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リコンビナントRabMAb

Alexa Fluor® 647 Anti-CD14 抗体 [SP192] (ab302698)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-CD14 antibody [SP192] (AB302698)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-CD14 antibody [SP192] (AB302698)
  • Flow Cytometry - Alexa Fluor® 647 Anti-CD14 antibody [SP192] (AB302698)
  • Alexa Fluor® 647 Anti-CD14 antibody [SP192] (AB302698)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Alexa Fluor® 647 Rabbit monoclonal [SP192] to CD14
  • Suitable for: Flow Cyt, IHC-P
  • Reacts with: Human
  • Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 594 APC Carrier Free PE Unconjugated

製品の概要

  • 製品名

    Alexa Fluor® 647 Anti-CD14 antibody [SP192]
    CD14 一次抗体 製品一覧

  • 製品の詳細

    Alexa Fluor® 647 Rabbit monoclonal [SP192] to CD14

  • 由来種

    Rabbit

  • 標識

    Alexa Fluor® 647. Ex: 652nm, Em: 668nm

  • アプリケーション

    適用あり: Flow Cyt, IHC-Pmore details

  • 種交差性

    交差種: Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • IHC-P: Human tonsil and lung carcinoma FFPE tissue sections. Flow Cyt: Human peripheral blood mononuclear cell (PBMC).

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab302698の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt
1/500.
IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
特記事項
Flow Cyt
1/500.
IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

ターゲット情報

  • 機能

    Cooperates with MD-2 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules.

  • 組織特異性

    Expressed strongly on the surface of monocytes and weakly on the surface of granulocytes; also expressed by most tissue macrophages.

  • 配列類似性

    Contains 11 LRR (leucine-rich) repeats.

  • 翻訳後修飾

    N- and O- glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan.

  • 細胞内局在

    Cell membrane.
  • Information by UniProt

  • 参照データベース

    • 別名

      • CD 14 antibody
      • CD_antigen=CD14 antibody
      • CD14 antibody
      • CD14 antigen antibody
      • CD14 molecule antibody
      • CD14_HUMAN antibody
      • LPS-R antibody
      • Mo2 antibody
      • Monocyte differentiation antigen CD14 antibody
      • Monocyte differentiation antigen CD14 urinary form antibody
      • Monocyte differentiation antigen CD14, membrane-bound form antibody
      • Myeloid cell specific leucine rich glycoprotein antibody
      • Myeloid cell-specific leucine-rich glycoprotein antibody
      see all

    画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-CD14 antibody [SP192] (AB302698)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-CD14 antibody [SP192] (AB302698)

      Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue labeling CD14 with ab302698 at 1/100 dilution (5.0 µg/mL). Membraneous staining on human lung carcinoma is observed. The section was incubated with ab302698 for 60 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. The nuclear counterstain was DAPI (Blue). Negative control: Primary diluent was used instead of primary antibody. The section was then mounted using Fluoromount®

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 40 mins.

      Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-CD14 antibody [SP192] (AB302698)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-CD14 antibody [SP192] (AB302698)

      Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling CD14 with ab302698 at 1/100 dilution (5.0 µg/mL). Membraneous staining on human tonsil is observed. The section was incubated with ab302698 for 60 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. The nuclear counterstain was DAPI (Blue). Negative control: Primary diluent was used instead of primary antibody. The section was then mounted using Fluoromount®

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 40 mins.

      Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    • Flow Cytometry - Alexa Fluor® 647 Anti-CD14 antibody [SP192] (AB302698)
      Flow Cytometry - Alexa Fluor® 647 Anti-CD14 antibody [SP192] (AB302698)

      Flow cytometric analysis of Human peripheral blood mononuclear cell (PBMC) (Right) and Rabbit monoclonal isotype control - Alexa Fluor® 647 (Left) used at the same concentration and conditions as the primary antibody. Ab302698 used at 1/500 dilution (0.1μg).

      Gated on viable cells.

    • Alexa Fluor® 647 Anti-CD14 antibody [SP192] (AB302698)
      Alexa Fluor® 647 Anti-CD14 antibody [SP192] (AB302698)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab302698 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab302698 は論文での使用が確認できていません。

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