Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
リコンビナントRabMAb

Alexa Fluor® 647 Anti-Bassoon/BSN 抗体 [EPR25379-3] (ab300673)

Submit a review Submit a question
Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-Bassoon/BSN antibody [EPR25379-3] (AB300673)
  • Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-Bassoon/BSN antibody [EPR25379-3] (AB300673)
  • Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-Bassoon/BSN antibody [EPR25379-3] (AB300673)
  • Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-Bassoon/BSN antibody [EPR25379-3] (AB300673)
  • Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-Bassoon/BSN antibody [EPR25379-3] (AB300673)
  • Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-Bassoon/BSN antibody [EPR25379-3] (AB300673)
  • Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-Bassoon/BSN antibody [EPR25379-3] (AB300673)
  • Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-Bassoon/BSN antibody [EPR25379-3] (AB300673)
  • Alexa Fluor® 647 Anti-Bassoon/BSN antibody [EPR25379-3] (AB300673)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Alexa Fluor® 647 Rabbit monoclonal [EPR25379-3] to Bassoon/BSN
  • Suitable for: IHC-Fr
  • Reacts with: Mouse, Rat
  • Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm

Conjugates logo Related conjugates and formulations

Carrier Free Unconjugated

製品の概要

  • 製品名

    Alexa Fluor® 647 Anti-Bassoon/BSN antibody [EPR25379-3]
    Bassoon/BSN 一次抗体 製品一覧

  • 製品の詳細

    Alexa Fluor® 647 Rabbit monoclonal [EPR25379-3] to Bassoon/BSN

  • 由来種

    Rabbit

  • 標識

    Alexa Fluor® 647. Ex: 652nm, Em: 668nm

  • アプリケーション

    適用あり: IHC-Frmore details
    適用なし: IHC-P

  • 種交差性

    交差種: Mouse, Rat

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • IHC-Fr: Mouse and rat cerebellum (fresh).

  • 特記事項

    ab300673 does not react in IHC-P with mouse and rat species. 

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark.

  • バッファー

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPR25379-3

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab300673の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-Fr
1/100.
特記事項
IHC-Fr
1/100.
追加情報
Is unsuitable for IHC-P.

ターゲット情報

  • 機能

    Is thought to be involved in the organization of the cytomatrix at the nerve terminals active zone (CAZ) which regulates neurotransmitter release. Seems to act through binding to ERC2/CAST1. Essential in regulated neurotransmitter release from a subset of brain glutamatergic synapses. Involved in the formation of the retinal photoreceptor ribbon synapses.

  • 組織特異性

    Exclusively expressed in brain.

  • 翻訳後修飾

    Myristoylated. The N-terminal myristoylation is not sufficient for presynaptic localization.

  • 細胞内局在

    Cytoplasm. Cell junction > synapse > synaptosome. Cytoplasm > cytoskeleton. Localized to the active zone of presynaptic density.
  • Information by UniProt

  • 参照データベース

    • 別名

      • Bassoon protein antibody
      • bsn antibody
      • BSN_HUMAN antibody
      • KIAA0434 antibody
      • Neuronal double zinc finger protein antibody
      • Presynaptic cytomatrix protein antibody
      • Protein bassoon antibody
      • Zinc finger protein 231 antibody
      • ZNF 231 antibody
      • ZNF231 antibody
      see all

    画像

    • Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-Bassoon/BSN antibody [EPR25379-3] (AB300673)
      Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-Bassoon/BSN antibody [EPR25379-3] (AB300673)

      Immunohistochemical analysis of 4% PFA-fixed/ 0.2% Triton X-100 permeabilized frozen mouse cerebellum (fresh) tissue labeling Bassoon/BSN with ab300673 at 1/100 dilution (10.0 µg/ml). Positive staining (Red) on mouse cerebellum is observed. The nuclear counterstain was DAPI (Blue).

    • Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-Bassoon/BSN antibody [EPR25379-3] (AB300673)
      Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-Bassoon/BSN antibody [EPR25379-3] (AB300673)

      Immunohistochemical analysis of 4% PFA-fixed/ 0.2% Triton X-100 permeabilized frozen rat cerebellum (fresh) tissue labeling Bassoon/BSN with ab300673 at 1/100 dilution (10.0 µg/ml). Positive staining (Red) on rat cerebellum is observed. The nuclear counterstain was DAPI (Blue).

    • Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-Bassoon/BSN antibody [EPR25379-3] (AB300673)
      Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-Bassoon/BSN antibody [EPR25379-3] (AB300673)

      Immunohistochemical analysis of 4% PFA-fixed/ 0.2% Triton X-100 permeabilized frozen mouse liver (fresh) tissue labeling Bassoon/BSN with ab300673 at 1/100 dilution (10.0 µg/ml). Negative control: No staining on mouse liver (PMID: 9679147). The nuclear counterstain was DAPI (Blue).

    • Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-Bassoon/BSN antibody [EPR25379-3] (AB300673)
      Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-Bassoon/BSN antibody [EPR25379-3] (AB300673)

      Immunohistochemical analysis of 4% PFA-fixed/ 0.2% Triton X-100 permeabilized frozen rat liver (fresh) tissue labeling Bassoon/BSN with ab300673 at 1/100 dilution (10.0 µg/ml). Negative control: No staining on rat liver (PMID: 9679147) . The nuclear counterstain was DAPI (Blue).

    • Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-Bassoon/BSN antibody [EPR25379-3] (AB300673)
      Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-Bassoon/BSN antibody [EPR25379-3] (AB300673)

      Immunohistochemical analysis of 4% PFA-fixed/ 0.2% Triton X-100 permeabilized frozen mouse heart (fresh) tissue labeling Bassoon/BSN with ab300673 at 1/100 dilution (5.0 µg/ml). Negative control: No staining on mouse cardiac muscle (PMID: 9679147). The nuclear counterstain was DAPI (Blue).

    • Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-Bassoon/BSN antibody [EPR25379-3] (AB300673)
      Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-Bassoon/BSN antibody [EPR25379-3] (AB300673)

      Immunohistochemical analysis of 4% PFA-fixed/ 0.2% Triton X-100 permeabilized frozen rat heart (fresh) tissue labeling Bassoon/BSN with ab300673 at 1/100 dilution (5.0 µg/ml). Negative control: No staining on rat cardiac muscle (PMID: 9679147). The nuclear counterstain was DAPI (Blue).

    • Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-Bassoon/BSN antibody [EPR25379-3] (AB300673)
      Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-Bassoon/BSN antibody [EPR25379-3] (AB300673)

      Immunohistochemical analysis of 4% PFA-fixed/ 0.2% Triton X-100 permeabilized frozen mouse lung (fresh) tissue labeling Bassoon/BSN with ab300673 at 1/100 dilution (5.0 µg/ml). Negative control: No staining on mouse lung (PMID: 25409824). The nuclear counterstain was DAPI (Blue).

    • Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-Bassoon/BSN antibody [EPR25379-3] (AB300673)
      Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-Bassoon/BSN antibody [EPR25379-3] (AB300673)

      Immunohistochemical analysis of 4% PFA-fixed/ 0.2% Triton X-100 permeabilized frozen rat lung (fresh) tissue labeling Bassoon/BSN with ab300673 at 1/100 dilution (5.0 µg/ml). Negative control: No staining on rat lung (PMID: 25409824). The nuclear counterstain was DAPI (Blue).

    • Alexa Fluor® 647 Anti-Bassoon/BSN antibody [EPR25379-3] (AB300673)
      Alexa Fluor® 647 Anti-Bassoon/BSN antibody [EPR25379-3] (AB300673)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab300673 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab300673 は論文での使用が確認できていません。

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    There are currently no Customer reviews or Questions for ab300673.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com