Alexa Fluor® 647 Anti-Aquaporin 4 抗体 [EPR24281-65] (ab290986)

Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling Aquaporin 4 with ab290986.

Positive staining on human cerebrum. The section was then incubated overnight at +4°C in TBS containing 0.025% (v/v) Triton X-100 and 1% (w/v) BSA with ab290986 at 1/100 dilution (shown in red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount^®. Non-specific protein-protein interactions were then blocked in TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 1% (w/v) BSA for 1h at room temperature. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling Aquaporin 4 with ab290986.

Positive staining on mouse cerebrum. The section was then incubated overnight at +4°C in TBS containing 0.025% (v/v) Triton X-100 and 1% (w/v) BSA with ab290986 at 1/100 dilution (shown in red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount^®. Non-specific protein-protein interactions were then blocked in TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 1% (w/v) BSA for 1h at room temperature. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

Immunohistochemical analysis of paraffin-embedded Mouse retina tissue labeling Aquaporin 4 with ab290986.

Positive staining on mouse retina. The section was then incubated overnight at +4°C in TBS containing 0.025% (v/v) Triton X-100 and 1% (w/v) BSA with ab290986 at 1/100 dilution (shown in red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount^®. Non-specific protein-protein interactions were then blocked in TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 1% (w/v) BSA for 1h at room temperature. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling Aquaporin 4 with ab290986.

Positive staining on rat cerebrum. The section was then incubated overnight at +4°C in TBS containing 0.025% (v/v) Triton X-100 and 1% (w/v) BSA with ab290986 at 1/100 dilution (shown in red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount^®. Non-specific protein-protein interactions were then blocked in TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 1% (w/v) BSA for 1h at room temperature. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labeling Aquaporin 4 with ab290986.

Negative control: no staining on mouse testis. The section was then incubated overnight at +4°C in TBS containing 0.025% (v/v) Triton X-100 and 1% (w/v) BSA with ab290986 at 1/100 dilution (shown in red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount^®. Non-specific protein-protein interactions were then blocked in TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 1% (w/v) BSA for 1h at room temperature. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

Immunohistochemical analysis of 4% PFA-fixed 0.2% Triton X-100 permeabilized frozen sectioned Mouse cerebrum (fresh) tissue labeling Aquaporin 4 with ab290986 at 1/100 dilution. Positive staining on mouse cerebrum (Red). The nuclear counterstain is DAPI (blue).

Immunohistochemical analysis of 4% PFA-fixed 0.2% Triton X-100 permeabilized frozen sectioned Rat cerebrum (fresh) tissue labeling Aquaporin 4 with ab290986 at 1/500 dilution. Positive staining on rat cerebrum (Red). The nuclear counterstain is DAPI (blue).

Immunohistochemical analysis of 4% PFA-fixed 0.2% Triton X-100 permeabilized frozen sectioned Mouse testis (fresh) tissue with ab290986 at 1/100 dilution. Negative control (PMID: 16840793). No staining on mouse testis. Nuclear counterstain is DAPI (blue).