Alexa Fluor® 647 Anti-HA tag antibody [EPR22819-101] (ab288325)

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (0)

ab288325 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab288325 は論文での使用が確認できていません。

レビューと Q&A

Show All レビュー Q&A
レビューを送る 質問を送る

There are currently no Customer reviews or Questions for ab288325.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com