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リコンビナントRabMAb

Alexa Fluor® 647 Anti-EGFR (deleted E746 + L747 + R748 + E749 + A750) 抗体 [SP111] (ab300476)

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-EGFR (deleted E746 + L747 + R748 + E749 + A750) antibody [SP111] (ab300476)
  • Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-EGFR (deleted E746 + L747 + R748 + E749 + A750) antibody [SP111] (ab300476)
  • Alexa Fluor® 647 Anti-EGFR (deleted E746 + L747 + R748 + E749 + A750) antibody [SP111] (ab300476)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Alexa Fluor® 647 Rabbit monoclonal [SP111] to EGFR (deleted E746 + L747 + R748 + E749 + A750)
  • Suitable for: Flow Cyt (Intra), ICC/IF
  • Reacts with: Human
  • Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm

Conjugates logo Related conjugates and formulations

Alexa Fluor® 555 Alexa Fluor® 594 Alexa Fluor® 750 APC PE Unconjugated

製品の概要

  • 製品名

    Alexa Fluor® 647 Anti-EGFR (deleted E746 + L747 + R748 + E749 + A750) antibody [SP111]
    EGFR 一次抗体 製品一覧

  • 製品の詳細

    Alexa Fluor® 647 Rabbit monoclonal [SP111] to EGFR (deleted E746 + L747 + R748 + E749 + A750)

  • 由来種

    Rabbit

  • 標識

    Alexa Fluor® 647. Ex: 652nm, Em: 668nm

  • アプリケーション

    適用あり: Flow Cyt (Intra), ICC/IFmore details

  • 種交差性

    交差種: Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • ICC/IF: HCC827 (human lung adenocarcinoma epithelial cell). Flow cyt (intra): HCC827 (human lung adenocarcinoma epithelial cell).

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab300476の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt (Intra)
1/500.
ICC/IF
1/50.
特記事項
Flow Cyt (Intra)
1/500.
ICC/IF
1/50.

ターゲット情報

  • 機能

    Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling. Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin.
    Isoform 2 may act as an antagonist of EGF action.

  • 組織特異性

    Ubiquitously expressed. Isoform 2 is also expressed in ovarian cancers.

  • 関連疾患

    Lung cancer
    Inflammatory skin and bowel disease, neonatal, 2

  • 配列類似性

    Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.
    Contains 1 protein kinase domain.

  • 翻訳後修飾

    Phosphorylation at Ser-695 is partial and occurs only if Thr-693 is phosphorylated. Phosphorylation at Thr-678 and Thr-693 by PRKD1 inhibits EGF-induced MAPK8/JNK1 activation. Dephosphorylation by PTPRJ prevents endocytosis and stabilizes the receptor at the plasma membrane. Autophosphorylation at Tyr-1197 is stimulated by methylation at Arg-1199 and enhances interaction with PTPN6. Autophosphorylation at Tyr-1092 and/or Tyr-1110 recruits STAT3. Dephosphorylated by PTPN1 and PTPN2.
    Monoubiquitinated and polyubiquitinated upon EGF stimulation; which does not affect tyrosine kinase activity or signaling capacity but may play a role in lysosomal targeting. Polyubiquitin linkage is mainly through 'Lys-63', but linkage through 'Lys-48', 'Lys-11' and 'Lys-29' also occurs. Deubiquitination by OTUD7B prevents degradation. Ubiquitinated by RNF115 and RNF126.
    Methylated. Methylation at Arg-1199 by PRMT5 stimulates phosphorylation at Tyr-1197.

  • 細胞内局在

    Secreted and Cell membrane. Endoplasmic reticulum membrane. Golgi apparatus membrane. Nucleus membrane. Endosome. Endosome membrane. Nucleus. In response to EGF, translocated from the cell membrane to the nucleus via Golgi and ER. Endocytosed upon activation by ligand. Colocalized with GPER1 in the nucleus of estrogen agonist-induced cancer-associated fibroblasts (CAF).
  • Information by UniProt

  • 参照データベース

    • 別名

      • Avian erythroblastic leukemia viral (v erb b) oncogene homolog antibody
      • Cell growth inhibiting protein 40 antibody
      • Cell proliferation inducing protein 61 antibody
      • EGF R antibody
      • EGFR antibody
      • EGFR_HUMAN antibody
      • Epidermal growth factor receptor (avian erythroblastic leukemia viral (v erb b) oncogene homolog) antibody
      • Epidermal growth factor receptor (erythroblastic leukemia viral (v erb b) oncogene homolog avian) antibody
      • Epidermal growth factor receptor antibody
      • erb-b2 receptor tyrosine kinase 1 antibody
      • ERBB antibody
      • ERBB1 antibody
      • Errp antibody
      • HER1 antibody
      • mENA antibody
      • NISBD2 antibody
      • Oncogen ERBB antibody
      • PIG61 antibody
      • Proto-oncogene c-ErbB-1 antibody
      • Receptor tyrosine protein kinase ErbB 1 antibody
      • Receptor tyrosine-protein kinase ErbB-1 antibody
      • SA7 antibody
      • Species antigen 7 antibody
      • Urogastrone antibody
      • v-erb-b Avian erythroblastic leukemia viral oncogen homolog antibody
      • wa2 antibody
      • Wa5 antibody
      see all

    画像

    • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-EGFR (deleted E746 + L747 + R748 + E749 + A750) antibody [SP111] (ab300476)
      Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-EGFR (deleted E746 + L747 + R748 + E749 + A750) antibody [SP111] (ab300476)

      Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HCC827 (human lung adenocarcinoma epithelial cell) cells labelling EGFR (deleted E746 + L747 + R748 + E749 + A750) with ab300476 at 1/50 dilution (10.0 µg/mL) (Red). Confocal image showing membranous staining in HCC827 cells. Negative control: A431 (PMID:19129184). ab195887 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 488) was used to counterstain tubulin at 1/200 dilution (2.5 µg/ml) (Green). The nuclear counterstain was DAPI (Blue).

    • Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-EGFR (deleted E746 + L747 + R748 + E749 + A750) antibody [SP111] (ab300476)
      Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-EGFR (deleted E746 + L747 + R748 + E749 + A750) antibody [SP111] (ab300476)

      Flow cytometric (Intracellular) analysis of 4% paraformaldehyde fixed and 90% methanol permeabilized A431 (human epidermoid carcinoma epithelial cell, Left) / HCC827 (human lung adenocarcinoma epithelial cell, Right) labelling EGFR (deleted E746 + L747 + R748 + E749 + A750) with ab300476 at 1:500 dilution (0.1 µg) (Red) compared with rabbit monoclonal isotype control - Alexa Fluor® 647 (Black) and cells without incubation with primary antibody and secondary antibody (Blue). Negative control: A431 (PMID:19129184).

    • Alexa Fluor® 647 Anti-EGFR (deleted E746 + L747 + R748 + E749 + A750) antibody [SP111] (ab300476)
      Alexa Fluor® 647 Anti-EGFR (deleted E746 + L747 + R748 + E749 + A750) antibody [SP111] (ab300476)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab300476 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab300476 は論文での使用が確認できていません。

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