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AB215949

Alexa Fluor® 647 Anti-GFPT2 抗体 [EPR19095]

Alexa Fluor® 647 Anti-GFPT2 antibody [EPR19095]

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(1 Publication)

Rabbit Recombinant Monoclonal GFPT2 antibody - conjugated to Alexa Fluor® 647. Suitable for ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

別名を表示する

Glutamine--fructose-6-phosphate aminotransferase [isomerizing] 2, D-fructose-6-phosphate amidotransferase 2, Glutamine:fructose-6-phosphate amidotransferase 2, Hexosephosphate aminotransferase 2, GFAT 2, GFAT2, GFPT2

1 Images
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-GFPT2 antibody [EPR19095] (AB215949)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-GFPT2 antibody [EPR19095] (AB215949)

ab215949 staining GFPT2 in U87MG cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab215949 at 1/100 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

This product also gave a positive signal under the same testing conditions in U87MG cells fixed with 100% methanol (5 min).

関連する標識済み抗体及び組成の異なる製品 (3)

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR19095

アイソタイプ

IgG

標識

Alexa Fluor® 647

励起波長/蛍光波長

Ex: 650nm, Em: 665nm

キャリアフリー

No

交差種

Human, Human, Mouse, Rat

アプリケーション

ICC/IF, Flow Cyt (Intra)

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p>This product gave a positive signal in U87MG cells fixed with 4% formaldehyde (10 min) and 100% methanol (5 min)</p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" }, "Rat": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" } } }
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製品の詳細

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle|Store in the dark
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Controls the flux of glucose into the hexosamine pathway. Most likely involved in regulating the availability of precursors for N- and O-linked glycosylation of proteins.
See full target information Glutamine--fructose-6-phosphate aminotransferase [isomerizing] 2
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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Aging 11:1759-1777 PubMed30913540

2019

Tubastatin A inhibits HDAC and Sirtuin activity rather than being a HDAC6-specific inhibitor in mouse oocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Yun-Jung Choi,Min-Hee Kang,Kwonho Hong,Jin-Hoi Kim
View all publications
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代替製品

Primary Antibodies

AB155926

Anti-GFPT2 antibody

Western blot - Anti-GFPT2 antibody (AB155926)

  • 0
  • 0

組合せ製品

Assay Kits

AB197011

Glutamine Assay Kit (Colorimetric)

Functional Studies - Glutamine Assay Kit (Colorimetric) (AB197011)

  • 3
  • 1
Primary Antibodies

AB225314

Alexa Fluor® 488 Anti-GFP antibody [EPR14104] - GFP booster

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-GFP antibody [EPR14104] - GFP booster (AB225314)

  • 2
  • 2
Primary Antibodies

AB234847

Anti-TRIM13 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIM13 antibody (AB234847)

  • 0
  • 0
Primary Antibodies

AB177941

Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody [EPR12713]

BOND RX™ Validated|RabMAb|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody [EPR12713] (AB177941)

  • 5
  • 3
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20

Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。
保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com