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AB237239

Alexa Fluor® 647 Anti-Collagen III 抗体 [EPR17673]

Alexa Fluor® 647 Anti-Collagen III antibody [EPR17673]

5

(1 Review)

|

(1 Publication)

Rabbit Recombinant Monoclonal Collagen III antibody - conjugated to Alexa Fluor® 647. Suitable for ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

別名を表示する

Collagen alpha-1(III) chain, COL3A1

3 Images
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Collagen III antibody [EPR17673] (AB237239)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Collagen III antibody [EPR17673] (AB237239)

ab237239 staining Collagen III in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab237239 at 1/1000 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Collagen III antibody [EPR17673] (AB237239)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Collagen III antibody [EPR17673] (AB237239)

Immunofluorescent analysis of 4% Paraformaldehyde fixed 0.1% Triton X-100 permeabilized PC-12 (rat adrenal gland pheochromocytoma cells) labelling Collagen III with ab237239 at 1/50 (10.0 μg/ml). ab195887 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 488) was used as a counterstain at 1/200 dilution (2.5μg/ml). Confocal image showing cytoplasmic staining in PC-12 cell line and no staining in rat splenocytes (shown in magenta). The counterstain was observed in green. Nuclear DNA was labelled with DAPI (shown in blue).

Negative control : Rat splenocytes.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Collagen III antibody [EPR17673] (AB237239)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Collagen III antibody [EPR17673] (AB237239)

Immunofluorescent analysis of 4% Paraformaldehyde fixed 0.1% Triton X-100 permeabilized RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophages) labelling Collagen III with ab237239 at 1/50 (10.0 μg/ml). ab195887 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 488) was used as a counterstain at 1/200 dilution (2.5μg/ml). Confocal image showing cytoplasmic staining in RAW 264.7 cell line and no staining in mouse splenocytes (shown in magenta). The counterstain was observed in green. Nuclear DNA was labelled with DAPI (shown in blue).

Negative control : Mouse splenocytes.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

関連する標識済み抗体及び組成の異なる製品 (3)

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR17673

アイソタイプ

IgG

標識

Alexa Fluor® 647

励起波長/蛍光波長

Ex: 650nm, Em: 665nm

キャリアフリー

No

交差種

Human, Human, Mouse, Rat

アプリケーション

Flow Cyt (Intra), ICC/IF

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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組合せ製品

Assay Kits

AB222942

Total Collagen Assay Kit (Perchlorate-Free)

Functional Studies - Total Collagen Assay Kit (Perchlorate-Free) (AB222942)

  • 4
  • 1
Reagents, Controls & Accessories

AB246832

Picro-Sirius Red Solution

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Picro-Sirius Red Solution (AB246832)

  • 0
  • 0
Primary Antibodies

AB280968

Alexa Fluor® 647 Anti-Collagen I antibody [EPR7785]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Collagen I antibody [EPR7785] (AB280968)

  • 1
  • 1
Primary Antibodies

AB184675

Alexa Fluor® 488 Anti-alpha smooth muscle Actin antibody [1A4]

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-alpha smooth muscle Actin antibody [1A4] (AB184675)

  • 5
  • 8
Primary Antibodies

AB198934

Alexa Fluor® 647 Anti-Fibronectin antibody [F1]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Fibronectin antibody [F1] (AB198934)

  • 0
  • 0
Primary Antibodies

AB309502

Alexa Fluor® 488 Anti-Collagen IV antibody [EPR22911-127]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-Collagen IV antibody [EPR22911-127] (AB309502)

  • 5
  • 1
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/1000", "ICCIF-species-notes": "<p>This product gave a positive signal in HeLa cells fixed with 100% methanol (5 min)</p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" }, "Rat": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" } } }
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製品の詳細

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle|Stable for 12 months at -20°C|Store in the dark
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Collagen type III also known as COL3 and collagen III is a fibrillar protein with an approximate molecular mass of 138 kDa. This protein is a significant component of the extracellular matrix and is mainly expressed in connective tissues like skin lungs and vascular walls. You can also find it in developing tissues and fibrotic tissues reflecting its role during tissue growth and repair processes.
Biological function summary

Collagen type III contributes to maintaining the structural integrity of tissues. It forms a complex with collagen type I providing a scaffold that ensures mechanical strength and stability to tissues. This structural pairing of collagen type I and III highlights their cooperative function in supporting the extracellular matrix and regulating cell behavior in tissue repair and wound healing processes.

Pathways

Collagen type III is integral to the processes of tissue repair and fibrosis pathways. It interacts closely with proteins such as fibronectin and integrins contributing to the cellular adhesion and signaling pathways important for fibroblast function and migration. These interactions reflect its essential role in matrix assembly and stability influencing how cells respond to injury and repair damaged tissues.

Collagen type III mutations or dysregulation associate with vascular Ehlers-Danlos syndrome and keloids. In vascular Ehlers-Danlos syndrome defective collagen III compromises vessel wall integrity resulting in vascular fragility and rupture. In the context of keloids excessive collagen III accumulation in the skin illustrates its role in pathological fibrosis. Collagen type I is another protein that plays an important role in these conditions contributing along with collagen type III to the altered structural and functional properties observed in these disorders.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Collagen type III occurs in most soft connective tissues along with type I collagen. Involved in regulation of cortical development. Is the major ligand of ADGRG1 in the developing brain and binding to ADGRG1 inhibits neuronal migration and activates the RhoA pathway by coupling ADGRG1 to GNA13 and possibly GNA12.
See full target information COL3A1
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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Materials today. Bio 29:101246 PubMed39351489

2024

A bioengineered tumor matrix-based scaffold for the evaluation of melatonin efficacy on head and neck squamous cancer stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Julia López de Andrés,César Rodríguez-Santana,Laura de Lara-Peña,Gema Jiménez,Germaine Escames,Juan Antonio Marchal
View all publications
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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。
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