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リコンビナントRabMAb

Alexa Fluor® 647 Anti-Glutamine Synthetase 抗体 [EPR13022(B)] (ab300751)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Glutamine Synthetase antibody [EPR13022(B)] (AB300751)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Glutamine Synthetase antibody [EPR13022(B)] (AB300751)
  • Alexa Fluor® 647 Anti-Glutamine Synthetase antibody [EPR13022(B)] (AB300751)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Alexa Fluor® 647 Rabbit monoclonal [EPR13022(B)] to Glutamine Synthetase
  • Suitable for: IHC-P
  • Reacts with: Human
  • Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Carrier Free HRP Unconjugated

製品の概要

  • 製品名

    Alexa Fluor® 647 Anti-Glutamine Synthetase antibody [EPR13022(B)]
    Glutamine Synthetase 一次抗体 製品一覧

  • 製品の詳細

    Alexa Fluor® 647 Rabbit monoclonal [EPR13022(B)] to Glutamine Synthetase

  • 由来種

    Rabbit

  • 標識

    Alexa Fluor® 647. Ex: 652nm, Em: 668nm

  • アプリケーション

    適用あり: IHC-Pmore details
    適用なし: IHC-Fr

  • 種交差性

    交差種: Human
    非交差種: Mouse, Rat

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • IHC-P: Human glioma; human breast tumor FFPE tissue sections.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab300751の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
特記事項
IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
追加情報
Is unsuitable for IHC-Fr.

ターゲット情報

  • 機能

    This enzyme has 2 functions: it catalyzes the production of glutamine and 4-aminobutanoate (gamma-aminobutyric acid, GABA), the latter in a pyridoxal phosphate-independent manner (By similarity). Essential for proliferation of fetal skin fibroblasts.

  • 関連疾患

    Defects in GLUL are the cause of congenital systemic glutamine deficiency (CSGD) [MIM:610015]. CSGD is a rare developmental disorder with severe brain malformation resulting in multi-organ failure and neonatal death. Glutamine is largely absent from affected patients serum, urine and cerebrospinal fluid.

  • 配列類似性

    Belongs to the glutamine synthetase family.

  • 発生段階

    Expressed during early fetal stages.

  • 細胞内局在

    Cytoplasm. Mitochondrion.
  • Information by UniProt

  • 参照データベース

    • 別名

      • cell proliferation-inducing protein 59 antibody
      • Cgl2214 antibody
      • GLNA antibody
      • GLNA_HUMAN antibody
      • GLNS antibody
      • GLUL antibody
      • Glutamate ammonia ligase antibody
      • Glutamate decarboxylase antibody
      • Glutamate--ammonia ligase antibody
      • glutamine synthase antibody
      • Glutamine synthetase antibody
      • glutamine synthetase I antibody
      • GS antibody
      • PIG 43 antibody
      • PIG 59 antibody
      • PIG43 antibody
      • PIG59 antibody
      • Proliferation inducing protein 43 antibody
      see all

    画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Glutamine Synthetase antibody [EPR13022(B)] (AB300751)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Glutamine Synthetase antibody [EPR13022(B)] (AB300751)

      Immunohistochemical analysis of paraffin-embedded human breast tumor tissue labeling Glutamine Synthetase with ab300751 at 1/100 (5.0 µg/ml). Positive cytoplasm staining on human breast tumor. The section was incubated with ab300751 for 60 mins at room temperature (shown in red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND™ RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Counterstained with DAPI.

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 40 mins

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Glutamine Synthetase antibody [EPR13022(B)] (AB300751)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Glutamine Synthetase antibody [EPR13022(B)] (AB300751)

      Immunohistochemical analysis of paraffin-embedded human glioma tissue labeling Glutamine Synthetase with ab300751 at 1/100 (5.0 µg/ml). Positive cytoplasmic staining on human glioma. The section was incubated with ab300751 for 60 mins at room temperature (shown in red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND™ RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Counterstained with DAPI.

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 40 mins

    • Alexa Fluor® 647 Anti-Glutamine Synthetase antibody [EPR13022(B)] (AB300751)
      Alexa Fluor® 647 Anti-Glutamine Synthetase antibody [EPR13022(B)] (AB300751)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab300751 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab300751 は論文での使用が確認できていません。

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