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リコンビナントRabMAb

Alexa Fluor® 647 Anti-Frataxin 抗体 [EPR21840] (ab303580)

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Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-Frataxin antibody [EPR21840] (ab303580)
  • Alexa Fluor® 647 Anti-Frataxin antibody [EPR21840] (ab303580)
  • Alexa Fluor® 647 Anti-Frataxin antibody [EPR21840] (AB303580)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Alexa Fluor® 647 Rabbit monoclonal [EPR21840] to Frataxin
  • Suitable for: Flow Cyt (Intra)
  • Reacts with: Human
  • Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm

Conjugates logo Related conjugates and formulations

Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Carrier Free PE Unconjugated

製品の概要

  • 製品名

    Alexa Fluor® 647 Anti-Frataxin antibody [EPR21840]
    Frataxin 一次抗体 製品一覧

  • 製品の詳細

    Alexa Fluor® 647 Rabbit monoclonal [EPR21840] to Frataxin

  • 由来種

    Rabbit

  • 標識

    Alexa Fluor® 647. Ex: 652nm, Em: 668nm

  • アプリケーション

    適用あり: Flow Cyt (Intra)more details

  • 種交差性

    交差種: Human

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • Flow Cyt (Intra): HCT-116 cells.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab303580の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt (Intra)
1/2500.
特記事項
Flow Cyt (Intra)
1/2500.

ターゲット情報

  • 機能

    Promotes the biosynthesis of heme and assembly and repair of iron-sulfur clusters by delivering Fe(2+) to proteins involved in these pathways. May play a role in the protection against iron-catalyzed oxidative stress through its ability to catalyze the oxidation of Fe(2+) to Fe(3+); the oligomeric form but not the monomeric form has in vitro ferroxidase activity. May be able to store large amounts of iron in the form of a ferrihydrite mineral by oligomerization; however, the physiological relevance is unsure as reports are conflicting and the function has only been shown using heterologous overexpression systems. Modulates the RNA-binding activity of ACO1.

  • 組織特異性

    Expressed in the heart, peripheral blood lymphocytes and dermal fibroblasts.

  • 関連疾患

    Defects in FXN are the cause of Friedreich ataxia (FRDA) [MIM:229300]. FRDA is an autosomal recessive, progressive degenerative disease characterized by neurodegeneration and cardiomyopathy it is the most common inherited ataxia. The disorder is usually manifest before adolescence and is generally characterized by incoordination of limb movements, dysarthria, nystagmus, diminished or absent tendon reflexes, Babinski sign, impairment of position and vibratory senses, scoliosis, pes cavus, and hammer toe. In most patients, FRDA is due to GAA triplet repeat expansions in the first intron of the frataxin gene. But in some cases the disease is due to mutations in the coding region.

  • 配列類似性

    Belongs to the frataxin family.

  • 翻訳後修飾

    Processed in two steps by mitochondrial processing peptidase (MPP). MPP first cleaves the precursor to intermediate form and subsequently converts the intermediate to yield frataxin mature form (frataxin(81-210)) which is the predominant form. The additional forms, frataxin(56-210) and frataxin(78-210), seem to be produced when the normal maturation process is impaired; their physiological relevance is unsure.

  • 細胞内局在

    Cytoplasm. Mitochondrion. PubMed:18725397 reports localization exclusively in mitochondria.
  • Information by UniProt

  • 参照データベース

    • 別名

      • CyaY antibody
      • d-FXN antibody
      • FA antibody
      • FARR antibody
      • Frataxin mature form antibody
      • Frataxin(81-210) antibody
      • FRDA antibody
      • FRDA_HUMAN antibody
      • Friedreich ataxia protein antibody
      • Fxn antibody
      • i-FXN antibody
      • m56-FXN antibody
      • m78-FXN antibody
      • m81-FXN antibody
      • MGC57199 antibody
      • X25 antibody
      see all

    画像

    • Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-Frataxin antibody [EPR21840] (ab303580)
      Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-Frataxin antibody [EPR21840] (ab303580)

      Flow cytometry overlay histogram showing HCT-116 cells stained with AB303580 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interaction followed by the antibody (AB303580) (1x 106 in 100μl at 0.2μg/ml (1/2500)) for 30 min at 22°C.

      Isotype control antibody (black line) was Rabbit IgG (monoclonal) Alexa Fluor® 647 (ab199093) used at the same concentration and conditions as the primary antibody. Unlabeled sample (blue line) was also used as a control.

      Acquisition of >5000 events were collected using a 40 mW Red laser (638 nm) and 660/10 bandpass filter.

    • Alexa Fluor® 647 Anti-Frataxin antibody [EPR21840] (ab303580)
      Alexa Fluor® 647 Anti-Frataxin antibody [EPR21840] (ab303580)
    • Alexa Fluor® 647 Anti-Frataxin antibody [EPR21840] (AB303580)
      Alexa Fluor® 647 Anti-Frataxin antibody [EPR21840] (AB303580)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab303580 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab303580 は論文での使用が確認できていません。

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