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AB205426

Alexa Fluor® 647 Anti-Cdc25C 抗体 [E302]

Alexa Fluor® 647 Anti-Cdc25C antibody [E302]

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(1 Publication)

Rabbit Recombinant Monoclonal Cdc25C antibody - conjugated to Alexa Fluor® 647. Suitable for ICC/IF and reacts with Human samples. Cited in 1 publication.

別名を表示する

M-phase inducer phosphatase 3, Dual specificity phosphatase Cdc25C, CDC25C

1 Images
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Cdc25C antibody [E302] (AB205426)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Cdc25C antibody [E302] (AB205426)

ab205426 staining Cdc25C in HeLa cells. The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab205426 at 1/100 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

This product also gave a positive signal under the same testing conditions in HeLa cells fixed with 4% formaldehyde (10 min).

関連する標識済み抗体及び組成の異なる製品 (3)

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

E302

アイソタイプ

IgG

標識

Alexa Fluor® 647

励起波長/蛍光波長

Ex: 650nm, Em: 665nm

キャリアフリー

No

交差種

Human

アプリケーション

ICC/IF

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p>This product gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min) and 100% methanol (5 min)</p>" }, "Rat": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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製品の詳細

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle|Store in the dark
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Cdc25C also known as dual specificity phosphatase Cdc25C is a 65-kDa protein that acts as a cell cycle regulator controlling the transition from the G2 to M phase. The protein is expressed in various tissues but has higher expression levels in actively dividing cells. Cdc25C is important for cell division due to its ability to activate cyclin-dependent kinases (CDKs) by dephosphorylating the inactive phosphorylated forms. This action makes Cdc25C an important target for regulating the cell cycle and ensuring proper cell proliferation.
Biological function summary

The proper function of Cdc25C involves facilitating proper cell cycle progression by coordinating with other cell cycle regulators. It participates in a complex network where it dephosphorylates and activates CDK1/cyclin B1 complexes promoting the mitotic entry. This function is important for maintaining genomic stability during cell division. Misregulation of Cdc25C can lead to cell cycle arrest or uncontrolled cell proliferation highlighting its essential role in cell cycle control mechanisms.

Pathways

Cdc25C fits into the cell cycle checkpoint pathways and is also a part of the DNA damage response pathways. It connects with the p53 and ATM/ATR signaling proteins while responding to DNA damage ensuring a temporary pause in cell cycle progression for repair mechanisms to act. Proper interaction with these pathways is essential for maintaining cellular integrity and preventing the proliferation of damaged cells with CDK1 and Wee1 kinase serving as major interacting proteins in these processes.

Misregulation or overexpression of Cdc25C can relate to cancer and various cell proliferation disorders. In cancer Cdc25C's disordered expression often links to unchecked cell division and tumor progression commonly involving proteins such as p53 which act as tumor suppressors. Furthermore abnormalities in Cdc25C function may lead to issues related to cell cycle progression errors implicating it in other proliferative disorders such as hyperplasia where it acts in conjunction with and alters the activities of proteins like cyclin B1.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Functions as a dosage-dependent inducer in mitotic control. Tyrosine protein phosphatase required for progression of the cell cycle (PubMed : 8119945). When phosphorylated, highly effective in activating G2 cells into prophase (PubMed : 8119945). Directly dephosphorylates CDK1 and activates its kinase activity (PubMed : 8119945).
See full target information CDC25C
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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

PLoS pathogens 21:e1013167 PubMed40359428

2025

Caspase-1-licensed pyroptosis drives dsRNA-mediated necroptosis and dampens host defense against bacterial pneumonia.

Applications

Unspecified application

Species

Unspecified reactive species

Qinyu Luo,Lihua Shen,Shiyue Yang,Yan Zhang,Yihang Pan,Zehua Wu,Qiang Shu,Qixing Chen
View all publications
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代替製品

Primary Antibodies

AB47322

Anti-Cdc25C (phospho S216) antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdc25C (phospho S216) antibody (AB47322)

  • 3
  • 2
Primary Antibodies

AB32050

Anti-Cdc25C antibody [E303]

RabMAb|Recombinant|KO Validated

Western blot - Anti-Cdc25C antibody [E303] (AB32050)

  • 0
  • 0
Primary Antibodies

AB232553

Anti-Cdc25C antibody [E302] - BSA and Azide free

RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdc25C antibody [E302] - BSA and Azide free (AB232553)

  • 0
  • 0

組合せ製品

Primary Antibodies

AB217731

Alexa Fluor® 555 Anti-Cyclin A2 antibody [EPR17351]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 555 Anti-Cyclin A2 antibody [EPR17351] (AB217731)

  • 0
  • 0
Primary Antibodies

AB203448

Alexa Fluor® 555 Anti-Cyclin D1 antibody [EPR2241]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 555 Anti-Cyclin D1 antibody [EPR2241] (AB203448)

  • 0
  • 0
Primary Antibodies

AB109315

Anti-Cdc6 antibody [EPR714(2)]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdc6 antibody [EPR714(2)] (AB109315)

  • 4
  • 2
Primary Antibodies

AB185622

Anti-Cyclin B2/CCNB2 antibody [R17985]

RabMAb|Recombinant|20ul selling size|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-Cyclin B2/CCNB2 antibody [R17985] (AB185622)

  • 0
  • 0

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