AB237396

Alexa Fluor® 647 Anti-EpCAM 抗体 [EPR20532-225]

Alexa Fluor® 647 Anti-EpCAM antibody [EPR20532-225]

RabMAb
Recombinant
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(3 Publications)

Anti-EpCAM antibody [EPR20532-225] - Alexa Fluor® 647 conjugated (ab237396) is a rabbit recombinant monoclonal antibody detecting EpCAM in Flow Cytometry, ICC/IF. Suitable for Human.

- Biophysical QC for unrivalled batch-batch consistency

別名を表示する

CD326, GA733-2, M1S2, M4S1, MIC18, TACSTD1, TROP1, EPCAM, Epithelial cell adhesion molecule, Ep-CAM, Adenocarcinoma-associated antigen, Cell surface glycoprotein Trop-1, Epithelial cell surface antigen, Epithelial glycoprotein, Epithelial glycoprotein 314, KS 1/4 antigen, KSA, Major gastrointestinal tumor-associated protein GA733-2, Tumor-associated calcium signal transducer 1, EGP, EGP314, hEGP314

2 Images

Flow Cytometry - Alexa Fluor® 647 Anti-EpCAM antibody [EPR20532-225] (AB237396)

Flow Cyt

Unknown

Flow Cytometry - Alexa Fluor® 647 Anti-EpCAM antibody [EPR20532-225] (AB237396)

Overlay histogram showing HT-29 cells stained with ab237396 (red line).

The cells were incubated in 1x PBS/10% normal Goat serum to block non-specific protein-protein interactions followed by the antibody (ab237396) (1x10⁶ in 100 μl at 1 μg/ml (1/500)) for 30 min at 22°C.

Isotype control antibody (black line) was Rabbit IgG (monoclonal) Alexa Fluor^® 647 (ab199093) used at the same concentration and conditions as the primary antibody. Unlabeled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 40 mW Red laser (640nm) and 670/14 bandpass filter.

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-EpCAM antibody [EPR20532-225] (AB237396)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-EpCAM antibody [EPR20532-225] (AB237396)

ab237396 staining EpCAM in HT-29 cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab237396 at 1/100 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor^® 488), at 1/250 dilution (shown in ). Nuclear DNA was labeled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

This product also gave a positive signal under the same testing conditions in HT-29 cells fixed with 100% methanol (5 min).

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR20532-225

アイソタイプ

IgG

標識

Alexa Fluor® 647

励起波長/蛍光波長

Ex: 650nm, Em: 665nm

キャリアフリー

交差種

Human

アプリケーション

ICC/IF, Flow Cyt

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/500", "FlowCyt-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p>This product gave a positive signal in HT-29 fixed with 4% formaldehyde (10 min) and 100% methanol (5 min).</p>" } } }

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製品の詳細

What is this antibody validated in?
Alexa Fluor® 647 Anti-EpCAM antibody [EPR20532-225] (ab237396) is a rabbit recombinant monoclonal antibody and is validated for use in Flow Cytometry (Flow Cyt), Immunocytochemistry/immunofluorescence (ICC/IF) in Human samples.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Related products
Antibody clone EPR20532-225 is also available pre-conjugated to a variety of labels for your convenience – Anti-EpCAM Alexa Fluor® 647 [EPR20532-225] (ab237396).

Other related products
We have a range of other formats of antibody clone [EPR20532-225] also available for your convenience: ab223582, Carrier free - ab225894, Alexa Fluor® 488 - ab237395, Alexa Fluor® 647 - ab237396, PE - ab237397, APC - ab237398, Alexa Fluor® 594 - ab286811, Biotin - ab314940

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle|Stable for 12 months at -20°C|Store in the dark

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

EpCAM also known as epithelial cell adhesion molecule is a transmembrane glycoprotein with a molecular weight of around 40 kDa. Alternative names include CD326 and TACSTD1. This protein is expressed on the surface of most epithelial cells and plays a role in cell adhesion. It is involved in maintaining the integrity of epithelial tissues by mediating homotypic cell-cell interactions which are important for the structure and function of tissues.

Biological function summary

EpCAM facilitates signaling pathways that impact cellular processes like proliferation and differentiation. It is not a part of a larger protein complex but interacts directly with other cellular machinery to transmit intracellular signals. EpCAM influences stem cell behavior and is important in tissue homeostasis assisting in both normal regenerative processes and wound healing.

Pathways

EpCAM participates in regulatory mechanisms involving the Wnt/Β-catenin signaling pathway which affects gene expression and cell fate decisions. Through its action EpCAM modulates components like E-cadherin which is important for cell adhesion and the maintenance of the epithelial layer. It indirectly influences pathways relating to MYC a transcription factor that regulates cell cycle progression and apoptosis.

EpCAM is associated with various types of cancer including colorectal and breast cancer. Its overexpression often correlates with aggressive cancer phenotypes and poor prognosis. Besides cancers EpCAM mutations can lead to congenital tufting enteropathy a rare condition affecting intestinal epithelial structure and function. Aberrant EpCAM expression affects Β-catenin levels impacting cellular pathways linked to tumor progression and epithelial integrity.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

May act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Plays a role in embryonic stem cells proliferation and differentiation. Up-regulates the expression of FABP5, MYC and cyclins A and E.

See full target information EPCAM

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文献 (3)

Recent publications for all applications. Explore the full list and refine your search

Evolution, medicine, and public health 13:281-290 PubMed41069959

2025

Dual targeting of conserved cell cycle and transcription programs in advanced colorectal cancer by fadraciclib.

Applications

Unspecified application

Species

Unspecified reactive species

Mohammad Zokaasadi,Wylie K Watlington,Divya L Dayanidhi,John B Mantyh,Gabrielle Rupprecht,Shannon McCall,David G Blake,Jason A Somarelli,David S Hsu

Nature immunology 26:582-594 PubMed40119192

2025

Enhancing human NK cell antitumor function by knocking out SMAD4 to counteract TGFβ and activin A suppression.

Applications

Unspecified application

Species

Unspecified reactive species

Anna Rea,Sara Santana-Hernández,Javier Villanueva,Marta Sanvicente-García,Mariona Cabo,Jesús Suarez-Olmos,Fabricio Quimis,Mengjuan Qin,Eduard Llorens,Sandra Blasco-Benito,Lamberto Torralba-Raga,Lorena Perez,Bishan Bhattarai,Elisenda Alari-Pahissa,Anna-Maria Georgoudaki,Francesc Balaguer,Manel Juan,Julián Pardo,Toni Celià-Terrassa,Ana Rovira,Nina Möker,Congcong Zhang,Marco Colonna,Jan Spanholtz,Karl-Johan Malmberg,Clara Montagut,Joan Albanell,Marc Güell,Miguel López-Botet,Aura Muntasell

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2303379 PubMed38380561

2024

Chromatin Remodeling in Patient-Derived Colorectal Cancer Models.

Applications

Unspecified application

Species

Unspecified reactive species

Kun Xiang,Ergang Wang,John Mantyh,Gabrielle Rupprecht,Marcos Negrete,Golshid Sanati,Carolyn Hsu,Peggy Randon,Anders Dohlman,Kai Kretzschmar,Shree Bose,Nicholas Giroux,Shengli Ding,Lihua Wang,Jorge Prado Balcazar,Qiang Huang,Pasupathi Sundaramoorthy,Rui Xi,Shannon Jones McCall,Zhaohui Wang,Chongming Jiang,Yubin Kang,Scott Kopetz,Gregory E Crawford,Steven M Lipkin,Xiao-Fan Wang,Hans Clevers,David Hsu,Xiling Shen

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Alexa Fluor® 647 Anti-EpCAM 抗体 [EPR20532-225]

Alexa Fluor® 647 Anti-EpCAM antibody [EPR20532-225]

Flow Cytometry - Alexa Fluor® 647 Anti-EpCAM antibody [EPR20532-225] (AB237396)

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-EpCAM antibody [EPR20532-225] (AB237396)

関連する標識済み抗体及び組成の異なる製品 (5)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

標識

励起波長/蛍光波長

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (3)

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Abcam product promise