Alexa Fluor® 647 Anti-CD130 (gp130) 抗体 [EPR24557-5] (ab300159)
![Flow Cytometry - Alexa Fluor® 647 Anti-CD130 (gp130) antibody [EPR24557-5] (AB300159)](https://www.abcam.co.jp/ps/products/300/ab300159/Images/AB300159-486131-anti-CD130-EPR245575-gp130-flow-cytometry-A549.jpg "Flow Cytometry - Alexa Fluor® 647 Anti-CD130 (gp130) antibody [EPR24557-5] (AB300159)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Alexa Fluor® 647 Rabbit monoclonal [EPR24557-5] to CD130 (gp130)
- Suitable for: Flow Cyt
- Knockout validated
- Reacts with: Human
- Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm
Related conjugates and formulations
製品の概要
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製品名
Alexa Fluor® 647 Anti-CD130 (gp130) antibody [EPR24557-5]
CD130 (gp130) 一次抗体 製品一覧 -
製品の詳細
Alexa Fluor® 647 Rabbit monoclonal [EPR24557-5] to CD130 (gp130) -
由来種
Rabbit -
標識
Alexa Fluor® 647. Ex: 652nm, Em: 668nm -
アプリケーション
適用あり: Flow Cytmore details -
種交差性
交差種: Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- Flow Cyt: Wild-type A549 cells.
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特記事項
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, 68% PBS -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR24557-5 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab300159の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Flow Cyt |
1/50.
|
| 特記事項 |
|---|
|
Flow Cyt
1/50. |
ターゲット情報
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機能
Signal-transducing molecule. The receptor systems for IL6, LIF, OSM, CNTF, IL11, CTF1 and BSF3 can utilize gp130 for initiating signal transmission. Binds to IL6/IL6R (alpha chain) complex, resulting in the formation of high-affinity IL6 binding sites, and transduces the signal. Does not bind IL6. May have a role in embryonic development (By similarity). The type I OSM receptor is capable of transducing OSM-specific signaling events. -
組織特異性
Found in all the tissues and cell lines examined. Expression not restricted to IL6 responsive cells. -
配列類似性
Belongs to the type I cytokine receptor family. Type 2 subfamily.
Contains 5 fibronectin type-III domains.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain. -
ドメイン
The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
The box 1 motif is required for JAK interaction and/or activation. -
翻訳後修飾
Phosphorylation of Ser-782 down-regulates cell surface expression.
Heavily N-glycosylated. -
細胞内局在
Secreted and Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 3572 Human
- Omim: 600694 Human
- SwissProt: P40189 Human
- Unigene: 532082 Human
- Unigene: 706627 Human
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別名
- CD130 antibody
- CD130 antigen antibody
- CDw130 antibody
see all
画像
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Flow Cytometry - Alexa Fluor® 647 Anti-CD130 (gp130) antibody [EPR24557-5] (AB300159)
Flow Cytometry analysis of Wild-type A549 (human lung carcinoma epithelial cells, Right) / CD130 (gp130) knockout A549 cells (Left) surface-labelling CD130 (gp130) (red) with ab300159 at a 1/50 dilution. Rabbit monoclonal isotype control - Alexa Fluor® 647 (Black). Unlabelled control - Cells without incubation with primary or secondary antibodies (Blue).
Gated on viable cells.
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![Alexa Fluor® 647 Anti-CD130 (gp130) antibody [EPR24557-5] (AB300159)](https://www.abcam.co.jp/ps/products/300/ab300159/Images/AB300159-2-Benefits-of-Recombinant-antibodies.jpg)
Alexa Fluor® 647 Anti-CD130 (gp130) antibody [EPR24557-5] (AB300159)
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab300159 は論文での使用が確認できていません。