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AB303478

Alexa Fluor® 647 Anti-CD16+CD32 抗体 [EPR23501-203]

Alexa Fluor® 647 Anti-CD16+CD32 antibody [EPR23501-203]

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Rabbit Recombinant Monoclonal FCGR3 antibody - conjugated to Alexa Fluor® 647. Suitable for IHC-P, Flow Cyt and reacts with Mouse samples.

別名を表示する

CD16, Low affinity immunoglobulin gamma Fc region receptor III, IgG Fc receptor III, Fc-gamma RIII, FcRIII, Fcgr3

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-CD16+CD32 antibody [EPR23501-203] (AB303478)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-CD16+CD32 antibody [EPR23501-203] (AB303478)

Immunohistochemical analysis of paraffin-embedded mouse lung cancer tissue stained for CD16+CD32 using ab303478 at a 1/100 dilution (5.0 μg/ml) (Red). Positive staining on mouse lung cancer. The section was incubated with ab303478 for 60 mins at room temperature (shown in red). Nuclear DNA was labeled with DAPI (Blue). The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-CD16+CD32 antibody [EPR23501-203] (AB303478)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-CD16+CD32 antibody [EPR23501-203] (AB303478)

Immunohistochemical analysis of paraffin-embedded mouse liver tissue stained for CD16+CD32 using ab303478 at a 1/100 dilution (5.0 μg/ml) (Red). Positive staining on mouse liver. The section was incubated with ab303478 for 60 mins at room temperature (shown in red). Nuclear DNA was labeled with DAPI (Blue). The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins.

Flow Cytometry - Alexa Fluor® 647 Anti-CD16+CD32 antibody [EPR23501-203] (AB303478)
  • Flow Cyt

Lab

Flow Cytometry - Alexa Fluor® 647 Anti-CD16+CD32 antibody [EPR23501-203] (AB303478)

Flow Cytometry analysis of mouse splenocytes using ab303478 at a 1/500 dilution (0.1 μg) (Right panel) compared to Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 647) (ab199093) (Left panel). Gated on viable cells.

Flow Cytometry - Alexa Fluor® 647 Anti-CD16+CD32 antibody [EPR23501-203] (AB303478)
  • Flow Cyt

Lab

Flow Cytometry - Alexa Fluor® 647 Anti-CD16+CD32 antibody [EPR23501-203] (AB303478)

Flow Cytometry analysis of bEnd.3 (Mouse brain endothelioma) (Left panel) and J774A.1 (Mouse reticulum cell sarcoma monocyte macrophage) (Right panel) using ab303478 at a 1/500 dilution (0.1 μg) (Red) compared to Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 647) (ab199093) (Black) and cells without incubation with primary antibody and secondary antibody (Blue). Negative control : bEnd.3 (PMID : 23911392). Gated on viable cells.

関連する標識済み抗体及び組成の異なる製品 (3)

  • Unconjugated

    Anti-CD16+CD32 antibody [EPR23501-203]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-CD16+CD32 antibody [EPR23501-203]

  • Carrier free

    Anti-CD16+CD32 antibody [EPR23501-203] - BSA and Azide free

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR23501-203

アイソタイプ

IgG

標識

Alexa Fluor® 647

励起波長/蛍光波長

Ex: 650nm, Em: 665nm

キャリアフリー

No

交差種

Mouse

アプリケーション

Flow Cyt, IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/500", "FlowCyt-species-notes": "<p></p>" } } }
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製品の詳細

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle|Store in the dark
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

CD16 and CD32 also referred to as FCγRIII and FCγRII are proteins expressed on the surface of various immune cells like natural killer cells macrophages and some subsets of T cells. CD16 has a mass ranging between 50-80 kDa while CD32 exhibits variability in size between 32-40 kDa due to different isoforms. These proteins play pivotal roles in immune response by binding to the Fc region of immunoglobulins influencing cell activation and signaling.
Biological function summary

CD16 and CD32 serve important roles in mediating immune cell interactions. They work as part of immune synapses facilitating antibody-dependent cellular cytotoxicity (ADCC) and phagocytosis processes. Due to their involvement in these complex activities they are included in immunological synapse formation and modulation extending their influence over overall immune regulation and response. These proteins are known to contribute to both innate and adaptive immune mechanisms as receptors for the Fc portion of immunoglobulin G.

Pathways

CD16 and CD32 interact within signaling pathways like the Fc gamma receptor-mediated phagocytosis pathway and the immune response-regulating signaling pathway. These receptors work in coordination with other immunoglobulin family members to initiate targeted cellular reactions necessary for immune challenge responses. CD32 often partners with CD19 and CD21 within pathways to modulate B cell receptor-mediated signaling.

CD16 and CD32 have connections to autoimmune diseases and infectious diseases. Aberrant expression or dysfunction in these proteins can contribute to the development of conditions such as systemic lupus erythematosus where improper immune complex clearance occurs and chronic inflammation results. CD16's involvement with NK cells can also play a role in viral infection control highlighting their importance in pathogen defense mechanisms.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Receptor for the Fc region of complexed immunoglobulins gamma. Low affinity receptor which binds to IgG1, IgG2a and IgG2b (PubMed : 17558411). Mediates neutrophil activation by IgG complexes redundantly with Fcgr4 (PubMed : 18097064).
See full target information Fcgr3

追加のターゲット

Fcgr2,Fcgr2
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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。
保証に関する詳細については利用規約をご確認ください。
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関連製品

Select an associated product type
Alternative Version
Primary Antibodies

AB307622

Alexa Fluor® 488 Anti-CD16+CD32 antibody [EPR23501-203]

primary-antibodies

alexa-fluor-488-cd16cd32-antibody-epr23501-203-ab307622

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(0 reviews)

Alternative Version
Primary Antibodies

AB275095

Anti-CD16+CD32 antibody [EPR23501-203] - BSA and Azide free

primary-antibodies

cd16cd32-antibody-epr23501-203-bsa-and-azide-free-ab275095

0

(0 reviews)

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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