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AB309367

Alexa Fluor® 647 Anti-Collagen I 抗体 [EPR24331-53]

Alexa Fluor® 647 Anti-Collagen I antibody [EPR24331-53]

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Anti-Collagen I antibody [EPR24331-53] - Alexa Fluor® 647 conjugated (ab309367) is a rabbit recombinant monoclonal antibody detecting Collagen I in IHC-P, ICC/IF. Suitable for Mouse, Rat.

- Biophysical QC for unrivalled batch-batch consistency

別名を表示する

Cola1, Col1a1, Collagen alpha-1(I) chain, Alpha-1 type I collagen

3 Images
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Collagen I antibody [EPR24331-53] (AB309367)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Collagen I antibody [EPR24331-53] (AB309367)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized NIH/3T3 (mouse embryonic fibroblast) cells labelling Collagen I with ab309367 at 1/250 (2 ug/ml) dilution (Green). Confocal image showing cytoplasmic staining in NIH/3T3 cells. Negative control : Hepa1-6 (PMID : 27853186)Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). ab195887 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 488) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Collagen I antibody [EPR24331-53] (AB309367)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Collagen I antibody [EPR24331-53] (AB309367)

Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labelling Collagen I with ab309367 at 1/100 (5.0 µg/mL). Positive staining on mouse testis. The section was incubated with ab309367 for 60 mins at room temperature (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND RX instrument. The image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Collagen I antibody [EPR24331-53] (AB309367)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Collagen I antibody [EPR24331-53] (AB309367)

Immunohistochemical analysis of paraffin-embedded Rat testis tissue labelling Collagen I with ab309367 at 1/100 (5.0 µg/mL). Positive staining on rat testis. The section was incubated with ab309367 for 60 mins at room temperature (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND RX instrument. The image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins.

関連する標識済み抗体及び組成の異なる製品 (9)

  • Unconjugated

    Anti-Collagen I antibody [EPR24331-53]

  • Carrier free

    Anti-Collagen I antibody [EPR24331-53] - BSA and Azide free

  • 660 APC

    APC Anti-Collagen I antibody [EPR24331-53]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-Collagen I antibody [EPR24331-53]

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-Collagen I antibody [EPR24331-53]

  • 603 Alexa Fluor® 568

    Alexa Fluor® 568 Anti-Collagen I antibody [EPR24331-53]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-Collagen I antibody [EPR24331-53]

  • 775 Alexa Fluor® 750

    Alexa Fluor® 750 Anti-Collagen I antibody [EPR24331-53]

  • 578 PE

    PE Anti-Collagen I antibody [EPR24331-53]

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR24331-53

アイソタイプ

IgG

標識

Alexa Fluor® 647

励起波長/蛍光波長

Ex: 650nm, Em: 665nm

キャリアフリー

No

交差種

Mouse, Rat

アプリケーション

IHC-P, Flow Cyt (Intra), ICC/IF

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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組合せ製品

Assay Kits

AB222942

Total Collagen Assay Kit (Perchlorate-Free)

Functional Studies - Total Collagen Assay Kit (Perchlorate-Free) (AB222942)

  • 4
  • 1
Reagents, Controls & Accessories

AB246832

Picro-Sirius Red Solution

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Picro-Sirius Red Solution (AB246832)

  • 0
  • 0
Primary Antibodies

AB237239

Alexa Fluor® 647 Anti-Collagen III antibody [EPR17673]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Collagen III antibody [EPR17673] (AB237239)

  • 5
  • 1
Primary Antibodies

AB184675

Alexa Fluor® 488 Anti-alpha smooth muscle Actin antibody [1A4]

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-alpha smooth muscle Actin antibody [1A4] (AB184675)

  • 5
  • 8
Primary Antibodies

AB237287

Alexa Fluor® 647 Anti-Fibronectin antibody [EPR19241-46]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Fibronectin antibody [EPR19241-46] (AB237287)

  • 0
  • 0
Primary Antibodies

AB24982

Cy5 ® Anti-CD45.1 antibody [A20]

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" } } }
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製品の詳細

What is this antibody validated in?
Alexa Fluor® 647 Anti-Collagen I antibody [EPR24331-53] (ab309367) is a rabbit recombinant monoclonal antibody and is validated for use in Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Mouse, Rat samples.

Related products
Antibody clone EPR24331-53 is also available pre-conjugated to a variety of labels for your convenience – Anti-Collagen I Alexa Fluor® 647 [EPR24331-53] (ab309367).

Other related products
We have a range of other formats of antibody clone [EPR24331-53] also available for your convenience: ab270993, Carrier free - ab279711, Alexa Fluor® 488 - ab309248, Alexa Fluor® 647 - ab309367, APC - ab310848, PE - ab310918, Alexa Fluor® 568 - ab312943, Alexa Fluor® 555 - ab313153, Alexa Fluor® 594 - ab313351, Alexa Fluor® 750 - ab321535

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle|Store in the dark
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Collagen type I also called collagen I is a structural protein expressed mainly in connective tissues such as skin tendon bone and ligaments. It serves as an important component in providing mechanical strength and integrity to these tissues. Collagen I is a fibrillar collagen known for its triple-helix structure composed of two alpha-1 chains and one alpha-2 chain and has a molecular mass of approximately 300 kDa. Researchers often employ collagen western blot and collagen ELISA techniques for its detection. Collagen suppliers offer various collagen antibodies used in these assays to study its distribution and function.
Biological function summary

Collagen type I plays a central role in maintaining the extracellular matrix and supporting cellular environments. It interacts with other matrix proteins and cells forming complexes that help in tissue development and repair. Type I collagen is especially important in bone matrix working alongside minerals like hydroxyapatite to provide rigidity and support. Anti-collagen antibodies aid in studying its biological functions and interactions which are critical to understanding tissue dynamics.

Pathways

Collagen type I interacts with multiple signaling cascades involved in tissue remodeling and repair. It is a significant player in the TGF-Β pathway which regulates fibrosis and wound healing processes. In these pathways proteins such as fibronectin and integrins work in concert with collagen type I to orchestrate cellular responses to damage. Researchers often examine its role in these pathways to uncover therapeutic possibilities for disease interventions.

Collagen type I has strong connections to conditions like osteogenesis imperfecta and fibrosis. Mutations or irregularities in collagen I production can lead to osteogenesis imperfecta a genetic disorder characterized by brittle bones. In fibrosis excessive collagen deposition disrupts normal tissue architecture contributing to organ dysfunction. In both conditions type I collagen interacts with other proteins like matrix metalloproteinases which modulate its breakdown and remodeling highlighting its importance in disease pathology.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Type I collagen is a member of group I collagen (fibrillar forming collagen).
See full target information Col1a1
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Abcam product promise

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