AB200317

Alexa Fluor® 647 Anti-CD45 抗体 [EP322Y]

Alexa Fluor® 647 Anti-CD45 antibody [EP322Y]

RabMAb
Recombinant
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(4 Publications)

Anti-CD45 antibody [EP322Y] - Alexa Fluor® 647 conjugated (ab200317) is a rabbit recombinant monoclonal antibody detecting CD45 in Flow Cytometry (Intra), Flow Cytometry. Suitable for Human.

- Biophysical QC for unrivalled batch-batch consistency

別名を表示する

CD45, PTPRC, Receptor-type tyrosine-protein phosphatase C, Leukocyte common antigen, T200, L-CA

1 Images

Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-CD45 antibody [EP322Y] (AB200317)

Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-CD45 antibody [EP322Y] (AB200317)

Overlay histogram showing Jurkat (human T cell leukemia cell line from peripheral blood) cells stained with ab200317 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab200317, 1/50000 dilution) for 30 min at 22°C.

Isotype control antibody (black line) was Rabbit IgG (monoclonal) Alexa Fluor^® 647 (ab199093) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

Acquisition of >5,000 events were collected using a 40 mW Red laser (640nm) and 670/14 bandpass filter.

This antibody gave a positive signal in Jurkat cells fixed with 4% formaldehyde (10 min)/permeabilized with 0.1% PBS-Triton X-100 for 15 min used under the same conditions.

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EP322Y

アイソタイプ

IgG

標識

Alexa Fluor® 647

励起波長/蛍光波長

Ex: 650nm, Em: 665nm

キャリアフリー

交差種

Human

アプリケーション

Flow Cyt (Intra)

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/50000", "FlowCytIntra-species-notes": "<p></p>" } } }

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製品の詳細

What is this antibody validated in?
Alexa Fluor® 647 Anti-CD45 antibody [EP322Y] (ab200317) is a rabbit recombinant monoclonal antibody and is validated for use in Flow Cytometry (Intra), Flow Cytometry (Flow Cyt) in Human samples.

Related products
Antibody clone EP322Y is also available pre-conjugated to a variety of labels for your convenience – Anti-CD45 Alexa Fluor® 647 [EP322Y] (ab200317).

Other related products
We have a range of other formats of antibody clone [EP322Y] also available for your convenience: ab40763, Alexa Fluor® 488 - ab200315, Alexa Fluor® 647 - ab200317, Carrier free - ab214437, PE - ab214501, APC - ab221245, Alexa Fluor® 594 - ab303598

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle|Store in the dark

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

CD45 also known as Protein tyrosine phosphatase receptor type C (PTPRC) is a transmembrane glycoprotein with a molecular mass ranging between 180-240 kDa depending on its isoform. It is expressed on the surface of almost all hematopoietic cells except for mature erythrocytes and platelets. CD45 has a critical role in regulating antigen receptor signaling by modifying kinases involved in signal transduction and it is essential in lymphocyte development and activation. Because of its broad expression on immune cells CD45 is a valuable marker for differentiating various immune cell types in assays like flow cytometry and immunohistochemistry (IHC) often referred to as CD45 stain.

Biological function summary

CD45 acts by regulating tyrosine phosphorylation in the immune cell signaling context participating directly in signal transduction. It functions by dephosphorylating specific phosphotyrosine residues on various proteins modulating the signaling threshold required for lymphocyte activation. Although not known to be part of a protein complex CD45 itself shows isoform variation that associates with specific immune cell types impacting their function. Importantly CD45 interacts with multiple signaling molecules to affect cell growth and differentiation.

Pathways

Scientists associate CD45 with signaling pathways such as the T-cell receptor (TCR) and B-cell receptor (BCR) signaling. CD45 modulates the activity of Src family kinases important elements in these pathways making it essential for effective immune response and tolerance. Furthermore interactions between CD45 and proteins like Lck in T-cells or Lyn in B-cells highlight its pivotal role in executing its signaling functions.

Scientists often link CD45 to immune-related conditions including autoimmunity and leukemia. In autoimmune diseases altered CD45 expression or activity can disrupt normal immune function contributing to pathogenesis. In leukemia CD45 expression levels can assist in disease classification and prognosis as it interacts with proteins involved in cell cycle regulation. The anti-CD45 antibodies can provide diagnostic and therapeutic avenues highlighting their utility in disease management.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor (PubMed : 35767951). Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity (By similarity).. (Microbial infection) Acts as a receptor for human cytomegalovirus protein UL11 and mediates binding of UL11 to T-cells, leading to reduced induction of tyrosine phosphorylation of multiple signaling proteins upon T-cell receptor stimulation and impaired T-cell proliferation.

See full target information PTPRC

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文献 (4)

Recent publications for all applications. Explore the full list and refine your search

Lab on a chip 25:5292-5301 PubMed40878245

2025

Benchmarking microfluidic and immunomagnetic platforms for isolating circulating tumor cells in pancreatic cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Celine Macaraniag,Ifra Khan,Alexandra Barabanova,Valentina Valle,Jian Zhou,Pier C Giulianotti,Alain Borgeat,Gina Votta-Velis,Ian Papautsky

Journal of experimental & clinical cancer research : CR 43:170 PubMed38886756

2024

Intravesical instillation-based mTOR-STAT3 dual targeting for bladder cancer treatment.

Applications

Unspecified application

Species

Unspecified reactive species

Dae Hoon Lee,Jung Ki Yoo,Ki Hwan Um,Wootae Ha,Soo Min Lee,Junseong Park,Min Jeong Kye,Jungyo Suh,Jin Woo Choi

Cell reports. Medicine 3:100525 PubMed35243422

2022

An omic and multidimensional spatial atlas from serial biopsies of an evolving metastatic breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Brett E Johnson,Allison L Creason,Jayne M Stommel,Jamie M Keck,Swapnil Parmar,Courtney B Betts,Aurora Blucher,Christopher Boniface,Elmar Bucher,Erik Burlingame,Todd Camp,Koei Chin,Jennifer Eng,Joseph Estabrook,Heidi S Feiler,Michael B Heskett,Zhi Hu,Annette Kolodzie,Ben L Kong,Marilyne Labrie,Jinho Lee,Patrick Leyshock,Souraya Mitri,Janice Patterson,Jessica L Riesterer,Shamilene Sivagnanam,Julia Somers,Damir Sudar,Guillaume Thibault,Benjamin R Weeder,Christina Zheng,Xiaolin Nan,Reid F Thompson,Laura M Heiser,Paul T Spellman,George Thomas,Emek Demir,Young Hwan Chang,Lisa M Coussens,Alexander R Guimaraes,Christopher Corless,Jeremy Goecks,Raymond Bergan,Zahi Mitri,Gordon B Mills,Joe W Gray

Theranostics 10:11026-11048 PubMed33042268

2020

ALICE: a hybrid AI paradigm with enhanced connectivity and cybersecurity for a serendipitous encounter with circulating hybrid cells.

Applications

Unspecified application

Species

Unspecified reactive species

Kok Suen Cheng,Rongbin Pan,Huaping Pan,Binglin Li,Stephene Shadrack Meena,Huan Xing,Ying Jing Ng,Kaili Qin,Xuan Liao,Benson Kiprono Kosgei,Zhipeng Wang,Ray P S Han

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Alexa Fluor® 647 Anti-CD45 抗体 [EP322Y]

Alexa Fluor® 647 Anti-CD45 antibody [EP322Y]

Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-CD45 antibody [EP322Y] (AB200317)

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Key facts

宿主種

クローン性

クローン番号

アイソタイプ

標識

励起波長/蛍光波長

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (4)

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