Alexa Fluor® 647 Anti-CD147 抗体 [EPR18008-8] (ab302695)
![Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-CD147 antibody [EPR18008-8] (AB302695)](https://www.abcam.co.jp/ps/products/302/ab302695/Images/AB302695-490075-anti-cd147-antibody-epr180088-icc-wehi-231.jpg "Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-CD147 antibody [EPR18008-8] (AB302695)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Alexa Fluor® 647 Rabbit monoclonal [EPR18008-8] to CD147
- Suitable for: ICC/IF, Flow Cyt
- Reacts with: Mouse
- Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm
Related conjugates and formulations
製品の概要
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製品名
Alexa Fluor® 647 Anti-CD147 antibody [EPR18008-8]
CD147 一次抗体 製品一覧 -
製品の詳細
Alexa Fluor® 647 Rabbit monoclonal [EPR18008-8] to CD147 -
由来種
Rabbit -
標識
Alexa Fluor® 647. Ex: 652nm, Em: 668nm -
アプリケーション
適用あり: ICC/IF, Flow Cytmore details -
種交差性
交差種: Mouse -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- ICC/IF: WEHI-231 (mouse B cell lymphoma B lymphocytes). Flow Cyt: Mouse thymocytes.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR18008-8 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab302695の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF |
1/50.
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| Flow Cyt |
1/50.
|
| 特記事項 |
|---|
|
ICC/IF
1/50. |
|
Flow Cyt
1/50. |
ターゲット情報
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機能
Plays pivotal roles in spermatogenesis, embryo implantation, neural network formation and tumor progression. Stimulates adjacent fibroblasts to produce matrix metalloproteinases (MMPS). May target monocarboxylate transporters SLC16A1, SLC16A3 and SLC16A8 to plasma membranes of retinal pigment epithelium and neural retina. Seems to be a receptor for oligomannosidic glycans. In vitro, promotes outgrowth of astrocytic processes. -
組織特異性
Present only in vascular endothelium in non-neoplastic regions of the brain, whereas it is present in tumor cells but not in proliferating blood vessels in malignant gliomas. -
配列類似性
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
翻訳後修飾
N-glycosylated. -
細胞内局在
Cell membrane. Melanosome. Colocalizes with SLC16A1 and SLC16A8 (By similarity). Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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参照データベース
- Entrez Gene: 12215 Mouse
- SwissProt: P18572 Mouse
- Unigene: 431532 Mouse
- Unigene: 726 Mouse
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別名
- 5A11 antigen antibody
- 5F7 antibody
- BASI_HUMAN antibody
see all
画像
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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-CD147 antibody [EPR18008-8] (AB302695)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized WEHI-231 (mouse B cell lymphoma B lymphocyte) cells labeling CD147 with ab302695 at 1/50 dilution (Red). Confocal image showing membranous staining in WEHI-231 cells. The nuclear counter stain is DAPI (blue).
Tubulin is detected with ab195887 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 488) at 1:200 dilution (2.5 μg/mL) (Green).
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![Flow Cytometry - Alexa Fluor® 647 Anti-CD147 antibody [EPR18008-8] (AB302695)](https://www.abcam.co.jp/ps/products/302/ab302695/Images/AB302695-490074-anti-cd147-antibody-epr180088-flow-cytometry-mouse-thymocytes.jpg)
Flow Cytometry - Alexa Fluor® 647 Anti-CD147 antibody [EPR18008-8] (AB302695)Flow cytometric overlay histogram showing Mouse thymocytes labeling CD147 with ab302695 at 1/50 dilution (red). Isotype control antibody (black) was Rabbit monoclonal isotype control - Alexa Fluor® 647 used at the same concentration and conditions as the primary antibody. Unlabeled sample (blue) was also used as a control.
Gated on viable cells.
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![Alexa Fluor® 647 Anti-CD147 antibody [EPR18008-8] (AB302695)](https://www.abcam.co.jp/ps/products/302/ab302695/Images/AB302695-1-Benefits-of-Recombinant-antibodies.jpg)
Alexa Fluor® 647 Anti-CD147 antibody [EPR18008-8] (AB302695)
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab302695 は論文での使用が確認できていません。