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AB315362

Alexa Fluor® 647 Anti-GPCR LGR6 抗体 [EPR6874]

Alexa Fluor® 647 Anti-GPCR LGR6 antibody [EPR6874]

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Rabbit Recombinant Monoclonal GPCR LGR6 antibody - conjugated to Alexa Fluor® 647. Suitable for IHC-P, Flow Cyt (Intra), ICC/IF and reacts with Human, Mouse, Rat samples.

別名を表示する

UNQ6427/PRO21331, VTS20631, LGR6, Leucine-rich repeat-containing G-protein coupled receptor 6

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-GPCR LGR6 antibody [EPR6874] (AB315362)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-GPCR LGR6 antibody [EPR6874] (AB315362)

Immunohistochemical analysis of paraffin-embedded Human lung cancer tissue labeling GPCR LGR6 with ab315362 at 1/50 (10.0 ug/ml) dilution.

Cytoplasmic staining on human lung cancer. The section was incubated with ab315362 at 4°C overnight (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-GPCR LGR6 antibody [EPR6874] (AB315362)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-GPCR LGR6 antibody [EPR6874] (AB315362)

Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling GPCR LGR6 with ab315362 at 1/50 (10.0 ug/ml) dilution.

Cytoplasmic staining on rat liver. The section was incubated with ab315362 at 4°C overnight (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-GPCR LGR6 antibody [EPR6874] (AB315362)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-GPCR LGR6 antibody [EPR6874] (AB315362)

Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling GPCR LGR6 with ab315362 at 1/50 (10.0 ug/ml) dilution.

Cytoplasmic staining on mouse liver. The section was incubated with ab315362 at 4°C overnight (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0).

関連する標識済み抗体及び組成の異なる製品 (4)

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    Anti-GPCR LGR6 antibody [EPR6874]

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    Alexa Fluor® 488 Anti-GPCR LGR6 antibody [EPR6874]

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    PE Anti-GPCR LGR6 antibody [EPR6874]

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR6874

アイソタイプ

IgG

標識

Alexa Fluor® 647

励起波長/蛍光波長

Ex: 650nm, Em: 665nm

キャリアフリー

No

交差種

Human, Mouse, Rat

アプリケーション

ICC/IF, Flow Cyt (Intra), IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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組合せ製品

Reagents, Controls & Accessories

AB119211

InstantBlue® Coomassie Protein Stain (ISB1L)

Western blot - InstantBlue® Coomassie Protein Stain (ISB1L) (AB119211)

  • 5
  • 5
Primary Antibodies

AB6326

Anti-BrdU antibody [BU1/75 (ICR1)] - Proliferation Marker

Flow Cytometry (Intracellular) - Anti-BrdU antibody [BU1/75 (ICR1)] - Proliferation Marker (AB6326)

  • 5
  • 104
Primary Antibodies

AB80592

Anti-Ku80 antibody [EPR3468]

20ul selling size|RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-Ku80 antibody [EPR3468] (AB80592)

  • 0
  • 0
Primary Antibodies

AB213193

PE Anti-Cytokeratin 7 antibody [EPR17078]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - PE Anti-Cytokeratin 7 antibody [EPR17078] (AB213193)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50", "IHCP-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50", "IHCP-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50", "IHCP-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
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製品の詳細

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle|Store in the dark
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

GPCR LGR6 also known as leucine-rich repeat-containing G protein-coupled receptor 6 is a transmembrane receptor that belongs to the large GPCR family. It has a molecular weight of approximately 98 kDa. LGR6 expresses in various tissues including skin bone and certain regions of the brain. The receptor binds specific ligands which initiates signal transduction pathways that mediate various cellular responses. This function places LGR6 as an important component in multiple physiological processes.
Biological function summary

LGR6 plays an important role in signaling pathways involved in stem cell maintenance and tissue regeneration. It functions together with other proteins as part of a larger receptor complex. In the skin LGR6 marks a population of stem cells involved in hair follicle cycling and epidermal renewal. Its activity influences the cellular communication required for tissue homeostasis and repair. This receptor helps in determining cellular fate and proliferation in various tissues.

Pathways

LGR6 participates in the Wnt signaling pathway which is important for cell differentiation growth and migration. LGR6 interacts with proteins such as R-spondins enhancing Wnt signaling. This connection highlights LGR6's role in modulating pathways essential for embryonic development and tissue patterning. The interaction with Wnt and related proteins positions LGR6 as a modulator of pathways that govern stem cell behavior.

Abnormalities in LGR6 function have associations with skin disorders and cancers. Mutations or dysregulation of LGR6 can lead to impaired wound healing and contribute to pathological conditions. In cancer LGR6 has connections with proteins that affect tumor growth and metastasis. Studies suggest involvement of LGR6 in pathways related to squamous cell carcinoma further linking it with proteins involved in cancer progression therefore underlining its potential as a therapeutic target.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Receptor for R-spondins that potentiates the canonical Wnt signaling pathway and acts as a marker of multipotent stem cells in the epidermis. Upon binding to R-spondins (RSPO1, RSPO2, RSPO3 or RSPO4), associates with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. In contrast to classical G-protein coupled receptors, does not activate heterotrimeric G-proteins to transduce the signal. May act as a tumor suppressor.
See full target information LGR6
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Abcam product promise

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